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KYSE30 is a cell line derived from human esophageal squamous cell carcinoma. It is maintained in the Japanese Collection of Research Bioresources Cell Bank and available for purchase by researchers.

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Lab products found in correlation

Kyse510, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Kyse140, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Kyse450, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Kyse150, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse1440, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse70, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse410, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse270, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse180, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Rpmi 1640, by Merck Group (2 mentions) Kyse170, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Rmpi 1640, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Fujifilm (1 mentions) Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions) Het 1a, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)

8 protocols using kyse30

1

ESCC Cell Line Culturing Protocol

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The human ESCC cell lines KYSE-30, KYSE-150 and KYSE-510 were from the JCRB cell bank (Japanese Collection of Research Bioresources cell bank) and cultured in RMPI 1640 (GIBCO) supplemented with 10% fetal bovine serum (FBS, GIBCO) and 1% penicillin/streptomycin (GIBCO) at 37 °C with 5% CO2. Vero cells (ATCC-CCL-81) were cultured in DMEM (GIBCO) supplemented with 10% fetal bovine serum (FBS, GIBCO) and 1% penicillin/streptomycin (GIBCO) at 37 °C with 5% CO2.
Wu A., Li Z., Wang Y., Chen Y., Peng J., Zhu M., Li Y., Song H., Zhou D., Zhang C., Lv Y, & Zhao Z. (2023). Recombinant measles virus vaccine rMV-Hu191 exerts an oncolytic effect on esophageal squamous cell carcinoma via caspase-3/GSDME-mediated pyroptosis. Cell Death Discovery, 9, 171.
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2

Human ESCC Cell Culture Protocol

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Human ESCC cell lines (KYSE30, KYSE140, KYSE520, and KYSE1440) were purchased from the Japanese Collection of Research Bioresources (JCRB, Tokyo, Japan). These cell lines are authenticated using STR profiling in the JCRB. Cells were cultured in Dulbecco's Modified Eagle (DMEM) medium (Nacalai tesque, Kyoto, Japan), supplemented with 1% penicillin-streptomycin and 10% heat-inactivated fetal calf serum (FCS). The culture was grown in cell culture dishes in a humidified atmosphere containing 5% CO2 at 37°C. Cells were washed with phosphate-buffered saline without calcium and magnesium (PBS, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and harvested with Trypsin-EDTA (0.25%) (ThermoFisher, Massachusetts, USA). The harvested cells were processed immediately for imaging as described below.
Akashi T., Okumura T., Terabayashi K., Yoshino Y., Tanaka H., Yamazaki T., Numata Y., Fukuda T., Manabe T., Baba H., Miwa T., Watanabe T., Hirano K., Igarashi T., Sekine S., Hashimoto I., Shibuya K., Hojo S., Yoshioka I., Matsui K., Yamada A., Sasaki T, & Fujii T. (2023). The use of an artificial intelligence algorithm for circulating tumor cell detection in patients with esophageal cancer. Oncology Letters, 26(1), 320.
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3

Culturing ESCC Cell Lines

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ESCC cell lines of human (KYSE-30 and TE series) were acquired from the Japanese Collection of Research Bioresources Cell Bank, the Cell Resource Center for Biomedical Research, and the Riken BioResource Center Cell Bank. These cell lines were cultured in RPMI 1640 or DMEM added with 10% FBS in a 5% CO2 atmosphere at 37°C.
Nakamura K., Baba Y., Kosumi K., Harada K., Shigaki H., Miyake K., Kiyozumi Y., Ohuchi M., Kurashige J., Ishimoto T., Iwatsuki M., Sakamoto Y., Yoshida N., Watanabe M., Nakao M, & Baba H. (2016). UHRF1 regulates global DNA hypomethylation and is associated with poor prognosis in esophageal squamous cell carcinoma. Oncotarget, 7(36), 57821-57831.
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4

Culturing Esophageal Cell Lines

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Human ESCC cell lines including, KYSE30 (#0188) and KYSE450 (#1430) were from Japanese Collection of Research Bioresources (JCRB; NIBIO, Ibaraki, Osaka, Japan), and the normal esophagus epithelial cell line HET-1A (#2692) was from American Type Culture Collection (ATCC; Manassas, VA, USA). The KYSE30 cells were cultured in DMEM (Hyclone, Logan, UT, USA) with 2% fetal bovine serum (FBS; Hyclone), and KYSE30 cells were cultured in Ham’s F12 and RPMI1640 medium (1 to 1 mix; Hyclone) with 2% FBS, HET-1A cells were in DMEM plus 10% FBS. All the cells were incubated in a humidified atmosphere of 5% CO2 at 37 °C.
Zhou S., Guo Z., Zhou C., Zhang Y, & Wang S. (2021). circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway. Cancer Cell International, 21, 250.
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5

Culturing and Characterizing Esophageal Squamous Cell Carcinoma Lines

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A total of 21 ESCC cell lines, specifically KYSE30, KYSE70, KYSE140, KYSE150, KYSE180, KYSE270, KYSE410, KYSE450, KYSE510, KYSE590, KYSE890, KYSE1170, KYSE1260, KYSE1440, NUEC2, TE1, TE2, TE3, WSSC, TT, and TTn, along with Het‐1A, a non‐cancerous epithelial cell line, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), the American Type Culture Collection (Manassas, VA, USA), or were established at Nagoya University.
5 (link) The cells were cultured in a humidified incubator at 37°C with an atmosphere composition of 5% CO2 using RPMI‐1640 (Sigma‐Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum and antibiotics.
24 (link)
Shinozuka T., Kanda M., Sato Y., Shimizu D., Tanaka C., Umeda S., Inokawa Y., Hattori N., Hayashi M., Nakayama G, & Kodera Y. (2023). Increased STX3 transcript and protein levels were associated with poor prognosis in two independent cohorts of esophageal squamous cell carcinoma patients. Cancer Medicine, 12(24), 22185-22195.
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6

Cell Line Culturing Protocol

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ESCC cell lines, KYSE30 and KYSE110, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). SW480 and HCT116, Colon cancer cell lines, were purchased from the American Type Culture Collection (Manassas, VA, USA) and RIKEN Bio Resource Center (Ibaraki, Japan), respectively. All cell lines were cultured in RPMI‐1640 (Sigma‐Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (Biological Industries Kibbutz Beit Haemek and, Haifa, Israel) and 1% antibiotic‐antimycotic (Thermo Fisher Scientific, Waltham, MA, USA).
Thar Min A.K., Okayama H., Saito M., Ashizawa M., Aoto K., Nakajima T., Saito K., Hayase S., Sakamoto W., Tada T., Hanayama H., Saze Z., Momma T., Ohki S., Sato Y., Motoyama S., Mimura K, & Kono K. (2018). Epithelial‐mesenchymal transition‐converted tumor cells can induce T‐cell apoptosis through upregulation of programmed death ligand 1 expression in esophageal squamous cell carcinoma. Cancer Medicine, 7(7), 3321-3330.
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7

Culturing Esophageal and Biliary Cancer Cells

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Esophageal and biliary cancer were selected as the primary superficial cancers in our investigation. The human esophageal cancer cell lines KYSE30, KYSE70, and KYSE170 cells were obtained from the JCRB cell bank (Osaka, Japan). The KYSE30 cells were cultured in Dulbecco’s modified Eagle medium (D-MEM) supplemented with 2% fetal bovine serum (FBS). The KYSE70 cells were cultured in D-MEM supplemented with 5% FBS. The KYSE170 cells were cultured in the RPMI-1640/nutrient mixture F-12 medium supplemented with 2% FBS. The human cholangiocarcinoma cell lines HuCCT-1 and KKU-213 cells were obtained from the JCRB cell bank. The HuCCT-1 cells were cultured in RPMI-1640 medium supplemented with 10% FBS. The KKU-213 cells were cultured in the D-MEM supplemented with 10% FBS.
All cell culture media were supplemented with 2 mM L-glutamine solution without antibiotics. The cells were cultured in a humidified incubator with 5% CO2 at 37 °C.
Nakada Y., Sugihara T., Tanaka M., Hamamoto W., Kanda T., Sakaguchi T., Kurumi H., Onoyama T., Ikebuchi Y., Takata T., Isomoto H, & Yamaguchi N. (2024). The Potential of Narrow-Band Imaging as a Novel Light Source for Photodynamic Therapy for Superficial Cancers via Endoscopes. Gastroenterology Research, 17(2), 72-81.
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8

Esophageal and Neuroblastoma Cell Lines Cultivation

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Nine human ESCC cell lines, KYSE30, KYSE140, KYSE170, KYSE180, KYSE220, KYSE270, KYSE410, KYSE450, and KYSE510, were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank [22 (link)]. Two neuroblastoma cell lines, IMR-32 and KELLY, were obtained from the JCRB Cell Bank and Public Health England, respectively. KYSE140 was cultured in Ham's F12 medium containing 2% (v/v) FBS; KYSE30, KYSE170, KYSE180, KYSE220, KYSE270, KYSE410, KYSE450, and KYSE510 were cultured in Ham's F12/RPMI1640 medium containing 2% (v/v) FBS; IMR-32 was cultured in MEM medium containing 10% (v/v) FBS and non-essential amino acid (NEAA); and KELLY was cultured in RPMI1640 medium containing 10% (v/v) FBS.
Nakazato H., Takeshima H., Kishino T., Kubo E., Hattori N., Nakajima T., Yamashita S., Igaki H., Tachimori Y., Kuniyoshi Y, & Ushijima T. (2016). Early-Stage Induction of SWI/SNF Mutations during Esophageal Squamous Cell Carcinogenesis. PLoS ONE, 11(1), e0147372.
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