A volume of 4 ml of peripheral venous blood was drawn from the antecubital fossa by venipuncture method, using a 20-gauge needle with a 5-ml syringe. The blood was then transferred to the vacutainer tubes and immediately transferred to the laboratory. The blood sample was allowed to clot at room temperature and after 1 h, serum was separated from the blood by centrifuging at 3,000 rpm for 15 min. 0.5 ml of serum was utilized for PPBS by glucose oxidase-peroxidase method (Kit-M/S Excel Diagnostics Pvt. Ltd., India) and HbA1C estimation (Kit-Nycocard, India) was performed in an automatic analyzer (BIO-RAD D-10-HPLC ANALYZER) only for Group-II individuals. The serum of all individuals from both groups was stored in Eppendorf tubes for adiponectin and high-sensitivity-C-reactive protein (hs-CRP) estimation.
D 10 hplc analyzer
Manufactured by Bio-Rad
Sourced in India, United States
The D-10-HPLC ANALYZER is a high-performance liquid chromatography (HPLC) system designed for laboratory applications. It is capable of analyzing and quantifying various compounds in liquid samples. The core function of the D-10-HPLC ANALYZER is to separate, identify, and measure the concentrations of different components within a sample.
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Lab products found in correlation
3 protocols using d 10 hplc analyzer
1
Venous Blood Collection and Serum Analysis
2
Comprehensive Cardiometabolic Biomarker Profiling
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Lipid profile (TC, HDL, LDL, TG), uric acid, homocysteine and hsCRP were estimated by Roche Cobas C501 autoanalyzer (Manheim Germany) using commercially available kits from Roche by photometric method. Low-density lipoprotein (LDL) cholesterol levels were calculated using the Friedewald equation. Fibrinogen was estimated by ACL ELITE PRO coagulation analyzer (MIAMI, USA) using commercially available reagent from IL (MIAMI, USA) by photoconductance method. HbA1c was estimated by BIO-RAD D-10 HPLC analyzer (USA) using commercially available kit from BIO-RAD (USA) by HPLC. Troponin T testing was done qualitatively using commercially available kit from Roche.
3
Measurement of HbA1c for Glycemic Control
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HbA1c, an indicator of glycemic control, was the dependent variable in our analyses. Participants were asked to come into the designated diagnostic center after an overnight fast for ≥8 hours. Fasting venous blood was collected between 08:00 and 11:00 for measurement of HbA1c. A 3-ml venous blood sample was drawn from all participants into tubes containing sodium fluoride, and plasma was separated into new tubes and analyzed within 1 hour of collection. HbA1c was measured by high-performance liquid chromatography (D-10 HPLC Analyzer; Bio-Rad, Hercules, CA). The same laboratories in each center involved in the measurement of HbA1c were committed to participating in external quality affirmation schemes, assuring that laboratories with equivalent equipment could produce results that were comparable to each other. In accordance with the American Diabetes Association (ADA) guidelines, HbA1c < 7% was taken to indicate good glycemic control, while HbA1c ≥ 7% indicated poor glycemic control36 .
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