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Stellaris 5 confocal system

Manufactured by Leica
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The STELLARIS 5 is a high-performance confocal microscopy system designed for advanced imaging applications. It utilizes a multiline laser combiner to provide up to five laser lines for excitation, enabling the simultaneous acquisition of multiple fluorophores. The system features a galvanometric scanner and a high-sensitivity photomultiplier tube (PMT) detector, providing fast and efficient image acquisition.

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Lab products found in correlation

Application suite x las x, by Leica (2 mentions) Macro lens and camera, by Sony (2 mentions) Aperio at2 system, by Leica (1 mentions) Zen blue v 3, by Zeiss (1 mentions) Lsm980 system, by Zeiss (1 mentions)

4 protocols using stellaris 5 confocal system

1

Multimodal Imaging Techniques for Comprehensive Sample Analysis

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Gross images were taken with a Sony Macro Lens and camera. Figure 1A was created with BioRender.com. IHC images were captured using Leica STELLARIS 5 confocal system (using the 10x objective) with Leica Application Suite X (LAS X) at the CHP Rangos imaging core facility. Brightfield imaging was obtained using the Thermo Fisher EVOS M7000 system (using the 10x and 20x objectives) with EVOS M7000 Software revision 2.0.2094.0. Whole H&E slides were scanned using the Leica Aperio AT2 system (using the 40x objective) and subsequently evaluated using Aperio ImageScope 12.4.3. Images were optimized using Adobe Photoshop (version 23.3.1). Figures were compiled using Adobe Illustrator (version 26.2.1).
Kalsi R.S., Kreger A.M., Saleh M., Yoshida S., Sharma K., Fusco J., Saloman J.L., Zhang T., Thomas M., Sehrawat A., Wang Y., Reif J., Mills J., Raad S., Zengin B., Gomez A., Singhi A., Tadros S., Slivka A., Esni F., Prasadan K, & Gittes G. (2023). Chemical pancreatectomy in non-human primates ablates the acini and ducts and enhances beta-cell function. Research Square.
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2

High-Resolution Confocal Imaging Protocols

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All images were acquired using a Leica Stellaris 5 confocal system. Images were taken with a 60x, 1.40 NA oil immersion objective. Imaging parameters were minimally adjusted between images to achieve an image that utilizes the full pixel intensity range without oversaturating pixels.
Moreno-Sanchez A., Vasserman A.N., Jang H., Hina B.W., von Reyn C.R, & Ausborn J. (2024). Morphology and synapse topography optimize linear encoding of synapse numbers in Drosophila looming responsive descending neurons. bioRxiv.
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3

Comprehensive Imaging Protocol for Biological Samples

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Gross images were taken with a Sony Macro Lens and camera. Figure 1a was created with https://www.biorender.com/. IHC images were captured using Leica STELLARIS 5 confocal system (using the 10 × objective) with Leica Application Suite X (LAS X) at the CHP Rangos imaging core facility. Brightfield imaging was obtained using the Thermo Fisher EVOS M7000 system (using the 10 × and 20 × objectives) with EVOS M7000 Software revision 2.0.2094.0. Whole H&E slides were scanned using the Leica Aperio AT2 system (using the 40 × objective) and subsequently evaluated using Aperio ImageScope 12.4.3. Images were optimized using Adobe Photoshop (version 23.3.1). Figures were compiled using Adobe Illustrator (version 26.2.1).
Kalsi R.S., Kreger A.M., Saleh M., Yoshida S., Sharma K., Fusco J., Saloman J.L., Zhang T., Thomas M., Sehrawat A., Wang Y., Reif J., Mills J., Raad S., Zengin B., Gomez A., Singhi A., Tadros S., Slivka A., Esni F., Prasadan K, & Gittes G. (2023). Chemical pancreatectomy in non-human primates ablates the acini and ducts and enhances beta-cell function. Scientific Reports, 13, 9113.
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4

Imaging and Microscope Techniques for Cell Biology

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Most of the images were acquired at room temperature using a Zeiss LSM980 system, equipped with two PMT and one GaAsPand, a ×40 W objective (C-Apochromat ×40/1.1 W Corr M27, Zeiss); same microscope, but a C-Apochromat ×25/1.515 oil immersion objective was used for the TFM experiments. Camera, filter wheels and shutters were controlled by Zeiss’s ZEN Blue v.3.0. Images in Extended Data Fig. 3 and Extended Data Fig. 6 were acquired with a Leica Stellaris 5 confocal system by using a ×63/1.4 oil immersion objective with ×1 or ×0.75 optical zoom, respectively. Images in Extended Data Fig. 7b were acquired in a Leica Thunder by using a HC PL APO ×20/0.80 PH2 objective. Camera, filter wheels and shutters were controlled by built-in Leica softwares.
Marchant C.L., Malmi-Kakkada A.N., Espina J.A, & Barriga E.H. (2022). Cell clusters softening triggers collective cell migration in vivo. Nature Materials, 21(11), 1314-1323.
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