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Il 17 and gm csf elispot antibody pairs

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The IL-17 and GM-CSF ELISPOT antibody pairs are designed for the detection and quantification of cells secreting interleukin-17 (IL-17) and granulocyte-macrophage colony-stimulating factor (GM-CSF) using the Enzyme-Linked Immunospot (ELISPOT) assay. These antibody pairs provide the necessary components to perform this specific immunoassay.

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Lab products found in correlation

Nbt bcip substrate, by Roche (2 mentions) Streptavidin hrp, by BioLegend (2 mentions) Streptavidin alkaline phosphatase, by BioLegend (2 mentions) Immunospot counter, by Cellular Technology (2 mentions) Tmb substrate, by BioLegend (2 mentions) Mutiscreen filter plates, by Merck Group (2 mentions) Mouse il 3 elisa max kit, by BioLegend (2 mentions)

2 protocols using il 17 and gm csf elispot antibody pairs

1

Cytokine Production Assay in Immunized Mice

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ELISPOT assays were performed on Mutiscreen Filter Plates (Millipore). DLN cells from immunized mice were plated at 5 × 105 - 1 × 106 cells/well in cIMDM and stimulated with 10 micromolar peptide or 1 μg/ml ConA overnight at 37°C. IL-2, IFN-γ, and IL-10 ELISPOT antibody pairs were from BD Bioscience. IL-17 and GM-CSF ELISPOT antibody pairs and streptavidin-alkaline phosphatase were from BioLegend. Plates were developed with NBT/BCIP substrate (Roche) and spots were counted on an Immunospot counter (Cellular Technology Ltd.). ELISA assays were performed on Nunc Maxisorp plates using the same antibody pairs as above but were developed with streptavidin-HRP (BioLegend) and TMB substrate (BioLegend). Standard curves for these ELISAs were generated with purified cytokines. The Mouse IL-3 ELISA MAX kit was used to measure IL-3 (BioLegend).
Lin C.C., Bradstreet T.R., Schwarzkopf E.A., Sim J., Carrero J.A., Chou C., Cook L.E., Egawa T., Taneja R., Murphy T.L., Russell J.H, & Edelson B.T. (2014). Bhlhe40 controls cytokine production by T cells and is essential for pathogenicity in autoimmune neuroinflammation. Nature communications, 5, 3551.
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2

Cytokine Production Assay in Immunized Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISPOT assays were performed on Mutiscreen Filter Plates (Millipore). DLN cells from immunized mice were plated at 5 × 105 - 1 × 106 cells/well in cIMDM and stimulated with 10 micromolar peptide or 1 μg/ml ConA overnight at 37°C. IL-2, IFN-γ, and IL-10 ELISPOT antibody pairs were from BD Bioscience. IL-17 and GM-CSF ELISPOT antibody pairs and streptavidin-alkaline phosphatase were from BioLegend. Plates were developed with NBT/BCIP substrate (Roche) and spots were counted on an Immunospot counter (Cellular Technology Ltd.). ELISA assays were performed on Nunc Maxisorp plates using the same antibody pairs as above but were developed with streptavidin-HRP (BioLegend) and TMB substrate (BioLegend). Standard curves for these ELISAs were generated with purified cytokines. The Mouse IL-3 ELISA MAX kit was used to measure IL-3 (BioLegend).
Lin C.C., Bradstreet T.R., Schwarzkopf E.A., Sim J., Carrero J.A., Chou C., Cook L.E., Egawa T., Taneja R., Murphy T.L., Russell J.H, & Edelson B.T. (2014). Bhlhe40 controls cytokine production by T cells and is essential for pathogenicity in autoimmune neuroinflammation. Nature communications, 5, 3551.
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