Lipofectamine, pcDNA3 plasmid, Geneticin, cell culture medium, and other culture reagents were from Invitrogen-Thermo Fischer Scientific (MA, US). Ultrafiltered fetal bovine serum (FBS) was from Natocor (Cordoba, Argentina). Restriction enzymes and other molecular biology reagents were from Promega (WI, US). [1-14C]oleic acid ([14C]-OA) and [6-3H]thymidine were from Amersham Biosciences-GE (MA, US). Fatty acid-free bovine serum albumin (BSA), mouse anti-β-actin monoclonal antibody, anti-mouse IgG peroxidase conjugate and anti-rabbit IgG peroxidase conjugate were purchased from Merck-Sigma (Darmstadt, Germany). Silica gel 60 chromatography plates and analytical-grade solvents were from Merck (Darmstadt, Germany).
Silica gel 60 chromatography plates
Manufactured by Merck Group
Sourced in Germany, Spain
Silica gel 60 chromatography plates are a type of thin-layer chromatography (TLC) media used for the separation and analysis of a variety of compounds. They consist of a thin layer of silica gel applied to a rigid support, typically glass or aluminum. The silica gel provides a stationary phase for the separation process, while the mobile phase, such as a solvent, carries the sample components through the media. These plates are commonly used in analytical chemistry and organic synthesis for the purification and identification of organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Analytical grade solvents, by Merck Group (1 mentions)
Restriction enzymes, by Promega (1 mentions)
Cell culture medium, by Thermo Fisher Scientific (1 mentions)
Anti mouse igg peroxidase conjugate, by Merck Group (1 mentions)
Pcdna3 plasmid, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti β actin antibody, by Merck Group (1 mentions)
Fatty acid free bovine serum albumin, by Merck Group (1 mentions)
Anti rabbit igg peroxidase conjugate, by Merck Group (1 mentions)
Lipofectamine, by Thermo Fisher Scientific (1 mentions)
Geneticin, by Thermo Fisher Scientific (1 mentions)
2 protocols using silica gel 60 chromatography plates
1
Cell Culture and Molecular Techniques
2
Aflatoxin B1 detection protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After AFB1 extraction with chloroform, samples were filtered using 0.45 µm syringe filters (Fisherbrand, Spain) and an aliquot of 1 mL was evaporated in a vacuum concentrator, Eppendorf™ Concentrator Plus with Pump and GB Plug (Fisher Scientific, Madrid, Spain).
Silica gel 60 chromatography plates (Merck, Germany) were used, and AFB1 presence was determined according to the protocols described elsewhere [37 (link),38 (link)].
Samples and AF standards were re-suspended with 500 µL toluene/acetonitrile (95:5) (Panreac, Spain). Then, 10 µL of each sample was spotted on the plate. Toluene/acetone/acetonitrile (1:1:1 (LabKem, Barcelona, Spain)) was used as a mobile phase. Toxins were visualized under ultraviolet light (Spectronics, Westbury, NY, USA).
Silica gel 60 chromatography plates (Merck, Germany) were used, and AFB1 presence was determined according to the protocols described elsewhere [37 (link),38 (link)].
Samples and AF standards were re-suspended with 500 µL toluene/acetonitrile (95:5) (Panreac, Spain). Then, 10 µL of each sample was spotted on the plate. Toluene/acetone/acetonitrile (1:1:1 (LabKem, Barcelona, Spain)) was used as a mobile phase. Toxins were visualized under ultraviolet light (Spectronics, Westbury, NY, USA).
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