Human lung cancer cell lines (A549, NCI-H1975, Calu-3) and human normal epithelial cells BEAS-2B were purchased from Procell (https://www.procell.com.cn ). The A549 cells were cultured in Ham’s F-12K Medium (Thermo Fisher, Waltham, MA, USA). The NCI-H1975 cells were cultured in RPMI1640 medium (Hyclone, Logan, UT, USA). The Calu-3 cells were cultured in modified Eagle’s medium (MEM, containing NEAA, Procell, Wuhan, China). The BEAS-2B cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone, USA). All cells were cultured in the specific mediums supplemented with 10% fetal bovine serum (Hyclone, USA), penicillin sodium (100 U/mL) and streptomycin (100 mg/mL). The cells were incubated in the thermostatic cell incubator at 37 °C with 5% CO2. Total RNA was extracted using the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). Quantitative PCR (qPCR) was performed using the ABI 7900 qPCR system. Relative RNA expression was normalized to GAPDH and calculated by the 2−ΔΔCt method. The primers were listed in Table 1 .
Calu 3
Manufactured by Procell
Sourced in China
The Calu-3 is a cell line derived from a human lung adenocarcinoma. It is commonly used in research applications to study various cellular processes and functions.
Automatically generated - may contain errors
Lab products found in correlation
Beas 2b, by Procell (3 mentions)
Calu 3, by Thermo Fisher Scientific (3 mentions)
Ham s f 12k medium, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hcc827, by Procell (2 mentions)
Rneasy plus mini kit, by Qiagen (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin g, by Thermo Fisher Scientific (1 mentions)
Beas 2b, by Thermo Fisher Scientific (1 mentions)
Nci h1975, by Thermo Fisher Scientific (1 mentions)
Sk mes 1, by Thermo Fisher Scientific (1 mentions)
Nci h1299, by Thermo Fisher Scientific (1 mentions)
Nci h226, by Thermo Fisher Scientific (1 mentions)
Nci h520, by Thermo Fisher Scientific (1 mentions)
Beas 2b, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Bronchial epithelial cell growth medium bullet kit begm, by Lonza (1 mentions)
Nci h520, by Procell (1 mentions)
Beas 2b, by Lonza (1 mentions)
Nci h1975, by Procell (1 mentions)
8 protocols using calu 3
1
Comparative Analysis of Lung Cancer Cell Lines
2
Air-Liquid Interface Culture of Bronchial Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Air-liquid interface cultured differentiated human bronchial epithelial cell were performed (see Supplementary Material
P. aeruginosa PAO1 strain (ATCC) was resuscitated and incubated overnight in a shaker, and the logarithmic growth phase was diluted to different colony forming unit (CFU)s with LB broth and added to the upper chamber of the transwell.
P. aeruginosa PAO1 strain (ATCC) was resuscitated and incubated overnight in a shaker, and the logarithmic growth phase was diluted to different colony forming unit (CFU)s with LB broth and added to the upper chamber of the transwell.
3
Characterization of NSCLC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non-cancerous, immortalized human lung bronchial epithelial cell line BEAS-2B was purchased from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China). Normal fetal lung fibroblast cell HFL1; human LUAD cell lines Calu-3, NCI-H1975, and NCI-H1395; LUSC line NCI-H520; and other NSCLC cell lines A549 and NCI-H1299 were purchased from Procell (Wuhan, China). LUSC lines NCI-H226 and SK-MES-1 were purchased from Shanghai Chinese Academy of Science Cell Bank (Shanghai, China). BEAS-2B was grown in Bronchial Epithelial Cell Growth Medium BulletKit (BEGM; Lonza, Basel, Switzerland). HFL1 was cultured in Ham’s F-12K medium (Gibco, Pittsburgh, PA, USA). Calu-3 and SK-MES-1 were cultured in Eagle’s minimum essential medium (MEM; Gibco). NCI-H1975, NCI-H1395, NCI-H1299, NCI-H520, and NCI-H226 were maintained in RPMI-1640 (Gibco). All cell lines, except for BEAS-2B, were supplemented with 10% fetal bovine serum (FBS), streptomycin (100 μg/mL), and penicillin G (100 U/mL; Gibco). All cell lines were authenticated by short tandem repeat (STR) profiling. Cells were incubated at 37°C in a humidified atmosphere with 5% CO2.
4
Cell Culture Conditions for Viral Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Huh-7, HPA-3, Caco-2, and Vero E6 cells were introduced from Kunming Cell Bank and Calu-3 was purchased from Procell Life Science＆Technology Co., Ltd. Cells were grown in Dulbecco's Modified Eagle medium (DMEM) high glucose (Gibco, Beijing) supplemented with 10% fetal bovine serum (FBS) (Gibco, New Zealand), 1% penicillin/streptomycin (Solarbio, Beijing), and kept in a humidified 5% CO2 incubator at 37 °C.
5
Cell Line Cultivation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human embryonic kidney (HEK) 293T, human immortalized bronchial epithelial BEAS-2B, and NSCLC H1299, A549, LTEP-α-2, H226, Calu-3, HCC827, and H1975 cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). The cells were cultured in DMEM high-glucose medium or RPMI-1640 medium (HyClone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) and 1% penicillin-streptomycin (Beyotime, Shanghai, China) in a humidified atmosphere with 5.0% CO2 at 37 °C. The cell supplier had confirmed the genetic characteristics of these cells. All these cell lines were passaged within 6 months.
6
Cultivation of NSCLC and Bronchial Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4 human NSCLC cell lines (A549, H1299, HCC827, and Calu-3) and 1 human bronchial epithelial cell line (BEAS-2B) were ordered from Procell (China) and cultured appropriately. In detail, A549 was grown in Ham's F-12K (Procell, China). H1299 and HCC827 were cultivated in RPMI-1640 (Procell, China), whereas Calu-3 was inoculated in MEM (Gibco, USA). All the media mentioned above were supplemented with 10% FBS (Procell, China) and 1% p/s (Procell, China). BEAS-2B was maintained in BEGM (Procell, China). All of them were maintained in an incubator (MG80, Kuansons, China) containing 5% CO2 at 37°C.
7
Characterization of APOE Isoforms and ACE2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
293T cells (Cat#CRL-3216) were purchased from ATCC, BEAS-2B(Cat#C6106) cells from Beyotime, 293T-ACE2 (Cat#HEK-293T-LV-0582) cells were purchased from BrainVTA, and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). Calu-3 (Cat#CL-0054) cells were purchased from Procell, and cultured in Modified Eagle Medium (MEM) supplemented with 10% FBS and 1% P/S. Murine astrocytes overexpressing hAPOE3 or hAPOE4 were a kind gift from David Holtzman (Washington University), and maintained in DMEM with 20% FBS and 1% P/S. All cells were tested negative for Mycoplasma spp. Various constructs encoding Flag- or HA- tagged APOE, HA- and His-tagged full-length or fragmented ACE2, were transfected into 293T or 293T-ACE2 cells with polyethyleneimine (PEI). Cells were subjected to various analyses 24 h after the transfection.
8
Cell Line Characterization and Maintenance
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human lung cancer cell lines A549, NCI-H1437, and NCI-H1299, human embryonic kidney cell line HEK293T, human hepatoma carcinoma cell line HepG2 and human colon cancer cell line SW620 were obtained from Cell Bank, Type Culture Collection, Chinese Academy of Sciences, Shanghai, P.R. China. Human lung cancer cell line Calu-3 was obtained from Procell Life Science & Technology, Wuhan, Hubei, P.R. China. HEK293T cells were maintained in DMEM (Gibco). HepG2 and Calu-3 cells were maintained in Minimum Essential Media (Gibco). SW620 cells were maintained in Leibovitz's L-15 medium (Gibco). A549, NCI-H1437, and NCI-H1299 cells were maintained in RPMI1640 medium (Gibco). All media were supplemented with 10% FBS (HyClone), 100 UÁmL À1 penicillin, and 100 mgÁmL À1 streptomycin (Life Technologies). Cells were cultured at 37 C in humidified incubator containing 5% CO 2 . All cell lines were identified by STR profiling by the source. Cells were expanded after being received and subsequently stored in liquid nitrogen. The storage vials were thawed for experiments and used in less than 2 months. All cell lines were confirmed negative for Mycoplasma.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!