The following mycotoxin standards and metabolites were purchased from Biopure (Tulln, Austria): fumonisin B1, fumonisin B2, fumonisin B3, ochratoxin A, aflatoxin B1, nivalenol, diacetoxyscirpenol, and zearalenone. Acetonitrile (ACN), water and methanol (MeOH) were purchased from Biosolve (Valkenswaard, Netherlands) and formic acid (FA) from Merck (Amsterdam, Netherlands). The working solutions were prepared as follows: fumonisins solution containing FB1, B2, and B3 (each at 5 μg/mL) was diluted in acetonitrile/water (50/50 v/v) and stored 4°C; aflatoxin B1 (at 1 μg/mL), ochratoxin A solution (1 μg/mL), and the other mycotoxins solution containing nivalenol, deoxynivalenol, zearalenone (each at 10 μg/mL) were diluted in acetonitrile and were stored −20°C.
Diacetoxyscirpenol
Manufactured by Romer Labs
Sourced in Austria
Diacetoxyscirpenol is a laboratory standard used for analytical purposes. It is a metabolite produced by certain fungi and is commonly used as a reference compound in research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
Nivalenol, by Romer Labs (4 mentions)
Ochratoxin a, by Romer Labs (4 mentions)
Zearalenone, by Romer Labs (3 mentions)
Deoxynivalenol, by Romer Labs (2 mentions)
T 2 toxin, by Romer Labs (2 mentions)
Ht 2 toxin, by Romer Labs (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
Formic acid, by Merck Group (1 mentions)
13c don, by Romer Labs (1 mentions)
13c zen, by Romer Labs (1 mentions)
Acetonitrile acn gradient grade, by Merck Group (1 mentions)
Celite 545, by Merck Group (1 mentions)
Simplicity 185, by Merck Group (1 mentions)
3 acetyldeoxynivalenol, by Romer Labs (1 mentions)
Milli q system, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Patulin, by Romer Labs (1 mentions)
Deoxynivalenol, by Merck Group (1 mentions)
4 protocols using diacetoxyscirpenol
1
Mycotoxin Standards and Metabolites Preparation
2
Multi-Mycotoxin Analysis Protocol
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Ochratoxin A, deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS), zearalenone (ZEN), 13C-DON, 13C-T-2, 13C-HT-2 toxins, and 13C-ZEN were purchased from Biopure (Tulln, Austria). Ammonium acetate, Celite® 545, acetic acid, acetonitrile (ACN; gradient grade), and methanol (MeOH; MS grade) were supplied by Merck-Millipore (Darmstadt, Germany). The Simplicity 185 (Millipore, Bedford, MA, USA) water purification system was used for the deionized water production.
3
Mycotoxin Standard Solutions for Analysis
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Mycotoxin standard solutions of aflatoxins B1, B2, G1 and G2, fumonisin B1 and B2, A and B trichothecenes (nivalenol, deoxynivalenol, 3-acetyl-deoxynivalenol, fusarenone X, diacetoxyscirpenol, T-2 toxin, HT-2 toxin), zearalenone, ochratoxin A, and patulin were obtained from Romer Labs (Tulln, Austria). All the solvents applied for both the extraction and analysis steps, methanol, acetonitrile formic acid and acetic acid, were HPLC-grade and were purchased from Sigma-Aldrich (Milan, Italy), while Bi-distilled water was produced in-house by using a Milli-Q System (Millipore, Bedford, MA, USA). Salts used for extraction as for the preparation of the eluents as sodium chloride and ammonium acetate were obtained from Sigma-Aldrich (Milan, Italy).
4
Quantifying Mycotoxins in Food Samples
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HPLC grade acetonitrile, methanol, water and formic acid were purchased from Fisher Scientific. Japanese aflatoxin mixture (25 μg/mL) from Sigma-Aldrich; deoxynivalenol (25 µg/mL), nivalenol (25 µg/mL), zearalenone (10 µg/mL), ochratoxin A (10 µg/mL) from Romer Labs; diacetoxyscirpenol (100 µg/mL), T-2 toxin (100 µg/mL), HT-2 toxin (100 µg/mL), fumonisin B1 (100 µg/mL), fumonisin B2 (100 µg/mL), and fumonisin B3 (100 µg/mL) from Trilogy. QuEChERS EN extraction salts and Captiva EMR-Lipid cartridge were acquired from Agilent (CA, USA).
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