Previously reported protocols were applied (5 (link), 6 (link)). To identify podocytes, we used a combination of WT1 (Agilent Technologies; IS05530-2) and DACH1 (Sigma-Aldrich; HPA012672) (32 (link)) as primary antibodies; Alexa-Fluor 488, -555, and/or -647 as secondary antibodies (Invitrogen; A21202, A31572, A31571, and A31573, respectively) depending on the experiment; and a DNA marker to identify single nuclei — either DAPI (Sigma-Aldrich; D9542) or DRAQ5 (Abcam; ab108410). Optical images were obtained using inverted laser confocal microscopes (Nikon and LSM800, Zeiss), stored in 1024 × 1024 pixel frames. Each image contained 1 glomerulus.
A31573
Manufactured by Merck Group
Sourced in United States, Germany
A31573 is a piece of lab equipment manufactured by Merck Group. It is designed for general laboratory use. The core function of this product is to facilitate common laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
A31571, by Merck Group (4 mentions)
A21202, by Thermo Fisher Scientific (3 mentions)
D9542, by Merck Group (2 mentions)
A21206, by Merck Group (2 mentions)
A10040, by Merck Group (2 mentions)
Leica sp8, by Leica camera (2 mentions)
Ab96051, by Abcam (2 mentions)
A21206, by Thermo Fisher Scientific (2 mentions)
A21202, by Merck Group (2 mentions)
Sa5 35571, by Thermo Fisher Scientific (2 mentions)
Sc 50509, by Proteintech (2 mentions)
A31572, by Merck Group (2 mentions)
A31570, by Merck Group (2 mentions)
Anti atrx, by Santa Cruz Biotechnology (2 mentions)
Ab108410, by Abcam (1 mentions)
A31572, by Thermo Fisher Scientific (1 mentions)
Hpa012672, by Merck Group (1 mentions)
D9542, by Abcam (1 mentions)
Lsm 800, by Zeiss (1 mentions)
Ab53444, by Abcam (1 mentions)
5 protocols using a31573
1
Podocyte Identification Protocol
2
Immunohistochemical Analysis of Cardiac Tissue
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The SV tissues and frozen sections were permeabilized in 0.5% Triton X-100 (Sigma-Aldrich) for 1 hour and immunoblocked with 1% fish gelatin solution (G7765, Sigma) for another 1 hour. The specimens were incubated with different primary antibodies: ZO-1 (1 : 1000; 61-7300, Thermo Fisher), Cx26 (1 : 200; LS-B6429-50, Life Technologies), Cx43 (1 : 400; Ab135763, Abcam), laminin (1 : 200; Ab30320, Abcam), collagen IV (1 : 200; Ab6586, Abcam), alpha-smooth muscle actin (α-SMA, 1 : 200; Ab5694, Abcam), and CD68 (1 : 100; Ab53444, Abcam), diluted in 1% bovine serum albumin- (BSA-) PBS, respectively, at 4°C overnight. The next day, cells were incubated with secondary antibodies (Invitrogen, A21202, A21206, A31571, A31573, and A10040) along with DAPI (D9542, Sigma-Aldrich, USA) at room temperature for 1 h. Coverslips were then mounted, and the samples were observed under a laser scanning confocal microscope (Leica SP8; Leica, Germany).
3
Antibody Validation for Immunofluorescence and Western Blotting
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The following antibodies were used for immunofluorescence or western blotting: Primary rabbit polyclonal: anti-actin (Sigma-Aldrich, A5060), anti-Daxx (Upstate, 07–471), anti-ATRX (Santa Cruz, H300), anti-PML (Bethyl Laboratories, A301-167A; Jena Biosciences, ABD-030), anti-Sp100 (GeneTex, GTX131569), anti-Mx1 (Santa Cruz, sc-50509; ProteinTech, 13750-1-AP), anti-ISG15 (ProteinTech, 15981-1-AP), anti-ISG54 (IFIT2, proteinTech, 12604-1-AP), and anti-histone H3 (abcam, ab1791). Primary mouse monoclonal: anti-HIRA (Millipore, 04–1488), anti-ICP0 (11060, [114 (link)]), anti-ICP4 (58s, [115 (link)]), anti-VP5 (DM165, [116 (link)]) anti-UL42 (Z1F11; [117 (link)]), and anti-PML (abcam, ab96051). Primary antibodies were detected using the following secondary antibodies: DyLight-680 or -800 conjugated goat anti-rabbit or -mouse (Thermo; 35568 and SA5-35571), Alexa -488, -555, or -647 conjugated donkey anti-rabbit or -mouse (Invitrogen; A21206, A21202, A31572, A31570, A31573, A31571), or HRP conjugated goat anti-mouse (Sigma-Aldrich, A4416).
4
Immunohistochemical Analysis of Cochlear Cells
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After culturing or cryosectioning, the cochlear explants or tissue sections were fixed with 4% paraformaldehyde, permeabilized with 1% Triton X-100 (Sigma-Aldrich, T8787) in PBS (Thermo Fisher Scientific, 10,010,023), and immersed in blocking solution (0.1% Triton X-100, 8% donkey serum (Sigma-Aldrich, D9663), 1% bovine serum albumin (Sigma-Aldrich, A1933), 0.02% sodium azide in PBS) at room temperature for 1 h. After blocking, the samples were incubated with primary antibodies against TUBB3/Tuj1 (1:1,000 dilution; Neuromics, MO15013), LC3B (1:400 dilution; Cell Signaling Technology, 3868), SQSTM1 (1:500 dilution; Abcam, ab91526), and RBFOX3/NeuN (1:500; Cell Signaling Technology,12,943), 4-HNE (1:500; Abcam, ab48506), cleaved-CASP3 (1:400 dilution; Cell Signaling Technology, 9664), PRDX1 (1:500 dilution; GeneTex, GTX101705), or MYO7A (1:800 dilution; Proteus Biosciences, 25–6790) diluted in blocking solution at 4°C overnight. The next day, cells were incubated with secondary antibodies (Invitrogen, A21202, A21206, A31571, A31573, A10040) along with DAPI (Sigma-Aldrich, D9542) at room temperature for 1 h. Coverslips were then mounted and the samples were observed under a laser scanning confocal microscope (Leica SP8; Leica, Germany).
5
Antibody panel for protein detection
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The following antibodies were used for immunofluorescence or western blotting: Primary rabbit polyclonal: anti-actin (Sigma-Aldrich, A5060), anti-Daxx (Upstate, 07–471), anti-ATRX (Santa Cruz, H300), anti-PML (Bethyl Laboratories, A301-167A; Jena Biosciences, ABD-030), anti-Sp100 (GeneTex, GTX131569), anti-SUMO2/3 (Abcam, ab22654), anti-Mx1 (Santa Cruz, sc-50509;ProteinTech, 13750-1-AP), anti-ISG15 (ProteinTech, 15981-1-AP), and anti-ISG54 (IFIT2, proteinTech, 12604-1-AP). Primary mouse monoclonal: anti-ICP0 (11060, [92 (link)]), anti-ICP4 (58s, [93 (link)]), anti-VP5 (DM165, [94 (link)]), anti-SUMO2/3 (Abcam, ab81371), anti-PML (abcam, ab96051), anti-IFI16 (abcam, ab55328; Santa Cruz, sc-8023). Primary antibodies were detected using the following secondary antibodies: DyLight-680 or -800 conjugated anti-rabbit or -mouse (Thermo; 35568 and SA5-35571), Alexa -488, -555, or -647 conjugated anti-rabbit, or -mouse (Invitrogen; A21206, A21202, A31572, A31570, A31573, A31571), HRP conjugated anti-mouse (Sigma-Aldrich, A4416).
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