Log In Sign up
The largest database of trusted experimental protocols

δ9 thc

Manufactured by Cerilliant
Visit Supplier
Sourced in United States

Δ9-THC is a reference material used for the identification and quantification of Δ9-THC in analytical testing. It is a highly pure chemical standard that can be used to calibrate analytical instruments and validate testing methods.

Automatically generated - may contain errors

Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (3 mentions) Methanol, by Thermo Fisher Scientific (3 mentions) Cannabidivarin, by Cerilliant (3 mentions) Cannabichromene, by Cerilliant (3 mentions) Lc ms grade water, by Thermo Fisher Scientific (2 mentions) Delta 8 tetrahydrocannabinol δ 8 thc, by Cerilliant (2 mentions) Delta 9 tetrahydrocannabinol d3 δ 9 thc d3, by Cerilliant (2 mentions) Cannabigerol, by Cerilliant (2 mentions) Cannabidiolic acid, by Cerilliant (2 mentions) Cannabigerolic acid, by Cerilliant (2 mentions) Tetrahydrocannabivarin, by Cerilliant (2 mentions) Cbd d3, by Cerilliant (2 mentions) Cbn d3, by Cerilliant (2 mentions) 11 oh thc, by Cerilliant (2 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) Lc ms grade water, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) δ8 thc, by Cerilliant (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) N hexadecane, by Merck Group (1 mentions)

Spelling variants of this product

Δ9-THC-d3 (3 citations)

7 protocols using δ9 thc

1

Quantitative Analysis of Cannabinoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available standards (purity >98%) for cannabichromene (CBC), cannabicyclol (CBL), CBD, cannabidiolic acid (CBDA), cannabidivarin (CBDV), cannabigerol (CBG), cannabigerolic acid (CBGA), cannabinol (CBN), delta-8-tetrahydrocannabinol (Δ-8-THC), Δ-9-THC, THCA, and tetrahydrocannabivarin (THCV) were obtained from Cerilliant (Round Rock, TX, USA). Additionally, deuterated internal standards (purity >98%) (IS) delta-9-tetrahydrocannabinol-D3 (Δ-9-THC-D3) and 11-nor-9-carboxy-Δ-9-THC-D9 (11-nor-9-COOH-Δ-9-THC-D9) were also obtained from Cerilliant (Round Rock, TX, USA). LC-MS grade water, methanol, and formic acid were sourced from Fisher Scientific (Fair Lawn, NJ, USA). Commercially available hops, Humulus lupulus, were obtained from BioKoma (Old Mill Creek, IL, USA).
Berthold E.C., Yang R., Sharma A., Kamble S.H., Kanumuri S.R., King T.I., Popa R., Freeman J.H., Brym Z.T., Avery B.A, & McCurdy C.R. (2020). Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids. Journal of Cannabis Research, 2, 42.
+ Open protocol
+ Expand
2

Certified Cannabinoid Standards Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Certified reference materials for CBDA, CBD, THCA, Δ9-THC, Δ8-THC, CBN, CBC, CBG, CBGA, cannabidivarin (CBDV), and tetrahydrocannabivarin (THCV) in 1.0 mg/mL concentrations were obtained from Cerilliant Corporation (Round Rock, Texas). Bulk quantities of Δ9-THC in ethanol (100 mg/mL) and THCA, CBN, CBD, and CBDA in crystalline powder form were obtained from Lipomed, Inc (Cambridge, MA). Epidiolex was provided by Greenwich Biosciences (Carlsbad, CA). HPLC-grade ethyl alcohol, LC/MS grade-water, LC/MS-grade acetonitrile, and LC/MS-grade acetic acid were purchased from Millipore-Sigma (Billerica, MA). For quantification of toxic elements, multi-element stock solutions were obtained from Inorganic Ventures (Christiansburg, VA). Nitric acid (HNO3), hydrogen peroxide (H2O2), and hydrochloric acid (HCl) used for elemental analysis were double-distilled Optima grade purchased from Fisher Scientific (Fair Lawn, NJ).
Dubrow G.A., Pawar R.S., Srigley C., Fong Sam J., Talavera C., Parker C.H, & Noonan G.O. (2021). A survey of cannabinoids and toxic elements in hemp-derived products from the United States marketplace. Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems, 97, 103800.
+ Open protocol
+ Expand
3

Quantification of Cannabinoids and Polyamines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Deuterated water (D2O) 99.97 atom% of deuterium and 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt (TSP) were purchased from Euriso-Top (Saclay, France). HPLC-PDA chemical standards, n-hexadecane, HIS, SPM, SPD, PUT, (β-PEA), CAD, TYM and 1,7-diaminoheptane were purchased from Sigma-Aldrich (Milan, Italy). Methanol (HPLC-grade), chloroform (HPLC-grade), ethanol (analytical-grade), perchloric acid (70%), acetone (analytical-grade), acetonitrile (HPLC-grade) were obtained from Carlo Erba Reagenti (Milan, Italy). Double-distilled water was obtained using a Millipore Milli-Q Plus water treatment system (Millipore Bedford Corp., Bedford, MA, USA). Sodium carbonate (Na2CO3; 99.999% purity), Folin-Ciocalteu’s phenol reagent, tannic acid (Ph. Eur. purity) and aluminium chloride hexahydrate (AlCl3 × 6 H2O; Ph. Eur. purity) were purchased from Merck (Darmstadt, Germany).
Cannabinoids reference standards in methanol CBDV (1 mg/mL), CBG (1 mg/mL), CBD (1 mg/mL), CBN (1 mg/mL), (–)-Δ9-THC (0.1 mg/mL) and CBC (1 mg/mL) with purity ≥99%, were purchased from Cerilliant Corporation (Round Rock, TX, USA). For mobile phase, gradient grade water (H2O) and acetonitrile (ACN) were purchased from Sigma Aldrich (St. Louis, MO, USA) as well as trifluoroacetic acid (TFA) and analytical grade ethanol used for the extraction procedure. All solvents were further filtered on a 0.2 μm filter.
Ingallina C., Sobolev A.P., Circi S., Spano M., Fraschetti C., Filippi A., Di Sotto A., Di Giacomo S., Mazzoccanti G., Gasparrini F., Quaglio D., Campiglia E., Carradori S., Locatelli M., Vinci G., Rapa M., Ciano S., Giusti A.M., Botta B., Ghirga F., Capitani D, & Mannina L. (2020). Cannabis sativa L. Inflorescences from Monoecious Cultivars Grown in Central Italy: An Untargeted Chemical Characterization from Early Flowering to Ripening. Molecules, 25(8), 1908.
+ Open protocol
+ Expand
4

Optimized Quantification of Cannabinoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optima-grade ammonium formate, formic acid, and methanol were purchased from Fisher Scientific (Hanover Park, IL, USA). Ultrapure Type 1 water was generated in-house as needed from a Millipore Direct-Q3 system. Certified reference materials for a quality assurance test mix containing amitriptyline, diazepam, fluoxetine, methadone, nicotine, nordiazepam, norfluoxetine, nortriptyline, paroxetine, and trazodone were acquired from Cerilliant (Round Rock, TX, USA), as were CBD, CBD-d3, Δ9-THC, Δ9-THC-d3, cannabigerol (CBG), cannabidivarin (CBDV), cannabichromene (CBC), cannabinol (CBN), and CBN-d3. Δ9-Tetrahydrocannabinolic acid Δ9-THCA-A), Δ8-THC, and cannabidiolic acid (CBDA) were purchased from Cayman Chemical (Ann Arbor, MI, USA).
Holt A.K., Karin K.N., Butler S.N., Ferreira A.R., Krotulski A.J., Poklis J.L, & Peace M.R. (2022). Cannabinoid-based vaping products and supplement formulations reported by consumers to precipitate adverse effects. Drug testing and analysis.
+ Open protocol
+ Expand
5

Quantitative Analysis of Cannabinoids

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Reference standards (Δ9-THC, 11-OH-THC, CBD, and cannabinol [CBN]) and isotopically labeled internal standards (THC-d3, 11-OH-THC-d3, CBD-d3, CBN-d3) were purchased from Cerilliant (Round Rock, TX). BondElut® certify (Varian, Lake Forest, CA) C-18 cartridges were used for solid-phase extraction. HPLC grade water, 0.1% formic acid in acetonitrile (ACN), and methanol (MeOH) were purchased from Fisher Scientific. Reagent grade ammonium formate and pure sodium hydroxide pellets were purchased from Sigma-Aldrich.
We followed the Wei et al saponification-solid-phase extraction method for sample clean up and extraction with minor modifications.15 (link)Chromatographic separation was achieved on reverse-phase C-18 column (MAC-MOD Ace 5 C-18, 15cm · 2.1mm). The mobile phase consisted of 5 mM ammonium formate in 0.05% formic acid (Solvent A) and ACN in 0.1% formic acid (Solvent B). A gradient elution was performed over 15 minutes at a flow rate of 200 μL/min. Retention times for 11-OH-THC, CBD, THC were 9.35, 10.62, and 11.70 minutes in positive ion mode, respectively. Quantitation of THC, 11-OH-THC, and CBD and isotopically labeled internal standards was achieved on a triple quadrupole mass spectrometer (API-4000, AB Sciex) in positive ion mode. CBN and CBN-d3 were detected in negative ion mode.
Bertrand K.A., Hanan N.J., Honerkamp-Smith G., Best B.M, & Chambers C.D. (2018). Marijuana Use by Breastfeeding Mothers and Cannabinoid Concentrations in Breast Milk. Pediatrics, 142(3), e20181076.
+ Open protocol
+ Expand
6

Cannabinoid Standards for LC-MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available standards for delta-8-tetrahydrocannabinol (Δ8THC) (100 μg/mL), Δ9THC (100 μg/mL), 11-OH-THC (100 μg/mL), 11-COOH-THC (100 μg/mL), and delta-9-tetrahydrocannabinol-d3 (Δ9THC-d3) [(100 μg/mL); internal standard (IS)] stock solutions were obtained from Cerilliant (Round Rock, TX, United States). Liquid chromatography-mass spectrometry (LC-MS) grade water, methanol, and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, United States).
Gazarov E.A., Zequeira S., Senetra A.S., Howard J., Sharma A., McCurdy C.R., Lewis J., Bizon J.L, & Setlow B. (2023). Pharmacokinetics of delta-9-tetrahydrocannabinol following acute cannabis smoke exposure in mice; effects of sex, age, and strain. Frontiers in Pharmacology, 14, 1227220.
+ Open protocol
+ Expand
7

Cannabinoid Standards Procurement and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cannabinol (CBN), cannabichromenic acid (CBCA), cannabichromene (CBC), cannabigerolic acid (CBGA), CBG, cannabidiolic acid (CBDA), CBD, Δ9tetrahydrocannabinoic acid (THCA), and Δ9-THC were purchased from Cerilliant (Cerilliant Corporation, Round Rock, TX). Cannabidivarinic acid (CBDVA), cannabidivarin (CBDV), tetrahydrocannabivarin (THCV), and Δ8-THC were purchased from Restek (Bellefonte, PA). All cannabinoid standards are CRM standards, at 1000 μg/mL in methanol.
Eyal A.M., Hen-Shoval D., Schlesinger D., Berneman-Zeitouni D, & Raz N. (2023). Inconsistency in the Composition of the Smoke of a Cannabis Cigarette as Smoking Progresses: Results, Mechanism, and Implications. Cannabis and cannabinoid research.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!