Anti id1 c 20

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti Id1 (C-20) is a primary antibody product offered by Santa Cruz Biotechnology. It is designed to detect the Id1 protein, which plays a role in cellular processes. The antibody can be used for various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of Id1 in different biological systems.

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Lab products found in correlation

Anti lamin b c 20, by Santa Cruz Biotechnology (1 mentions) Anti id3 c 20, by Santa Cruz Biotechnology (1 mentions) Anti e2a n 649, by Santa Cruz Biotechnology (1 mentions) Anti β actin ac 15, by Merck Group (1 mentions) Complete mini, by Roche (1 mentions) Anti sox2, by Cell Signaling Technology (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti igf 1r, by Cell Signaling Technology (1 mentions) Anti β actin clone c4, by Merck Group (1 mentions)

2 protocols using anti id1 c 20

Immunoblotting and Co-immunoprecipitation Protocol

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Cells were lysed in NP-40 buffer in the presence of protease inhibitor cocktail (Complete Mini, Roche Diagnostics). The following antibodies were used for immunoblotting and co-immuno-precipitation: Anti PU.1 (H-135, Santa Cruz), anti Id1 (C-20, Santa Cruz), anti Id2 (C-20, Santa Cruz), anti Id3 (C-20, Santa Cruz), anti E2A (N-649, Santa Cruz), anti Lamin B (C-20, Santa Cruz), anti Gfi1 (R&D systems) and anti β-Actin (Ac-15, Sigma-aldrich).

Fraszczak J., Helness A., Chen R., Vadnais C., Robert F., Khandanpour C, & Möröy T. (2016). Threshold Levels of Gfi1 Maintain E2A Activity for B Cell Commitment via Repression of Id1. PLoS ONE, 11(7), e0160344.

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Immunoblotting of Stem Cell Markers

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The following antibodies were used: anti-ID1 (C-20, 1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-IGF2 (clone S1F2, 1:5000; Millipore), anti-IGF1R (no. 9750; Cell Signaling Technology), anti-p-Akt (no. 4060; Cell Signaling Technology), anti-Sox2 (no. 3579; Cell Signaling Technology) and anti-β-actin (clone C4, 1:20 000; Millipore) antibodies.

Tominaga K., Shimamura T., Kimura N., Murayama T., Matsubara D., Kanauchi H., Niida A., Shimizu S., Nishioka K., Tsuji E.I., Yano M., Sugano S., Shimono Y., Ishii H., Saya H., Mori M., Akashi K., Tada K.I., Ogawa T., Tojo A., Miyano S, & Gotoh N. (2016). Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells. Oncogene, 36(9), 1276-1286.

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