Based on the manufacturer's operating steps, the apoptosis of the upper lobe of the lung tissues was detected using a TUNEL fluorescence FITC kit (Roche, USA). After TUNEL staining, the nuclear was stained using DAPI (1 : 100, Beyotime, China). Finally, the stained sections are photographed by fluorescence microscope and counted apoptotic cells.
Tunel fluorescence fitc kit
Manufactured by Roche
Sourced in United States, Germany
The TUNEL fluorescence FITC kit is a laboratory tool used for the detection and quantification of apoptosis, a form of programmed cell death. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay, which labels the fragmented DNA present in apoptotic cells with a fluorescent FITC (Fluorescein isothiocyanate) dye. This allows for the visualization and analysis of apoptotic cells through fluorescence microscopy or flow cytometry.
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Lab products found in correlation
Ix73 fluorescence microscope, by Olympus (5 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (5 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (3 mentions)
Fluoview 1000, by Olympus (3 mentions)
Fv1000, by Olympus (3 mentions)
Simvastatin, by Merck Group (1 mentions)
Tunel reaction mixture, by Merck Group (1 mentions)
Lsm 510 confocal microscope, by Zeiss (1 mentions)
Fv1000 confocal laser scanning microscope, by Olympus (1 mentions)
Fluorescence microscopy, by Nikon (1 mentions)
Fv300, by Olympus (1 mentions)
Fluorescence microscope, by Nikon (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)
Confocal laser scanning microscope, by Olympus (1 mentions)
18 protocols using tunel fluorescence fitc kit
1
Apoptosis Detection in Lung Tissues
2
Quantifying Apoptosis in Myocardial Tissue
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The TUNEL assay was used for the analysis of apoptosis of myocardial tissue in experimental mice at 37°C following simvastatin treatment (10 mg/kg/day; Sigma-Aldrich; Merck KGaA) or an equivalent dose of PBS. Procedures were performed as previously described (27 (link)). Briefly, 4-µm tissue sections were fixed with 4% paraformaldehyde at 37°C followed by permeabilization with 0.1% Triton X-100 for 1 h at 37°C. Subsequently, tissue sections or myocardial cells (1×106) were stained with TUNEL reaction mixture (Sigma-Aldrich; Merck KGaA) at 37°C for 2 h. Tissue sections and myocardial cells were washed 3 times in TBS-Tween-20. TUNEL assays were conducted using a TUNEL fluorescence FITC kit (Roche Diagnostics, Indianapolis, IN, USA) according to the manufacturer's instructions. Samples were mounted with neutral gum. Images in 6 fields of view (magnification, ×400) were captured using a Zeiss LSM 510 confocal microscope (Zeiss AG, Oberkochen, Germany) at a wavelength of 488 nm.
3
Detecting Apoptosis in Post-MI Heart
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Apoptosis of mice heart was detected by staining ventricular specimens (ischemic border zone) three days post-MI and that of neonatal rat cardiomyocytes was determined with In situ Cell Death Detection Kit (TUNEL fluorescence FITC kit, Roche, Penzberg, Germany) according to the manufacturer’s instruction. After TUNEL staining, the ventricular specimens or cardiomyocytes were immerged into the DAPI (Sigma, St. Louis, MO, USA) solution to stain nuclei and apoptotic cells. Fluorescence staining was viewed under a laser scanning confocal microscope (Olympus, Fluoview1000; Tokyo, Japan).
4
TUNEL Assay for MCF-7 Apoptosis
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According to the manufacturer's instructions, apoptosis of MCF-7 detected using the in situ cell death detection kit (TUNEL fluorescence FITC kit, Roche, USA). After TUNEL staining, MCF-7 cells stained using DAPI (Sigma Aldrich) and observed using laser scanning confocal microscopy (Olympus, Fluoview1000, Tokyo, Japan). The number of apoptotic cells expressed as a percentage of the total cells counted.
5
Apoptosis Detection in Cardiomyocytes
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According to the manufacturer’s instructions, the apoptosis of H9c2 cells, NRVMs, and the LV was detected using terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) fluorescence FITC kit (Roche, USA) by the TUNEL staining method. After TUNEL staining, 4’,6-diamidino-2-phenylindole (1:100, Beyotime Biotechnology, China) was used to stain nuclei solution. Fluorescence staining was observed with a Confocal Laser Scanning Microscope (FV1000, Olympus, Japan). The apoptotic rate was calculated as TUNEL-positive cells per field.
6
Apoptosis Detection in Cardiomyocytes and Ventricular Tissue
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The TdT-mediated dUTP nick end labeling (TUNEL) staining was employed to detect the apoptosis in NRVCs and left ventricles (border zones) using a TUNEL fluorescence FITC kit (Roche, USA) according to the manufacturer’s instruction. After TUNEL staining, the cardiomyocytes or the ventricular specimens were immerged into DAPI (1:30, Beyotime Biotechnology, China) solution to stain nuclei. Fluorescence staining was viewed by a Laser Scanning Confocal Microscope (FV1000, Olympus, Japan). The apoptotic rate was calculated as TUNEL-positive cells per field.
7
Apoptosis Detection via TUNEL Assay
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We used the in situ Cell Death Detection Kit (TUNEL fluorescence FITC kit, Roche, Germany) detect apoptotic. We used DAPI to stain nuclei. We used IX73 fluorescence microscope (Olympus, Valley, PA) to analyze fluorescence staining. We used Image-J to count the Total cells and TUNEL positive cells numbers.
8
Quantifying Apoptosis by TUNEL Assay
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We used the in situ Cell Death Detection Kit (TUNEL fluorescence FITC kit, Roche, Germany) detect apoptotic. We used the DAPI to stain nuclei. We used the IX73 fluorescence microscope (Olympus, Valley, PA) to analyze fluorescence staining. We used the Image-J to count the Total cells and TUNEL positive cells numbers.
9
TUNEL Apoptosis Quantification in MI
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Apoptotic cells were labeled using the TUNEL fluorescence FITC kit (Roche, Germany), as following the manufacturer's instructions. Following TUNEL staining, living, and apoptotic cell nuclei were stained by immersing heart sections (7d after MI) in Hoechst 33342 solution. Fluorescent staining was observed using a laser scanning confocal microscope (Olympus, Fluoview1000, Japan). Image-Pro Plus was used to count TUNEL-positive cells and total cell numbers.
10
Apoptosis Detection in Tissues
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Tissues were fixed in 4% paraformaldehyde tissue, and TUNEL fluorescence FITC kit (Roche, USA) was utilized for cell apoptosis assay as previous report [14 (link)]. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining was used to detect DNA fragmentation (green fluorescence) with the nuclei stained with DAPI (blue fluorescence). Images were captured through fluorescence microscopy (Nikon Co., Japan).
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