Animals were tracked and recorded with SMART Junior software (Panlab, Spain). Percentage of preference was calculated as (time exploring relocated object or new object)/(total time exploring both objects) × 100.
Smart junior software
Manufactured by Harvard Apparatus
Sourced in Spain
SMART Junior software is a data acquisition and analysis platform designed for use with Harvard Apparatus research instruments. It provides real-time data collection, display, and analysis capabilities to support various experimental protocols.
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Lab products found in correlation
11 protocols using smart junior software
1
Object Exploration Behavior Analysis
2
Evaluating Social Novelty Preference in Mice
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The apparatus consisted of three interconnected lined compartments with open doors. Subject mice were habituated to the central compartment with closed doors for 5 min. After the habituation phase, the doors were removed and subjects were tested in the sociability task, and 10 min later the social memory task was performed to evaluate preference for social novelty for 10 additional minutes. Thus, the sociability task consisted of placing the subject mice in the middle chamber and allowing them to explore for 5 min. The doorways into the two side chambers were obstructed by plastic boxes during this habituation phase. After the habituation period, the plastic boxes were removed and an unfamiliar mouse (stranger 1) was placed in one of the side chambers enclosed in a small, round wire cage that allowed nose contact between the bars but prevented fighting. In the social memory task, a second, unfamiliar mouse was placed in the chamber that had been empty during the first 10 min session (or sociability task). This second stranger (stranger 2) was also enclosed in an identical small wire cage. The test mouse had a choice between the first, already-investigated unfamiliar mouse (stranger 1) and the novel unfamiliar mouse (stranger 2). Time sniffing/exploring each small cage were measured using the SMART junior software (Panlab).
3
Elevated Plus-Maze Exploration Behavior
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The plus-maze height of 50 cm included a central square, two open arms, and two closed arms. The two closed arms were 30 cm in length and 5 cm in width and enclosed by walls with a height of 15 cm. Open arms had no walls. Mice were placed in the central square facing one of the open arms and allowed to explore individually for 5 min. The total time spent in open arms was calculated using the Smart Junior software (version 3.0; Panlab, Spain).
4
Immobility Duration in Tail-Suspended Mice
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The mice were fixed with the tip of their tail on a horizontal scaffold at the height of 50 cm with the head down. Next, the duration of immobility was recorded for 6 min by the Smart Junior software (version 3.0; Panlab, Spain).
5
Pharmacological Modulation of Locomotor Activity
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Locomotor activity was measured in an open field and recorded with a video caption system. Animals were placed into the center of an open field arena (a white square arena measuring 40 × 40 × 40 cm in length, width, and height respectively), and left to habituate for 25 min. Dim light intensity was 60 lux throughout the arena. After this period, mice received an intraperitoneal injection of vehicle (0.9% NaCl), R-(-)-apomorphine (0.5 mg/kg; SIGMA A4393), or D-amphetamine sulfate (3 mg/kg; TOCRIS 2813) as previously described (Pineda et al., 2005 (link); Sherrill et al., 2013 (link)) and their behavior was recorded over the following 45 min. The same mice were evaluated with both dopaminergic stimulants. Data caption were recorded at 1 min intervals using the SMART Junior Software (Panlab) and the distance covered/pathlength (in cm) was analyzed.
6
Open-Field Locomotor Activity Analysis
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The open-field box was 40 ×40 ×40 cm. The total distance traveled in five min was analyzed using Smart Junior software (version 3.0; Panlab, Spain).
7
Circular Agitation and Inverted Exposure
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For this test, mice were placed on a horizontal cage lid which was then agitated circularly three times and next turned upside down for 60 s 20 cm above a housing cage.
In all tasks, animal tracking and recording were performed using the automated SMART junior software (Panlab, Spain).
In all tasks, animal tracking and recording were performed using the automated SMART junior software (Panlab, Spain).
8
Spatial and Recognition Memory in R6/1 Mice
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NOLT and NORT were used to analyze hippocampal‐dependent spatial long term and recognition memory, respectively, in wild‐type and R6/1 mice at 13 weeks of age as previously described (Garcia‐Forn et al, 2018 ). In each of the tests, the object preference was measured as the time exploring each object × 100/time exploring both objects. The arena and the objects were rigorously cleaned between animal trials to avoid odors. Animals were tracked with SMART Junior software from Panlab (Barcelona, Spain). Days, in which treatment and tests were coincident, the mice were allowed to recover for 1 h after the treatment before starting any task.
9
Novel Object Recognition in Aged Mice
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Novel object recognition testing (NORT) was performed in a 30 × 44 cm arena with opaque sides, with a 24‐h retention time to test long‐term memory (Puigdellívol et al., 2021 (link)). Briefly, 4‐ and 17‐month‐old mice were first habituated to the arena in the absence of objects on two consecutive days (15 min/day), when spontaneous locomotor activity (total distance traveled) and anxiety/motivation (distance traveled in periphery versus center of the open field) were measured. On the third day, two similar objects were presented for 10 min (A and A' objects). Twenty‐four hours later, the same animals were retested for 5 min in the arena with a familiar (A) and a new (B) object. The object preference was measured as the time exploring each object × 100/time exploring both objects. Animals were tracked and recorded with SMART Junior software (Panlab). Objects and arena were cleaned thoroughly with 70% ethanol and dried after each trial to eliminate odor cues. Experimenter was blinded to the genotype of the individual animals.
10
Novel Object Location Test in Mice
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For the novel object location test (NOL), an open-top arena (45 × 45 × 45 cm) with visual cues surrounding the apparatus was used. Mice were first habituated to the arena (1 day, 30 min). We considered this first exposition to the open arena as an open field paradigm. We monitored total traveled distance, time spent in the center of the arena and parallel index as measures of locomotor activity, anxiogenic behavior and spatial navigation strategies respectively. On day 2, two identical objects (A1 and A2) were placed in the arena and explored for 10 min. Twenty-four hour later (Day 3), one object was moved from its original location to the diagonally opposite corner and mice were allowed to explore the arena for 5 min. The object preference was measured as the time exploring each object × 100/time exploring both objects. Behavioral data was processed and analyzed using the Smart Junior software (Panlab, Spain).
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