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Cov2 2130

Manufactured by Regeneron Pharmaceuticals
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COV2-2130 is a laboratory equipment product designed for research purposes. It serves as a tool for researchers to study and analyze various aspects related to the COVID-19 virus.

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Lab products found in correlation

Regn10933, by Regeneron Pharmaceuticals (3 mentions) Regn10987, by Regeneron Pharmaceuticals (3 mentions) Cov2 2196, by Regeneron Pharmaceuticals (3 mentions) Rprotein a sepharose, by GE Healthcare (2 mentions) Expi293 cell, by Thermo Fisher Scientific (2 mentions)

3 protocols using cov2 2130

1

Monoclonal Antibody Production and Testing

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The monoclonal antibodies tested in this study were constructed and produced at Columbia University, except for REGN10933, REGN10987, COV2-2196, and COV2-2130, which were provided by Regeneron Pharmaceuticals, Inc., and CB6, which was provided by Baoshan Zhang and Peter D. Kwong (10 (link)).
Mathema B., Chen L., Wang P., Cunningham M.H., Mediavilla J.R., Chow K.F., Luo Y., Zhao Y., Composto K., Zuckerman J., Zody M.C., Wilson N., Lee A., Oschwald D.M., Liu L., Iketani S., Germer S., Fennessey S., Wang M., Kramer Y., Toole P., Maniatis T., Ho D.D., Perlin D.S, & Kreiswirth B.N. (2022). Genomic Epidemiology and Serology Associated with a SARS-CoV-2 R.1 Variant Outbreak in New Jersey. mBio, 13(5), e02141-22.
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2

Antibody Expression and Purification

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Antibodies were expressed as previously described18 (link). Briefly, Vh and Vl genes for each antibody were codon optimized and synthesized (GenScript), and then inserted into mammalian expression vectors. These plasmids were transiently transfected into Expi293 cells (Thermo Fisher) using polyethylenimine and cultured for 5 days, and then the antibody was purified by affinity chromatography using rProtein A Sepharose (GE). REGN10933, REGN10987, COV2-2130 and COV2-2196 were provided by Regeneron Pharmaceuticals, Brii-196 and Brii-198 were provided by Brii Biosciences, and CB6 was provided by B. Zhang and P. Kwong (NIAID).
Iketani S., Liu L., Guo Y., Liu L., Chan J.F., Huang Y., Wang M., Luo Y., Yu J., Chu H., Chik K.K., Yuen T.T., Yin M.T., Sobieszczyk M.E., Huang Y., Yuen K.Y., Wang H.H., Sheng Z, & Ho D.D. (2022). Antibody evasion properties of SARS-CoV-2 Omicron sublineages. Nature, 604(7906), 553-556.
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3

Antibody Expression and Purification Protocol

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Antibodies were expressed as previously described17 (link). Heavy chain variable and light chain variable genes for each antibody were synthesized (GenScript), then transfected into Expi293 cells (Thermo Fisher Scientific) and purified from the supernatant by affinity purification using rProtein A Sepharose (GE). REGN10987, REGN10933, COV2-2196 and COV2-2130 were provided by Regeneron Pharmaceuticals; Brii-196 and Brii-198 were provided by Brii Biosciences; CB6 was provided by B. Zhang and P. Kwong (National Institutes of Health (NIH)) and ZCB11 was provided by Z. Chen (University of Hong Kong).
Wang Q., Guo Y., Iketani S., Nair M.S., Li Z., Mohri H., Wang M., Yu J., Bowen A.D., Chang J.Y., Shah J.G., Nguyen N., Chen Z., Meyers K., Yin M.T., Sobieszczyk M.E., Sheng Z., Huang Y., Liu L, & Ho D.D. (2022). Antibody evasion by SARS-CoV-2 Omicron subvariants BA.2.12.1, BA.4 and BA.5. Nature, 608(7923), 603-608.
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