Anti p plcγ1

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-p-PLCγ1 is a lab equipment product that detects and binds to the phosphorylated form of phospholipase C gamma 1 (PLCγ1), a signaling enzyme involved in various cellular processes. This product can be used to analyze the activation state of PLCγ1 in cell samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti p p38, by Cell Signaling Technology (2 mentions) Anti p jnk, by Cell Signaling Technology (2 mentions) Anti plcγ1, by Cell Signaling Technology (2 mentions) Cd4 pe, by BD (1 mentions) Cd8 fitc, by BD (1 mentions) Cd25 fitc, by BD (1 mentions) Cd8 apc, by Thermo Fisher Scientific (1 mentions) Cd3 bv421, by BioLegend (1 mentions) Anti mouse igg1 pe, by BD (1 mentions) Anti cd16 32, by Thermo Fisher Scientific (1 mentions) Anti slp76, by BD (1 mentions) Anti pan 14 3 3, by Santa Cruz Biotechnology (1 mentions) Anti 14 3 3ζ, by Santa Cruz Biotechnology (1 mentions) Anti perk1 2, by Merck Group (1 mentions) Cd4 pe vio770, by Miltenyi Biotec (1 mentions) Supersignal west dura, by Thermo Fisher Scientific (1 mentions) Anti α tubulin, by Santa Cruz Biotechnology (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

Spelling variants of this product

PLCγ1 (37 citations) Anti-PLCγ1 (19 citations) P-PLCγ1 (11 citations)

7 protocols using anti p plcγ1

Comprehensive T-cell Signaling Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies and reagents used for flow cytometry were: CD8-APC, Fixable viability stain 450, antiCD16/32 (eBiosciences); anti-rabbit-PE (Jackson Immunoresearch); anti-SLP76 (pY128), CD4-PE, CD25-FITC, CD8 FITC and anti-mouse IgG1-PE (BD Biosciences); CD4-PEVio770 (Miltenyi Biotec); CD3-BV421 (Biolegend); anti-pErk1/2 (pT202/pY204) (Cell signaling Technology). The following reagents were used for cell stimulation, immunoblotting or immunoprecipitation: anti-CD3-biontinylated, anti-CD28-biontinylated, anti-CD3ε, anti-CD28 (eBiosciences); streptavidin (Sigma); anti-pErk1/2 (pT202/pY204), anti-pPLCγ1 (pY783), anti-pp38 (pT180/pY182), anti-pJNK (pT183/Y185), anti-pAkt (pS473), anti-pSLP76 (pS376) (Cell Signaling Technology); anti-GADS (Santa Cruz Biotechnology Inc.); rabbit anti-SLP76 or goat-anti-SLP76 (Thermo Fisher Scientific); anti-β-tubulin (Chemicon); for anti-14-3-3 immunoblotting we used a mix of anti-pan 14-3-3 and anti-14-3-3ζ (Santa Cruz, Biotechnology Inc.).

Navas V.H., Cuche C., Alcover A, & Di Bartolo V. (2017). Serine Phosphorylation of SLP76 Is Dispensable for T Cell Development but Modulates Helper T Cell Function. PLoS ONE, 12(1), e0170396.

+ Open protocol

+ Expand

Immunoblot Analysis of Cell Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorted cells or stimulated cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 x Roche Complete Protease Inhibitor Cocktail, and 1 x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce). Equal amounts of protein samples were separated by 9% SDS-PAGE and transferred onto nitrocellulose membranes (Bio-Rad). The following primary antibodies were used: anti-CIC (homemade) (Kim et al., 2015 (link)), anti-PLCγ1 (#5690, Cell Signaling), anti-p-PLCγ1 (#2821, Cell Signaling), anti-ZAP-70 (#2705, Cell Signaling), anti-p-ZAP-70 (#2701, Cell Signaling Technology), anti-JNK (#9252, Cell Signaling), anti-p-JNK (#9251, Cell Signaling), anti-ERK (#9102, Cell Signaling), anti-p-ERK (#4370, Cell Signaling), anti-p38 (#9212, Cell Signaling), anti-p-p38 (#9211, Cell Signaling), anti-β-actin (#sc-47778, Santa Cruz), and anti-α-tubulin (#sc-398103, Santa Cruz). Membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (HRP) and developed using Clarity Western ECL Substrate (Bio-Rad) or SuperSignal West Dura (Thermo Scientific). Images were acquired using an ImageQuant LAS 500 instrument (GE Healthcare).

Kim S., Park G.Y., Park J.S., Park J., Hong H, & Lee Y. (2021). Regulation of positive and negative selection and TCR signaling during thymic T cell development by capicua. eLife, 10, e71769.

+ Open protocol

+ Expand

Detecting Phosphorylated PLCγ1 in Transfected NIH 3T3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The transfected NIH 3T3 cells were lysed in whole cell extract buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 0.2 mM EDTA, 10 mM Na₃VO₄, 10% glycerol, protease inhibitors). Proteins were separated by gel electrophoresis using 4–12% NuPAGE Bis-Tris gels (Genscript) followed by transfer to nitrocellulose membrane. Membranes were incubated with 5% milk in TBST (0.5 M NaCl, Tris-HCl, pH 7.5, 0.1% (v/v) Tween-20) for 60 min and washed once with TBST. Proteins of interest were detected by incubating membranes over night at 4°C in blocking buffer with anti-p-PLCγ1 (Cell Signaling), anti-PLCγ1 (Cell Signaling), or anti-β-Actin (Sigma), washing with TBST three times for 10 min and incubating with horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Cell Signaling). Blotting signaling was detected with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher).

Chen Z., Luo J., Li J., Kim G., Stewart A., Urban JF J.r., Huang Y., Chen S., Wu L.G., Chesler A., Trinchieri G., Li W, & Wu C. (2020). Interleukin-33 promotes serotonin release from enterochromaffin cells for intestinal homeostasis. Immunity, 54(1), 151-163.e6.

+ Open protocol

+ Expand

Withaferin A Attenuates Pulmonary Fibrosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Withaferin A was procured from Aptus laboratories (Hyderabad, India) and TGF-β1 was obtained from Bio-legend (United States); Bleomycin sulfate was procured from Cipla labs (India); Masson’s trichrome staining kit, Sirius red, Chloramine-T, Hydroxyproline, and Ehrlich reagent were procured from Sigma-Aldrich, Anti-ZO-1, anti-E-cadherin, anti-Smad 2/3, anti-p Smad 2/3, anti-vimentin, anti-NF-κB p65, anti-p VEGF, anti-p p38 MAPK, anti-p FAK, and anti-p PLCγ1 were procured from Cell Signaling Technology, while anti-Col 1A2, anti-Col 3A1, anti-smooth muscle actin, anti-CTGF, anti-fibronectin, and anti-TGF-β1 were obtained from Santa Cruz Biotechnology (United States). ELISA kits were purchased from eBioscience, United States. TGF-β bioplex kit was procured from Merck-Millipore. Rest of the chemicals and reagents were of analytical grade and obtained from commercially available sources.

Bale S., Venkatesh P., Sunkoju M, & Godugu C. (2018). An Adaptogen: Withaferin A Ameliorates in Vitro and in Vivo Pulmonary Fibrosis by Modulating the Interplay of Fibrotic, Matricelluar Proteins, and Cytokines. Frontiers in Pharmacology, 9, 248.

+ Open protocol

+ Expand

FGFR Kinase Phosphorylation of PLC-γ1

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

In total, 2 μM of PLC-γ1 was incubated with 2 μM of a FGFR kinase domain in phosphorylation buffer (20 mM HEPES (pH 7.4), 100 mM NaCl, 20 mM ATP, 20 mM MgCl₂). The FGFR kinase domain was provided from Dr Mohammadi’s lab at NYU and was purified by three-step purification through Ni-affinity column, ion-exchange column, and size exclusion column (10 (link)). In the time-course experiment to verify the maximum phosphorylation, the degree of phosphorylation of PLC-γ1 at Tyr783 was measured by Western blot using anti-pPLC-γ1 (#2821, Cell Signaling) and detected with anti-rabbit IgG-HRP (NXA934, Amersham GE)]. The chemiluminescent signal was detected by Chemidoc (Bio-Rad) and analyzed by Image Lab (Bio-Rad).

Wada J., Rathnayake U., Jenkins L.M., Singh A., Mohammadi M., Appella E., Randazzo P.A, & Samelson L.E. (2022). In vitro reconstitution reveals cooperative mechanisms of adapter protein-mediated activation of phospholipase C-γ1 in T cells. The Journal of Biological Chemistry, 298(3), 101680.

+ Open protocol

+ Expand

Erdr1 Protein Purification and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

PerCP-Cy5.5-conjugated anti-CD4 (RM4-5), anti-CD3ε (145-2C11), and anti-hamster IgG1 (G94-56) were purchased from BD Biosciences (San Diego, CA, USA). APC-conjugated anti-CD69 (H1.2F3) was obtained from eBioscience (San Diego, CA, USA). Alexa488-conjugated anti-CD4 (GK1.5) was obtained from BioLend (San Diego, CA, USA). Anti-pPLCγ1 and anti-NFAT1 were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-PLCγ1 was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Recombinant Erdr1 was prepared as described previously [38 (link)]. In brief, the bacterial vector with the Erdr1 CDS region was constructed from the Erdr1-pCMV-SPORT6 plasmid (Open Biosystems, Huntsville, AL, USA), and then Erdr1 was purified with over 95% purity from bacteria. Lots with low endotoxin levels (<0.1 EU/mL) determined by the Limulus Amebocyte lysate assay (Cape Cod, East Falmouth, MA, USA) were used for experiments.

Kim M.S., Park D., Lee S., Park S., Kim K.E., Kim T.S., Park H.J, & Cho D. (2022). Erythroid Differentiation Regulator 1 Strengthens TCR Signaling by Enhancing PLCγ1 Signal Transduction Pathway. International Journal of Molecular Sciences, 23(2), 844.

+ Open protocol

+ Expand

Comprehensive Anticancer Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

FAP antibody was purchased from Cell Signaling (catalog 66562). DOXO (catalog D1515), PTX (catalog T7402), and CCR2 antagonist (catalog 227016) were purchased from Sigma-Aldrich. Navitoclax (ABT-263) (catalog S1001) was purchased from Selleckchem. Recombinant IL-6 protein (catalog 7270-IL), recombinant WNT5A protein were acquired from R&D System (catalog 645-WN). Tocilizumab was acquired from Novus Biologicals (catalog NBP2-75193). Puromycin was acquired from Thermo Fisher Scientific (catalog A11138). Cell Signaling Technology antibodies contributed: anti–PKC-α (catalog 59754S), anti–p-PKC-α/β II (catalog 9375S), anti–p-PLC-γ1 (catalog 8713S), anti–PLC-γ1 XP (catalog 5690S), anti–p-RAC1/cdc42 (Ser71) (catalog 2461), anti-RAC1/2/3 (catalog 2465), anti-WNT5A/B (catalog MA5-15502), and anti–cleaved caspase-3 (catalog 9661S). Mouse monoclonal β-actin–HRP (catalog 47778, Santa Cruz Biotechnology) was used as loading control. Anti-Ki67 was acquired by BD Horizon (catalog 565929).

Augimeri G., Gonzalez M.E., Paolì A., Eido A., Choi Y., Burman B., Djomehri S., Karthikeyan S.K., Varambally S., Buschhaus J.M., Chen Y.C., Mauro L., Bonofiglio D., Nesvizhskii A.I., Luker G.D., Andò S., Yoon E, & Kleer C.G. (2023). A hybrid breast cancer/mesenchymal stem cell population enhances chemoresistance and metastasis. JCI Insight, 8(18), e164216.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!