Heidelberg retina angiograph 2

Manufactured by Heidelberg Engineering

Sourced in Germany, United States

The Heidelberg Retina Angiograph 2 is a diagnostic imaging device designed for retinal and choroidal angiography. It captures high-resolution images of the vascular structures in the eye, allowing for detailed analysis and assessment of various eye conditions.

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Lab products found in correlation

Fluorescite, by Akorn (4 mentions) 840 hr sdois, by Bioptigen (3 mentions) Spectralis, by Heidelberg Engineering (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Ff 450plus fundus camera, by Zeiss (1 mentions) Xylazine, by Bayer (1 mentions) Ketamine, by Pfizer (1 mentions) 3d oct 1000, by Topcon (1 mentions) Spectralis sd oct, by Heidelberg Engineering (1 mentions) Daytona, by Optos (1 mentions) Clarus 500, by Zeiss (1 mentions) Growth factor reduced matrigel, by BD (1 mentions) Tx20 automatic non contact tonometer, by Canon (1 mentions) Cr 1 non mydriatic fundus camera, by Canon (1 mentions) Dri oct1 atlantis scanner, by Topcon (1 mentions) Plex elite 9000, by Zeiss (1 mentions) Sl ie slit lamp microscope, by Topcon (1 mentions) Fluorescein sodium, by Alcon (1 mentions) Rompun 2, by Bayer (1 mentions) Ff450, by Zeiss (1 mentions)

Spelling variants of this product

Heidelberg Retina Angiograph (12 citations) Heidelberg Retina Angiograph system (HRA-2; (5 citations) Heidelberg Retina Angiograph 2 (HRA 2; (5 citations) Heidelberg Retina Angiograph 2 HRA2 (HRA+OCT Spectralis: (2 citations) Heidelberg Retina Angiograph 2 scanning laser ophthalmoscope (HRA2-SLO; (2 citations)

29 protocols using heidelberg retina angiograph 2

Longitudinal Visual Outcomes of Surgical Intervention

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The preoperative and postoperative examinations included best-corrected visual acuity (BCVA) measured by Snellen chart, intraocular pressure, fundus examination by fundus photography, indirect binocular ophthalmoscopy, and OCT imaging using spectral-domain optical coherence tomography (SD-OCT, Heidelberg Retina Angiograph 2; Heidelberg Engineering, Heidelberg, Germany).
In the present study, postoperative assessments were planned at 12, 15, 18, 21, and 24 months postoperatively. Best-corrected visual acuity using a Snellen chart was converted to the logarithm of minimum angle of resolution (logMAR) for analytical purposes.

Lee P.Y., Chang Y.C., Liu P.K., Kao T.E., Wu H.J., Chen K.J., Wu K.Y., Cheng K.C, & Wu W.C. (2022). Long-Term Follow-Up of Refractory Large Macular Hole with Autologous Neurosensory Retinal Free Flap Transplantation. Journal of Ophthalmology, 2022, 1717366.

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Quantifying Choroidal Neovascularization in Mice

Cited 1 time

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FFA was performed as previously described with mice under anesthesia and pupils dilated [24 (link)]. We used a confocal, scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2; Heidelberg Engineering, Heidelberg, Germany) to evaluate CNV status. FFA images were captured at 3 to 5 min after intraperitoneal injection of 150 μL 1% (w/v) fluorescein sodium (Fluorescite; Akorn, Lake Forest, IL). FFA images taken at the same time points were analyzed using ImageJ software (National Institutes of Health, Bethesda, MD) after manual selection of the maximal leakage areas. To measure the hyperfluorescent areas, FFA images were imported into ImageJ, where the maximal border of CNV lesions was manually outlined under digital magnification. The encompassed area measurement in pixels converted to μm² using the “scale” tool in ImageJ software. To measure the hyperfluorescent intensity, the fluorescence intensity within the maximum border of each CNV lesion was calculated using ImageJ software, areas where large vessels overlapped the hyperfluorescent area was excluded. Background fluorescent intensity was measured by defining an annulus area around the CNV lesion. The net fluorescent intensity above background was calculated by subtracting the calculated background value from the CNV hyperfluorescent intensity.

Im S., Han J.W., Park E.J., Bang J.H., Shin H.J., Chang H.S., Woo K.M., Park W.J, & Park T.K. (2022). Suppression of choroidal neovascularization and epithelial-mesenchymal transition in retinal pigmented epithelium by adeno-associated virus-mediated overexpression of CCN5 in mice. PLoS ONE, 17(6), e0269937.

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Comprehensive Ophthalmic Evaluation Protocol

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All study subjects were consented before participating in the study under the protocols #AAAI9906 approved by the Institutional Review Board at Columbia University and #20130770 by the Western Institutional Review Board at the Chicago Lighthouse. The study adhered to tenets established in the Declaration of Helsinki. Complete ophthalmic examinations were provided by a retinal specialist (SHT and GAF), including slit-lamp and dilated fundus examinations. Clinical assessments (WL and FTC) were made from clinical examination notes, retinal imaging data and research questionnaires. Spectral domain-optical coherence tomography (OCT) scans and corresponding infrared reflectance fundus images were acquired using a Spectralis HRA+OCT (or HRA+OCT) (Heidelberg Engineering, Heidelberg, Germany). Fundus autofluorescence (AF) images were obtained using a confocal scanning-laser ophthalmoscope (Heidelberg Retina Angiograph 2, Heidelberg Engineering, Dossenheim, Germany). Fundus autofluorescence (AF) images were acquired by illuminating the fundus with an argon laser source (488 nm excitation) and viewing the resultant fluorescence through a band pass filter with a short wavelength cut-off at 495 nm. Colour fundus photos were obtained with a FF 450plus Fundus Camera (Carl Zeiss Meditec AG, Jena, Germany) and CR-1 Mark II Fundus Camera (Canon, Tokyo, Japan).

Zernant J., Lee W., Collison F.T., Fishman G.A., Sergeev Y.V., Schuerch K., Sparrow J.R., Tsang S.H, & Allikmets R. (2017). Frequent hypomorphic alleles account for a significant fraction of ABCA4 disease and distinguish it from age-related macular degeneration. Journal of medical genetics, 54(6), 404-412.

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In Vivo DARC Imaging of Annexin A5

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Fluorescently labelled Annexin A5 (Anx776, (Cordeiro et al., 2017 )) was given by intravitreal administration as described previously (5 μL of 0.4 μg/mL) (Cordeiro et al., 2010 (link), Galvao et al., 2013 (link), Guo et al., 2014 ). In vivo DARC imaging was performed using a modified cSLO (Heidelberg Retina Angiograph 2, Heidelberg Engineering, Dossenheim, Germany) (Cordeiro et al., 2004 (link), Maass et al., 2007 (link)) and a 55° field of view centred on the optic disc (Cordeiro et al., 2004 (link), Maass et al., 2007 (link)). No complications or intraocular side effects associated with topical treatments were recorded.

Davis B.M., Tian K., Pahlitzsch M., Brenton J., Ravindran N., Butt G., Malaguarnera G., Normando E.M., Guo L, & Cordeiro M.F. (2017). Topical Coenzyme Q10 demonstrates mitochondrial-mediated neuroprotection in a rodent model of ocular hypertension. Mitochondrion, 36, 114-123.

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Fluorescein Angiography of Retina

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FFA was performed using a scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2; Heidelberg Engineering) as previously described.⁵³ (link) In brief, the animal was anesthetized and the pupil dilated to observe the retina. FFA images were captured 3–5 min after an i.p. injection with 0.1 mL of 2% fluorescein sodium (Fluorescite; Akorn). FFA was performed at 5 and 13 days post-laser photocoagulation.

Park T.K., Lee S.H., Choi J.S., Nah S.K., Kim H.J., Park H.Y., Lee H., Lee S.H, & Park K. (2017). Adeno-Associated Viral Vector-Mediated mTOR Inhibition by Short Hairpin RNA Suppresses Laser-Induced Choroidal Neovascularization. Molecular Therapy. Nucleic Acids, 8, 26-35.

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Fundus Fluorescein Angiography of CNV

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Fundus FA was performed using a scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2; Heidelberg Engineering GmbH, Heidelberg, Germany). The presence of window defects, pooling due to PED, hemorrhage-related blockage, and the presence of hyperfluorescence that could be compatible with a choroidal neovascular membrane (CNV) were evaluated. The appearance of CNV was classified as occult (ill-defined areas of irregular staining or poorly demarcated areas of leakage in the late phase of angiogram) or classic (well-demarcated areas of intense hyperfluorescence appearing early and showing progressive leakage).

Garip R., Yasa D, & Ozkaya A. (2020). Evaluation of Clinical Findings, Optical Coherence Tomography, Fundus Fluorescein Angiography, and Indocyanine Green Angiography Imaging in Patients with Polypoidal Choroidal Vasculopathy. Beyoglu Eye Journal, 5(2), 135-141.

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Polypoidal Choroidal Vasculopathy: PDT and IVR

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We retrospectively reviewed the medical charts of consecutive patients with PCV who underwent initial PDT combined with IVR from July 2010 to August 2011 in the outpatient clinic of Tokyo University Hospital and were followed-up for at least three years. All patients underwent a standard examination that included the measurement of best-corrected visual acuity (BCVA), slit-lamp biomicroscopy, funduscopy and spectral domain optical coherence tomography (SD-OCT) (Spectralis; Heidelberg Engineering, Heidelberg, Germany) at each visit. BCVA was measured using the Landolt C chart and values were converted into logarithm of minimal angle of resolution (logMAR). Central foveal thickness (CFT) was measured by SD-OCT. The measurement of subfoveal central choroiddal thickness (CCT) was also assessed using SD-OCT images by incorporating enhanced depth imaging OCT images. All patients underwent fluorescein angiography and ICGA at the time of initial treatment unless contraindicated (Heidelberg Retina Angiograph 2; Heidelberg Engineering, Heidelberg, Germany). Greatest linear dimensions (GLD) of the lesions were measured on ICGA images (ICGA-guided GLD), which contained both polypoidal lesions and BVNs. Two investigators (K. A. and Y. N.) independently reviewed the angiography results, determined the presence of polyps and measured the GLD.

Azuma K., Okubo A., Nomura Y., Zhou H., Terao R., Hashimoto Y., Asano K.S., Azuma K., Inoue T, & Obata R. (2020). Association between pachychoroid and long-term treatment outcomes of photodynamic therapy with intravitreal ranibizumab for polypoidal choroidal vasculopathy. Scientific Reports, 10, 8337.

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Imaging Biomarkers for AMD Progression

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The study eye was analyzed for the development of CNV and GA, and for the presence and extent of RPD at baseline and at follow-up, using ICGA, AF, and NIR-R imaging. All images were acquired using a confocal scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2, Heidelberg Engineering, Heidelberg, Germany) in a 30-degree field of view at a resolution of 1536 pixels². RPD were defined as hypofluorescent dots seen in the mid to late phase of the angiogram on ICGA; hyporeflectant lesions in well-defined reticular patterns against a mildly hyperreflectant background on NIR-R; and hypoautofluorescent lesions against a background of elevated fluorescence on AF. The extent of RPD was drawn independently on each modality by 2 graders (CN and CS) using ImageJ software (Version 1.4, National Institutes of Health, Bethesda, MD, USA) and calculated as the RPD area divided by the total fundus area (in pixels²). If the 2 graders obtained an area measurement differing by >15%, arbitration through open adjudication was performed. If agreement was still not achieved, resolution was achieved by a third, expert grader (RTS). The average of the 2 observer measurements was used for statistical analysis. Images were excluded if assessment was not possible due to poor quality.

Kaszubski P.A., Ben Ami T., Saade C., Nabati C., Kumar V., Santos A.R., Silva R., Cachulo M.L., Cunha-Vaz J.G, & Theodore Smith R. (2017). Changes in Reticular Pseudodrusen Area in Eyes That Progressed from Early to Late Age-Related Macular Degeneration. International ophthalmology, 38(2), 503-511.

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Intravitreal ECFC Injection in Mice

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P13 mice were anaesthetized via intraperitoneal injection of xylazine (5 mg/kg, Bayer) and ketamine (100 mg/kg, Pfizer) and ECFCs were injected into the vitreous of the left eye at a dose of 1 × 10³ (n = 7), 1 × 10⁴ (n = 5) or 1 × 10⁵ (n = 7) cells, resuspended in 1 µl, and using a 10 µl glass syringe with a 34G needle. The right eye received an equivalent 1 µl injection of vehicle (phenol red‐free Dulbecco's modified Eagle medium, DMEM). A subset of pups received a sham injection (empty 34G needle inserted and withdrawn) into the left eye and the right eye served as an uninjected control (n = 3). At P17, fluorescein angiographs were acquired using a confocal scanning laser ophthalmoscope (cSLO, Heidelberg Retina Angiograph 2, Heidelberg Engineering, Germany) prior to sacrifice, with the eyes enucleated for immunohistochemistry.

Reid E., Guduric‐Fuchs J., O'Neill C.L., Allen L., Chambers S.E., Stitt A.W, & Medina R.J. (2017). Preclinical Evaluation and Optimization of a Cell Therapy Using Human Cord Blood‐Derived Endothelial Colony‐Forming Cells for Ischemic Retinopathies. Stem Cells Translational Medicine, 7(1), 59-67.

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Ophthalmic Evaluation and Laser Treatment

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The standard follow-up ophthalmologic examination included measurement of best corrected visual acuity (BCVA),, tonometry, and funduscopic evaluation. In addition, all patients underwent, before or after the treatment or both, fundus photography (Zeiss Retinograph Carl Zeiss, Dublin, CA), ultrawide field scanning laser ophthalmoscopy (UWF-SLO; Daytona, Optos, Dunfermline, UK), optical coherence tomography (OCT) scanning (Topcon 3D OCT-1000, Topcon Medical Systems Inc., Oakland, NJ, and Heidelberg Spectralis SD-OCT, Heidelberg Engineering, Dossenheim, Germany), and FA (Heidelberg Retina Angiograph 2 Heidelberg Engineering; Zeiss Retinograph Carl Zeiss). Argon green laser was performed using a QuadrAspheric Indirect Contact Laser Lens (Volk, Mentor, OH) in one or more sessions. The photocoagulation was targeted on the retina adjacent to the tumor, and then the laser was directed on the top of the hemangioblastoma, as suggested by Schmidt et al., to reduce the risk of retinal detachment [13 (link)]. Feeder vessels were not treated directly using laser to avoid vitreous hemorrhage. Additional individualized therapies were collected and have been described.

Murro V., Lippera M., Mucciolo D.P., Canu L., Ercolino T., De Filpo G., Giorgio D., Traficante G., Sodi A., Virgili G, & Giansanti F. (2021). Outcome and genetic analysis of patients affected by retinal capillary hemangioblastoma in von Hippel Lindau syndrome. Molecular Vision, 27, 542-554.

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