For silver nanoparticle synthesis, silver nitrate (≥99%), sodium citrate dihydrate (≥99%), potassium iodide (≥99%), phosphate buffer saline solution (PBS), sodium chloride (≥98%), anhydrous ethanol, and dithiothreitol (1 M) were purchased from Sigma-Aldrich, and ascorbic acid (≥99%) was purchased from Sigma Life Science. Custom modified miRNA-148b was received from Trilink Bio Technologies. Diethylpyrocarbonate water was purchased from Ambion, sodium dodecyl sulfate (≥99%) from VWR, and tris(hydroxymethyl) aminomethane (≥99.9%) solid from Amresco. The 1% polystyrene nanoparticle solution with amine functionalization was purchased from Nanocs, and succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (≥99%) was received from Thermo Fisher. Nanopure water with resistivity greater than 18.2 MΩ was used as a solvent during the synthesis of the miRNA-functionalized nanoparticles, as well as for spectroscopic measurements.
Phosphate buffer saline solution
Manufactured by Merck Group
Sourced in Germany, Malaysia
Phosphate buffer saline (PBS) solution is a commonly used buffer in various laboratory applications. It is a balanced salt solution that maintains a stable pH and osmotic pressure. PBS solution is primarily used to dilute, suspend, or wash biological samples, such as cells or proteins, while preserving their structural and functional integrity.
Automatically generated - may contain errors
Lab products found in correlation
Milli q advantage 0.10 purification system, by Merck Group (3 mentions)
Human ace2 fc chimera, by GenScript (2 mentions)
Graphene oxide conjugated with g peg amine functionalized, by Merck Group (2 mentions)
Polyethylenimine (pei), by Merck Group (2 mentions)
Nafion, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Sodium dodecyl sulfate (sds), by Avantor (1 mentions)
Diethylpyrocarbonate water, by Thermo Fisher Scientific (1 mentions)
Anhydrous ethanol, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane, by Avantor (1 mentions)
Succinimidyl 4 n maleimidomethyl cyclohexane 1 carboxylate, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Potassium iodide, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Sodium citrate dihydrate, by Merck Group (1 mentions)
Cysteamine hydrochloride cys, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
11 protocols using phosphate buffer saline solution
1
Synthesis and Functionalization of miRNA-Coated Silver Nanoparticles
2
Electrochemical Sensor for COVID-19 Spike Protein
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All reagents used in the experiments were of analytical grade. Deionized water (resistivity ≥18 MΩ cm at 25°C) was obtained from a Milli-Q Advantage-0.10 purification system (Millipore). Human ACE2 Fc Chimera was obtained from GenScript. SP was kindly donated by Dr. Scott Hensley from the University of Pennsylvania. Graphene oxide conjugated with G-PEG amine functionalized, PEI, K3[Fe(CN)6], K4[Fe(CN)6], BSA, Nafion, EDAC, and NHS with a degree of purity ≥98% and phosphate buffer saline solution, pH = 7.4, were purchased from Sigma-Aldrich. Carbon and Ag/AgCl conductive inks of medical grade and dielectric ink were acquired from Creative Materials.
3
Electrochemical Detection of SARS-CoV-2 Spike Protein
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All reagents used in this work were of analytical grade. The deionized water (resistivity ≥18 MΩ cm at 25 °C) was obtained from a Milli-Q Advantage-0.10 purification system (Millipore). ACE2 Fc Chimera, Human was obtained from GenScript. Spike protein was kindly donated by Scott Hensley, University of Pennsylvania, Philadelphia. EDC and NHS with a degree of purity ≥98%, gold(III) chloride trihydrate (HAuCl40.3H2O) (99.99%), sodium borohydride (NaBH4) with ≥98% purity, cysteamine hydrochloride (cys) with 98% purity, phosphate buffer saline solution, pH = 7.4, and glutaraldehyde (25%, vol/vol) were purchased from Sigma-Aldrich. Graphite pencils 0.7-mm and 0.9-mm diameter under the trade name of Pentel were purchased in a local store in Philadelphia. Ag/AgCl conductive ink was acquired from Creative Materials.
Electrochemical measurements were carried out using a MULTI AUTOLAB M101 potentiostat with six channels, controlled by the NOVA 2.1 software. Cryogenic vials were used as the electrochemical cell (2.0-mL volume), each containing three electrodes. Graphite pencils (0.9 mm) were used as reference and counterelectrodes, and graphite pencils (0.7 mm) were used as the WE. The Ag/AgCl ink was painted over one of the graphites to create the reference electrode.
Electrochemical measurements were carried out using a MULTI AUTOLAB M101 potentiostat with six channels, controlled by the NOVA 2.1 software. Cryogenic vials were used as the electrochemical cell (2.0-mL volume), each containing three electrodes. Graphite pencils (0.9 mm) were used as reference and counterelectrodes, and graphite pencils (0.7 mm) were used as the WE. The Ag/AgCl ink was painted over one of the graphites to create the reference electrode.
4
Electrochemical Sensor for COVID-19 Spike Protein
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All reagents used in the experiments were of analytical grade. Deionized water (resistivity ≥18 MΩ cm at 25°C) was obtained from a Milli-Q Advantage-0.10 purification system (Millipore). Human ACE2 Fc Chimera was obtained from GenScript. SP was kindly donated by Dr. Scott Hensley from the University of Pennsylvania. Graphene oxide conjugated with G-PEG amine functionalized, PEI, K3[Fe(CN)6], K4[Fe(CN)6], BSA, Nafion, EDAC, and NHS with a degree of purity ≥98% and phosphate buffer saline solution, pH = 7.4, were purchased from Sigma-Aldrich. Carbon and Ag/AgCl conductive inks of medical grade and dielectric ink were acquired from Creative Materials.
5
Biomaterial Synthesis and Characterization
6
Myocardial Antioxidant and Oxidative Stress
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Basal myocardial antioxidant capacity and oxidative and nitrosative stress were assessed in 90 myocardial tissue samples taken from unselected patients. After the samples were pulverized in liquid N2, they were transferred to phosphate buffer saline solution, pH 7.4 (15% v/w) (Sigma, St. Louis, MO) and sonicated. After 30 min on ice, the homogenates were centrifuged at 17000g for 15 min at 4°C. The supernatants were collected and used for the analyses. Protein amounts were quantified using the DC™ protein assay (Bio-Rad, Hercules, CA).
7
Cytotoxicity Evaluation of Compounds
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Succinic acid (ACS reagent, ≥99.0%), PhenylSuccinic acid (98%), 1,10-Decanediol (98%), Scandium(III) triflate (99%), Coumarin-6 (98%), Pluronic F-68, deuterated chloroform (CDCl3), 1 mM sodium pyruvate, 1% non-essential amino acids, phosphate buffer saline solution, CFDA-AM, Neutral Red, acetic acid, 95% ethanol, ammonium chloride, Triton-X100, DAPI, H2O2, HBSS, low melting point agarose, agarose , lysis buffer base, dimethyl sulfoxide, HEPES, potassium chloride, Ethylenediaminetetraacetic acid [EDTA], Bovine Serum Albumin, sodium hydroxide, Tris base and GelRed were purchased from Sigma-Aldrich. All solvents were purchased from Fischer Scientific UK.
Cell culture reagents including MEM medium, foetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin/streptomycin, phenol red free MEM medium, 10 x trypsin (2.5%) and 0.4% v/v Trypan Blue were purchased from Gibco, Invitrogen. The human hepatocyte cell line C3A was purchased from ATCC, Manassas, VA, USA.
Cell culture reagents including MEM medium, foetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin/streptomycin, phenol red free MEM medium, 10 x trypsin (2.5%) and 0.4% v/v Trypan Blue were purchased from Gibco, Invitrogen. The human hepatocyte cell line C3A was purchased from ATCC, Manassas, VA, USA.
8
Carbomer-Agarose Hydrogel for Ibuprofen
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Carbomer 974P (CAS 151687-96-6) with high molecular weight (about 1 MDa) was provided by Fagron (The Netherlands), triethylamine (TEA, CAS 121-44-8) with high purity was purchased from Fluka (Switzerland), while propylene glycol (CAS 504-63-2) and glycerol (CAS 56-81-5) were provided by Sigma-Aldrich (Germany). Phosphate Buffer Saline solution (PBS), purchased from Sigma-Aldrich (Germany), was used as solvent. Deuterated PBS was used for spectroscopic analysis in order to avoid overlapping of 1 H signals. The other polymer involved in the reaction is agarose (CAS 9012-36-6), purchased from Invitrogen (USA) and having a molecular weight of about 300 kDa. Lastly, ibuprofen sodium salt (IBU, CAS 31121-93-4) was provided by Sigma-Aldrich (Germany). All materials were used as received.
9
Biomarker Detection Protocol on ITO Glass
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ITO coated glass sheets (resistivity of ~ 20 Ω/sqm) were procured from Shilpa Enterprises, India. Abcam provided CEA, CYFRA 21-1 antigens and monoclonal anti-IgG CEA and anti-IgG CYFRA 21-1 antibodies. Melamine, triazine, phosphate buffer saline (PBS) solution, bovine serum albumin (BSA) and all the other chemicals were purchased from Sigma Aldrich. All the experiments were carried out with ultrapure water (18.11 M Ω cm).
10
Dipeptide-Graphene Oxide Interactions
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Phe-Phe (FF) and Tyr-Tyr (YY) dipeptides were obtained from CASLO ApS (Denmark). 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), copper sulfate and Thioflavin-T (Th-T) were purchased from Sigma Aldrich (Italy). Ultrapure Millipore water (18.2 mΩ cm at 25°C) was used to prepare all aqueous solutions. GO was purchased from Graphenea Inc., (United States). Small unilamellar vesicles (SUVs) were prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and rhodamine-labeled 1,2-dihexadecanoyl-snglycero-3-(Rhod-DHPE) purchased from Avanti Polar Lipids (Alabaster, AL, United States). Phosphate buffer saline (PBS) solution (0.01 M phosphate buffer containing 0.003 M KCl and 0.14 M NaCl, pH 7.4) was prepared from tablets (Sigma). Dulbecco’s modified eagle medium (DMEM)-F12, RPMI 16-40 medium, fetal bovine serum (FBS), streptomycin and L-glutamine, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Aldrich.
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