1650 uv vis spectrophotometer

Spectrophotometric measurements were carried out in 10 mm matched glass cells in the range of 200–1100 nm using a Shimadzu (Kyoto, Japan) 1650 UV-vis spectrophotometer that is controlled by a UV-probe 2.5 software. Eppendorf 20–200 μL and 100–1000 μL vary-pipettes (Westbury, NY, USA) and a calibrated EDT pH-mV meter (Dover Kent, UK) were used.
A flat bottom 96-micro-well plate was used for colour absorbance measurements using an Hp-Deskjet F4200 all in one printer-scanner-copier. A conventional HP X7H29EA#ABV Notebook running under Windows 10 Home 64 bit was used for data treatment and analysis. Photoshop CS6 was used for digital image processing and ImageJ2x software 2.1.4.7 was used for RGB channel intensities calculations. Then, for each image, the RGB colour absorbance (A_R, A_G, A_B) was calculated.^{35,36 (link)}

One gram of microsponge-loaded gel was accurately weighed and dissolved in methanol, filtered and volume was made to 100 ml with methanol. The drug content was determined by diluting the resulting solution for 10 times with methanol and measuring the absorbance at 260 nm using Shimadzu-1650 UV/Vis spectrophotometer[15 ].