Human CCA cell lines, including H1 (OCUCh-LM1-H1)[9 (link)], HuCCT1[10 (link)], SSP-25[11 (link)], and ETK1[12 (link)] cell lines were kindly provided by Dr. Jack Wands. TFK-1 and RBE were purchased from RIKEN Cell Bank (Tsukuba, Japan). The non-malignant human bile duct cells hBD was purchased from Celprogen Inc (Torrance, CA, United States, Catalog Number: 36755-12). CCA cell lines were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS; GibcoTM, Thermo Scientific, Waltham, MA, United States; Cat# 10099141C), 100 μg/mL streptomycin, and 100 U/mL penicillin (HyClone™, Logan, UT, United States). The hBD cell line was grown in DMEM containing 10% FBS, 100 μg/mL strep. All cell lines were authenticated by STR and were negative for contamination of mycoplasma recently. Arcyriaflavin A, Flavopiridol, Roscovitine, and γ-secretase inhibitor, DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycinet-butyl ester) were all purchased from Cayman Chemical (Ann Arbor, MI, United States).
Tfk 1
Manufactured by RIKEN Cell Bank
Sourced in Japan
The TFK-1 is a laboratory equipment used for the cultivation and maintenance of cell lines. It provides a controlled environment for the growth and propagation of various cell types. The core function of the TFK-1 is to maintain optimal conditions, such as temperature, humidity, and gas composition, to support the proliferation and viability of cultured cells.
Automatically generated - may contain errors
Lab products found in correlation
Ssp 25, by RIKEN Cell Bank (3 mentions)
Tgbc 24tkb, by RIKEN Cell Bank (2 mentions)
Hucct1, by RIKEN Cell Bank (2 mentions)
Flavopiridol, by Cayman Chemical (1 mentions)
Arcyriaflavin a, by Cayman Chemical (1 mentions)
Roscovitine, by Cayman Chemical (1 mentions)
Gibcotm, by Thermo Fisher Scientific (1 mentions)
Snu1196, by Korean Cell Line Bank (1 mentions)
Snu 308, by Korean Cell Line Bank (1 mentions)
Cisplatin, by Adooq (1 mentions)
Kku 214, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hucca 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku156, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku 055, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku 100, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Mmnk 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kku 213, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hucct1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
5 protocols using tfk 1
1
Characterization of Human CCA Cell Lines
2
Establishment of Gemcitabine-Resistant BTC Cell Lines
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BTC cell lines (RBE, HuCCT1, SSP-25, TGBC-24TKB, and TFK-1) were purchased from the RIKEN Cell Bank (Ibaraki, Japan). BTC cell lines (SNU-308 and SNU-1196) were purchased from the Korean cell line bank (Seoul, Korea). RBE, HuCCT1, SSP-25, TFK-1 SNU-308, and SNU-1196 cell lines were grown in RPMI (Roswell Park Memorial Institute) medium supplemented with 10% fetal bovine serum (FBS), penicillin-streptomycin, L-glutamine, and sodium pyruvate. TGBC-24TKB cells were grown in DMEM supplemented with 10% FBS and penicillin–streptomycin. The gemcitabine-resistant cells (SNU-1196-GR and SSP-25-GR cells) were generated in the cell culture system. The cells were treated with a half-maximal inhibitory concentration (IC50) dose of gemcitabine for one month (20 nM for SSP-25 cells and 10 nM for SNU-1196 cells). After one month, the media were replaced with the fresh media containing an IC90 dose of gemcitabine (250 nM for SSP-25 cells and 60 nM for SNU-1196 cells) for another month. After two months, the live cells were identified as gemcitabine-resistant cells. All the cell lines used in this study were tested for mycoplasma contamination and authenticated by STR (short tandem repeat) method. AZD0156 (ATM inhibitor), gemcitabine, and cisplatin were purchased from AdooQ BioScience (Irvine, CA, USA).
3
Bile Duct Cancer Cell Migration Assay
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KKU-213, KKU-214, KKU-156, KKU-100, KKU-055, HuCCA-1, HuCCT1 and MMNK-1 were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank, Japan. RBE, SSP-25, and TFK-1 were obtained from RIKEN Cell bank, Japan. KKK-D068, KKK-D131, KKK-D138 were obtained from Khon Kaen University, Thailand18 (link). MCF7, HCT116, HeLa, A549 were purchased from ATCC. All cells were maintained at 37 °C in a humidified 5% CO2 atmosphere using growth media that were recommended by original cell sources (see complete details in Supplementary Table S1 ). The cell line panel consists of cancer of bile duct, which is known to be relatively migrative. Other cell lines with similar migratory property also include breast cancer (MCF7), lung cancer (A549), colorectal cancer (HCT116), and cervical cancer (HeLa). However, to test our novel method we selected a set of bile duct carcinoma cell lines known to be migrative as our model.
4
Cholangiocarcinoma Cell Lines with KRAS Mutations
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Six cholangiocarcinoma cell lines used in this study (HuCCT1, SSP-25, RBE, YSCCC, TGBC-24TKB and TFK-1) were purchased from RIKEN Cell Bank (Tsukuba, Japan). The HuCCT1, SSP-25 and RBE cell lines had a KRAS mutation, whereas the remaining three cell lines were KRAS wild type. All in vitro studies were performed at least in triplicate.
5
Metformin Effects on Human Cholangiocarcinoma Cells
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Reagents. Metformin (1,1-dimethylbiguanide monohydrochloride) was purchased from Astellas Pharma (Tokyo, Japan); all other chemicals were obtained from Sigma Chemical (Tokyo, Japan).
Cell culture. The two human CCA cell lines (TFK-1 and HuCCT-1) were included in this study. TFK-1 was obtained from the Riken Cell Bank (Tsukuba, Japan), and HuCCT-1 was obtained from the Japanese Cancer Research Resources Bank (Osaka, Japan).
All cells were grown in RPMI-1640 (Gibco-Invitrogen, Carlsbad, CA, uSA), supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (9,100 mg/l; Invitrogen) at 37˚C in a humidified atmosphere that contained 5% CO 2 .
Cell culture. The two human CCA cell lines (TFK-1 and HuCCT-1) were included in this study. TFK-1 was obtained from the Riken Cell Bank (Tsukuba, Japan), and HuCCT-1 was obtained from the Japanese Cancer Research Resources Bank (Osaka, Japan).
All cells were grown in RPMI-1640 (Gibco-Invitrogen, Carlsbad, CA, uSA), supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (9,100 mg/l; Invitrogen) at 37˚C in a humidified atmosphere that contained 5% CO 2 .
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