Polyclonal rabbit anti α cat c3236

The following primary antibodies were used: polyclonal rabbit anti-α-cat (C3236, Cell Signaling), hybridoma mouse anti-α-catenin (5B11, (Daugherty et al., 2014 (link))), rabbit anti-GAPDH (Cat# sc-25778, Santa Cruz, now discontinued), polyclonal rabbit anti-GFP (A11122, Invitrogen) and Phalloidin-488 or -568 (A12379, Invitrogen). Secondary antibodies for Western blotting included HRP-conjugated goat anti-mouse and -rabbit antibodies (Bio-Rad), or fluorescently labeled donkey anti-mouse and -rabbit antibodies (680RD or 800RD, LiCor Biosciences). Secondary antibodies for immunofluorescence included IgG Alexa Fluor 488 or 568-conjugated goat anti-mouse or -rabbit antibodies (Invitrogen).

The following primary antibodies were used: polyclonal rabbit anti–αCat (C3236; Cell Signaling), hybridoma mouse anti–αCat (5B11; Daugherty et al., 2014 (link)), monoclonal mouse anti–β-catenin (610154; BD Biosciences), polyclonal rabbit anti–β-catenin (06-734; EMD Millipore), monoclonal mouse anti–E-cadherin (610182; BD Biosciences), monoclonal mouse anti–E-cadherin (HECD1, 13-1700; Takara), polyclonal rabbit anti-GAPDH (FL-335, sc-25778; Santa Cruz), anti–p34-Arc/ARPC2 (07-227; EMD Millipore), monoclonal mouse anti-GAPDH (9484; Abcam), hybridoma mouse anti-tubulin (DM1A, T9026; Sigma-Aldrich), polyclonal rabbit anti-mCherry (5993; BioVision), and Alexa Fluor 488 or 568 phalloidin (A12379; Invitrogen). Secondary antibodies for Western blotting included HRP-conjugated goat anti–mouse and anti–rabbit antibodies (Bio-Rad) or fluorescently labeled donkey anti–mouse and anti–rabbit antibodies (680RD or 800RD; LiCor Biosciences). Secondary antibodies for immunofluorescence included IgG Alexa Fluor 488– or 568–conjugated goat anti–mouse or anti–rabbit antibodies (Invitrogen).