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20 protocols using texture exponent software

1

Cupcake Firmness Measurement Protocol

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The firmness of the freshly prepared cupcakes was measured by using a TA-XT plus Texture Analyzer with the Texture Exponent software version 2.0.7.0 (Stable Micro-systems, Godalming, UK). The test speed was 1 mm/s, and the press distance was 5 mm. A trigger force of 5× g was enforced on the entire standing cupcake by a round probe with a diameter of 36 mm (P/36).
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2

Evaluating CB:OG Systems Consistency

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The evaluation of the consistency of the CB:OG systems was carried out using a TA.HDPlus Texture Analyzer (Stable Micro Systems, Ltd., Godalming, UK) provided with Texture Exponent software (version 6.1.16.0; Stable Micro Systems, Ltd., Godalming, UK) and equipped with a 30 kg load cell. A penetration test was performed using a polyoxymethylene cylindrical probe (P/10, 10 mm Ø) that penetrated the sample to a depth of 6 mm at a rate of 1 mm/s with a 0.1 N trigger force. After 24 h of storage at 4 °C, the samples were tempered and measured directly in the plastic buckets at both 10 and 20 °C. The maximum force and the area under the force-distance curve at the defined penetration distance were recorded and used as indicators for the consistency/hardness of the CB:OG systems. Three replicates were performed with samples prepared on different days (two batches).
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3

Texture Analysis of Protein-Based Foods

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The texture of protein-based foods was measured with a TA.HD Plus Texture Analyser (Stable Micro Systems Ltd., U.K.) equipped with a 50-kg load cell. All textural analyses were carried out using Texture Exponent software version 6.1.5.0. (Stable Micro Systems Ltd.) by a slight modification of a previously described method (Sakamoto et al., 2006 (link)). The preparation procedure (duration of cooking; time period between cooking and testing) was the same for all tested samples. The hardness of the steamed sample was measured for each enzyme using an 10-mm cylinder probe with the following instrument settings: measured force in compression, pre-test speed: 1.0 mm/s; test speed: 2.0 mm/s; post-test speed: 10.0 mm/s; strain: 70%; trigger force: 10 g. The maximum force correlates to the hardness of the sample. The tests were performed on 10 replicates per sample. The sample was positioned centrally under the probe during testing.
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4

Texture Analysis of Extruded Samples

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The extrusion properties of the samples were determined by using a TA-XT plus Texture Analyzer equipped with the Texture Exponent software (Stable Microsystems, Godalming, UK). A back extrusion assay was carried out using a bucket of 5 cm in diameter and 7.5 cm in height and a compression probe of 4.9 cm in diameter. The distance force was 15mm, the compression rate 1mm/s, and the trigger force 10g. From the force time profiles obtained, the area under the curve and the maximum force achieved were recorded.
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5

Texture Analysis of Crackers

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Hardness, fracturability, and total work of the crackers were measured using the Texture Analyzer (TA.XT. plus C, Stable Micro Systems, Surrey, UK) equipped with the Warner Bratzler blade (HDP/BS). Data were evaluated using the Texture Exponent Software (Version 6.1.18.0, Stable Micro Systems). The cracker was placed on the slotted blade insert and the blade moved downwards at a speed of 1 mm/sec and at a force load of 5 mm/sec, until the cracker fracture was achieved. Hardness was calculated as the maximum force required to break the sample. Fracturability (the distance at the point of break) and total energy (total area work) were also determined.
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6

Dough Texture Analysis via Sphere Penetration

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The texture of the dough pieces was analysed using a TA-XT2 texture analyser equipped with Texture Exponent software (Stable Micro systems Ltd., England). A sphere penetration test was performed following the method described by Rodriguez-Garcia et al.51 (link) with some modifications. A stainless-steel spherical probe (P/0.75) was used to compress the centre of the dough piece at 60% strain deformation, with a trigger force of 0.049 N, and test speed 1 mm/s. The maximum force (N), denoted as the peak of the curve was measured. The test was performed in ten dough pieces of each dough batch.
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7

Biscuit Texture Analysis Using Texture Analyzer

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The texture of the biscuits was analysed using a TA-XT2 texture analyser equipped with the Texture Exponent software (Stable Micro systems Ltd., England). Biscuits were broken using the three-point-bending rig probe (A/3PB) using the following conditions: supports 15 mm apart, the probe moved 5 mm with a test speed of 1 mm/s, and a trigger force of 0.05 N. The parameters measured were the force at break (N), and the distance at break (mm) as the fracture strength and the fracturability, respectively. The analysis was performed on ten biscuits per formulation batch.
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8

Texture and Spreadability Analysis of Samples

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TA-XT plus Texture Analyser equipped with the Texture Exponent software (Stable Microsystems, Godalming, UK) was used to determinate the texture properties of the samples.
Spreadability of samples was measured using a TTC spreadability rig (HDP/SR) (Stable Microsystems, Godalming, UK) attachment. Samples were filled into a female cone (90°) with special attention to avoid bubbles formation. Samples were penetrated to 22 mm using a corresponding male cone (90°) attachment at a speed of 1 mm/s. Force expressed in Newtons and area under the curve (AUC; N·s), as a measure of spreadability, were recorded. Measurements were performed in triplicate.
A back extrusion test was conducted using a bucket of 50 mm diameter and 75 mm height with a compression probe of 49 mm diameter. The samples were extruded by 15 mm, at a speed of 1 mm/s. From the force time profiles, the AUC (N·s), a measure of consistency, was recorded. Measurements were performed in triplicate.
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9

Texture Analysis of Dysphagia Thickeners

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The texture measurements were conducted with a TA-XTPlus Texture Analyzer (Stable Microsystems, Goldalming, UK) equipped with Texture Exponent software (version 2.0.7.0. Stable Microsystems), with a 30-kg load cell and 0.1 N of trigger force. The texture analyzer captures the force, distance, and time needed to—depending on the probe—attach, extrude, penetrate, or compress the dysphagia thickener whilst the arm of the texture analyzer moves down and returns to its initial position. Data are registered once the trigger force is achieved. These data are also shown graphically whilst the test is performed.
Four tests frequently used in the evaluation of textural attributes of semi-solid food products were performed: two back-extrusion tests and two penetration tests [27 (link)]. The tests are extensively described in Section 2.3.1 and Section 2.3.2.
All tests were conducted at 1, 3, 5, and 10 mm/s and at 25 °C. This was the room temperature, as no device to maintain the temperature in the texture analyzer was available. The selection of the temperature is important as the physical properties depend on it; for example, viscosity, according to the Arrhenius equation, depends on temperature. For the four tests, the pretest and the post-test speeds were 10 mm/s. Table 3 summarizes the tests and parameters. Three replicates were performed per sample.
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10

Texture and Spreadability Analysis of Samples

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A TA-XT plus Texture Analyzer equipped with the Texture Exponent software (Stable Microsystems, Godalming, UK) was used to determinate the texture properties of the samples.
Spreadability of samples was measured using a TTC Spreadability Rig (HDP/SR) attachment. Samples were filled into a female cone (90 • angle), with special attention made to avoid bubbles formation, and were penetrated 22.5 mm using the corresponding male cone (90 • angle) at a speed of 1 mm/s. Force expressed in N, as a measurement of firmness, and area under the curve (AUC; N s), as a measurement of spreadability, were recorded. Experiments were performed in duplicate.
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