Hrp conjugated goat anti mouse

Manufactured by Jackson ImmunoResearch

Sourced in United States, Panama, United Kingdom

HRP-conjugated goat anti-mouse is a secondary antibody used for the detection of mouse primary antibodies. It is conjugated with horseradish peroxidase (HRP), allowing for colorimetric or chemiluminescent detection of target proteins in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Goat anti-mouse HRP (99 citations) HRP-conjugated goat anti-mouse IgG (H + L) (17 citations) HRP-conjugated affinipure Goat anti-mouse IgG (10 citations) HRP anti-mouse (5 citations) Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG Ab (2 citations) Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) (2 citations) Goat anti-mouse IgG antibody conjugated with HRP (2 citations) HRP-conjugated goat anti-mouse or goat anti-human IgG (H+L) (2 citations) HRP-conjugated goat anti-mouse IgG Fc-specific secondary antibodies (2 citations) HRP-conjugated goat anti-rabbit IgG and donkey anti-mouse IgG (2 citations)

32 protocols using hrp conjugated goat anti mouse

Western Blot Analysis of Protein Expression

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in Laemmli buffer and analyzed for total protein concentration as described (Karni et al. 2007 (link)). Fifty micrograms of total protein from each cell lysate was separated by SDS-PAGE and transferred onto a nitrocellulose membrane. The membranes were blocked with 5% milk and probed with specific antibodies. Bands were visualized using enhanced chemiluminescence detection. Primary antibodies are as follows: hnRNP A1 (mAb A1/55, 1:1000) (Allemand et al. 2005 (link)), hnRNP A2/B1 (1:1000, Santa Cruz), β-tubulin (1:1000, Sigma), β-catenin (1:2000, Sigma), β-actin (1:1000, Santa Cruz), T7 tag (1:5000, Novagen), phospho-MEK S217/221 (1:1000, Cell Signaling), total MEK (1:1000, Cell Signaling), phospho-ERK T202/Y204 (1:1000 Sigma), total ERK1/2 (1:1000, Cell Signaling), A-Raf (1:1000, Cell Signaling), and A-Raf short (1:500) (Rauch et al. 2011 (link)). Secondary antibodies are as follows: HRP-conjugated goat anti-mouse, goat anti-rabbit, donkey anti-goat IgG (H+L; 1:10,000 Jackson Laboratories).

Shilo A., Ben Hur V., Denichenko P., Stein I., Pikarsky E., Rauch J., Kolch W., Zender L, & Karni R. (2014). Splicing factor hnRNP A2 activates the Ras-MAPK-ERK pathway by controlling A-Raf splicing in hepatocellular carcinoma development. RNA, 20(4), 505-515.

+ Open protocol

+ Expand

Antibody Identification in Protein Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The rabbit anti-APAP-AD antibody was a kind gift from Dr. Lance Pohl (National Heart, Lung and Blood Institute, USA).^{13 (link)} The other antibodies were: CYP2E1 (#ab28146) from Abcam (Cambridge, UK), p62 (#H00008878-M01) from Abnova (Taipei, China), phospho-S6 (#4858), total S6 (#2217), phospho-JNK (#4668), and GAPDH (#2118) from Cell Signaling Technology (Danvers, MA, USA); β-actin (#a5441) from Sigma-Aldrich (St. Louis, MO, USA), total JNK (#554285) from BD Pharmingen (Franklin Lakes, NJ, USA); proliferating cell nuclear antigen (PCNA) (#SC-56) from Santa Cruz Biotechnology (Dallas, TX, USA). The rabbit anti-microtubule-associated protein 1 light chain 3 (LC3) antibody was described previously.^{22 (link)} The secondary antibodies were HRP-conjugated goat-anti-rabbit (#111-035-045), and HRP-conjugated goat-anti-mouse (#115-035-062) were from Jackson ImmunoResearch (West Glove, PA, USA). All other reagents were either from Sigma (USA) or Thermo Fisher Scientific (USA).

Sun H., Ni H.M., McCracken J.M., Akakpo J.Y., Fulte S., McKeen T., Jaeschke H., Wang H, & Ding W.X. (2021). Liver-specific deletion of mechanistic target of rapamycin does not protect against acetaminophen-induced liver injury in mice. Liver research, 5(2), 79-87.

+ Open protocol

+ Expand

Quantitative Analysis of Dystrophin and Utrophin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heart tissue from treated and untreated Fiona/dko and C57BL/10 mice were homogenized and quantified using the DC Protein Assay (Bio-Rad, 5000166). 100 μg of each homogenate sample were separated using SDS-PAGE, transferred to nitrocellulose, reversibly stained with Ponceaus S, and then incubated with primary monoclonal antibody to dystrophin (MANDYS1(3B7), 1:100, exon31/32) or utrophin (MANCHO3, 1:100, cross-reacts with mouse and human utrophin) (University of Iowa Development Studies Hybridoma Bank). HRP-conjugated goat anti-mouse (1:5,000, Jackson ImmunoResearch Laboratories, 115035146) was used as the secondary antibody, and blots were developed with ECL Western Blotting Substrate (Thermo Fisher Scientific, 32106).

Piepho A.B., Lowe J., Cumby L.R., Dorn L.E., Lake D.M., Rastogi N., Gertzen M.D., Sturgill S.L., Odom G.L., Ziolo M.T., Accornero F., Chamberlain J.S, & Rafael-Fortney J.A. (2023). Micro-dystrophin gene therapy demonstrates long-term cardiac efficacy in a severe Duchenne muscular dystrophy model. Molecular Therapy. Methods & Clinical Development, 28, 344-354.

+ Open protocol

+ Expand

Antibody Characterization for APAP Toxicity

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The rabbit anti-APAP-AD antibody was a kind gift from Dr. Lance Pohl (National Heart, Lung and Blood Institute).^{31 (link)} The other antibodies were: CYP2E1 (Abcam, #ab28146),p62 (Abnova, #H00008878-M01), phospho-eukaryotic initiation factor 4E-binding protein 1 (p-4EBP1) (Cell Signaling, #9451), total 4EBP1 (Cell Signaling, #9452), p-JNK (Cell Signaling, #4668), total JNK (BD Pharmingen, #554285), β-actin (Sigma, #a5541), and glyceralde-hyde phosphate dehydrogenase (GAPDH) (Cell Signaling, #2118). The microtubule-associated protein 1 light chain 3 (LC3) antibody was developed as described previously.³⁶ The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat-anti-rabbit (Jackson ImmunoResearch, #111–035-045), and HRP-conjugated goat-anti-mouse (Jackson ImmunoResearch, #115-035-062).

Li Y., Ni H.M., Jaeschke H, & Ding W.X. (2019). Chlorpromazine protects against acetaminophen-induced liver injury in mice by modulating autophagy and c-Jun N-terminal kinase activation. Liver research, 3(1), 65-74.

+ Open protocol

+ Expand

Immunochemical Profiling of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit and mouse IgG were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Primary antibodies used in this study include rabbit and mouse anti-FLAG (Sigma-Aldrich Co., St. Louis, MO); mouse anti-myc, chicken anti-GAPDH, and rabbit anti-D2R (Millipore, Billerica, MA); rabbit anti-zDHHC8 (Santa Cruz Biotechnology); mouse anti-GM130 (BD Biosciences, San Jose, CA); mouse anti-GFP (Life Technologies, Carlsbad, CA), and rabbit anti-phospho-p44/42 MAPK (pERK) and rabbit anti-p44/42 MAPK (total ERK) (Cell Signaling, Beverly, MA). HRP-conjugated goat anti-mouse, goat anti-rabbit and donkey anti-chicken secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA). Fluorescent secondary antibodies include Alexa Fluor 488-conjugated goat anti-rabbit, Alexa Fluor 555-conjugated goat anti-mouse, (Molecular Probes, Eugene, OR), and Cy3-conjugated donkey anti-mouse (Jackson ImmunoResearch).

Ebersole B., Petko J., Woll M., Murakami S., Sokolina K., Wong V., Stagljar I., Lüscher B, & Levenson R. (2015). Effect of C-Terminal S-Palmitoylation on D2 Dopamine Receptor Trafficking and Stability. PLoS ONE, 10(11), e0140661.

+ Open protocol

+ Expand

Antibody Characterization for Western Blotting and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for western blotting or labeling for flow cytometric analyses include: mouse anti-CD2 (OX-34 clone, Bio-Rad); mouse anti-Notch (F461.3B, DSHB); rabbit anti-V5 (Sigma); HRP-conjugated goat anti-mouse (Jackson ImmunoResearch); Alexa Fluor680 rabbit anti-mouse (Invitrogen); IRDye700 goat anti-rabbit (Rockland Immunochemicals); PE-conjugated goat anti-mouse (Life Technologies); PE-conjugated mouse anti-myc (9E10 clone, R&D systems). Western blots using fluorescent probes were visualized using the Odyssey system (LI-COR), while western blots with peroxidase conjugated probes were detected by enhanced chemiluminescence blotting substrate (Thermo Scientific) and film development.

Pandey A., Harvey B.M., Lopez M.F., Ito A., Haltiwanger R.S, & Jafar-Nejad H. (2019). Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila. Cell reports, 29(7), 2054-2066.e6.

+ Open protocol

+ Expand

Monoclonal Antibody-Based Immunoassay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal mouse anti-DNP IgE (Cat #D8406, clone SPE-7), DNP-human serum albumin (HSA), FITC-dextran (150 kDa) and BMS-345541 were from Sigma-Aldrich (St. Louis, MO). Hilyte Plus 647-conjugated goat anti-mouse IgG (Cat # AS-61057-05-H647) and Hilyte Plus 488-conjugated goat anti-mouse IgG (Cat # 61057-H488) were from Anaspec (Fremont, CA). HRP-conjugated goat anti-mouse (Cat # 115-035-166) and anti-rabbit (Cat # 115-035-003) IgG were from Jackson Immuno Research Laboratories (West Grove, PA). Monoclonal anti-HA (MMS-101R, clone 16B12) was from Covance (Princeton, NJ). Rabbit polyclonal anti SNAP23 antibodies were from Abcam (Cat # ab4114).

Klein O., Roded A., Zur N., Azouz N.P., Pasternak O., Hirschberg K., Hammel I., Roche P.A., Yatsu A., Fukuda M., Galli S.J, & Sagi-Eisenberg R. (2017). Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Scientific Reports, 7, 15315.

+ Open protocol

+ Expand

Quantitative Western Blotting for MGMT

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described [49 (link)]. The primary antibodies were mouse monoclonals against human MGMT (Santa Cruz Biotechnology, Heidelberg, Germany; catalogue #sc-56157) and against β-actin (Sigma-Aldrich; catalogue #A5441). The secondary antibody was an HRP-conjugated goat anti-mouse (Jackson ImmunoResearch, Huissen, The Netherlands; catalogue #115-035-003). The staining was revealed and images were acquired on an Amersham Imager 600 (GE Healthcare, Diegem, Belgium). The Image J software version 1.8.0 (NIH, Bethesda, MD, USA) was used for quantification.

Zampieri L.X., Sboarina M., Cacace A., Grasso D., Thabault L., Hamelin L., Vazeille T., Dumon E., Rossignol R., Frédérick R., Sonveaux E., Lefranc F, & Sonveaux P. (2021). Olaparib Is a Mitochondrial Complex I Inhibitor That Kills Temozolomide-Resistant Human Glioblastoma Cells. International Journal of Molecular Sciences, 22(21), 11938.

+ Open protocol

+ Expand

Antibody Characterization for Western Blotting and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Western Blot Analysis of LRRC8A, TTYH1, and Actin

Check if the same lab product or an alternative is used in the 5 most similar protocols

For western blotting, HEK293T, SNU-601, and TTYH1/2 double-knockout cells were lysed with lysis buffer (50 mM HEPES, 0.1% sodium deoxycholate, 1% Triton X-100, 1 mM PMSF, and 0.1% SDS containing protease inhibitor cocktail, pH 7.4). Total protein (15 μg/lane) was subjected to SDS-PAGE (8–12% gels) and transferred to PVDF membranes. The membranes were blocked using 5% non-fat milk, and then blotted with the appropriate antibodies: Anti-LRRC8A (Cell Signaling Technology, Inc., Danvers, MA, USA, #24979), anti-TTYH1 (CUSABIO technology LLC, Houston, TX, USA, CSB-PA867139LA01HU), and anti-actin (Sigma, St. Louis, MO, USA, A5441). The membranes were then washed and incubated with HRP-conjugated goat anti-mouse (Jackson ImmunoResearch, #115-035-166), goat anti-rabbit (Jackson ImmunoResearch, #111-035-144), or rabbit anti-goat IgG (Jackson ImmunoResearch Lab, #305-035-045), followed by washing and detection of immunoreactivity using ECL (Thermo, #34095).

Bae Y., Kim A., Cho C.H., Kim D., Jung H.G., Kim S.S., Yoo J., Park J.Y, & Hwang E.M. (2019). TTYH1 and TTYH2 Serve as LRRC8A-Independent Volume-Regulated Anion Channels in Cancer Cells. Cells, 8(6), 562.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!