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Trieasy

Manufactured by Yeasen
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Sourced in China

TRIeasy is a versatile lab equipment designed for efficient RNA extraction. It utilizes a combination of chemical and mechanical methods to isolate high-quality RNA from a variety of biological samples.

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Lab products found in correlation

Abi quantstudio 6 flex system, by Thermo Fisher Scientific (1 mentions) Hifair first strand cdna synthesis kit, by Yeasen (1 mentions) Perfectstart green qpcr supermix, by Transgene (1 mentions) M mlv reverse transcriptase, by Promega (1 mentions) Lightcycler 96, by Roche (1 mentions) Hieff qpcr sybr green master mix, by Yeasen (1 mentions)

2 protocols using trieasy

1

Transcriptomic Analysis of Nanoparticle Exposure

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Total RNAs were extracted from control and SPc nanoparticles exposed third instar larvae using TRIeasy (Yeasen Biotech, Shanghai, China). The cDNAs were synthesized by the Hifair First Strand cDNA Synthesis Kit (Yeasen Biotech) and used as templates for PCR experiments using the Perfect Start Green qPCR Super Mix (TransGen Biotech, Beijing, China). qRT-PCR were conducted on an ABI QuantStudio 6 Flex System (Thermo Fisher, USA). The Rpl32 gene was used as internal control for qRT-PCR, and the gene expression level was examined by the ΔΔCt method [88 (link), 92 (link)]. The primers used for qRT-PCR in this study are listed in Additional file 6: Table S2.
Yan S., Li N., Guo Y., Chen Y., Ji C., Yin M., Shen J, & Zhang J. (2022). Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster. Journal of Nanobiotechnology, 20, 515.
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2

Quantitative Transcriptome Analysis Protocol

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Total RNA was extracted from cells using TRIeasy (Yeasen Biotech). Reverse transcription was performed by M-MLV Reverse Transcriptase (Promega). qPCR was performed by a LightCycler 96 (Roche, Basel, Switzerland) with a Hieff qPCR SYBR Green Master Mix (Yeasen Biotech). All primers are listed in Table S2.
Zou G., Zhang X., Wang L., Li X., Xie T., Zhao J., Yan J., Wang L., Ye H., Jiao S., Xiang R, & Shi Y. (2020). Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. Theranostics, 10(15), 6839-6853.
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