Histidine antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Histidine antibody is a laboratory tool used to detect and quantify the presence of histidine, an amino acid, in samples. It functions by specifically binding to histidine molecules, allowing for their identification and measurement. The antibody's core purpose is to serve as a reagent for analytical and research applications involving the analysis of histidine content.

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Lab products found in correlation

1 methyl 4 phenyl 1 2 3 6 tetrahydropyridine mptp, by Merck Group (1 mentions) Pep 1 peptides, by Peptron (1 mentions) Pd 10 column, by Cytiva (1 mentions) Ni2 nitrilotriacetic acid sepharose superflow, by Qiagen (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)

Close spelling variants of this product

Histidine (5 citations)

2 protocols using histidine antibody

Western Blotting of PEP-1-GLRX1 in SH-SY5Y Cells

Cited 1 time

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All antibodies used in Western blotting were obtained from Cell Signaling Technology (Beverly, MA, USA) except histidine antibody (Santa Cruz Biotechnology; Santa Cruz, CA, USA). PEP-1-GLRX1 protein was purified as previously described [27 (link),33 (link)]. PEP-1 peptides were purchased from PEPTRON (Daejeon, Korea). Methyl-4-phenylpyridinium (MPP⁺) and 1-methyl-4-phenyl-1,2,3,6-tetrahydro pyridine (MPTP) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Human neuroblastoma cells (SH-SY5Y) were cultured in MEM with 15% FBS, 2 mM L-glutamine, and 100 µg/mL gentamicin at 37 °C.

Choi Y.J., Kim D.W., Shin M.J., Yeo H.J., Yeo E.J., Lee L.R., Song Y., Kim D.S., Han K.H., Park J., Lee K.W., Park J.K., Eum W.S, & Choi S.Y. (2021). PEP-1-GLRX1 Reduces Dopaminergic Neuronal Cell Loss by Modulating MAPK and Apoptosis Signaling in Parkinson’s Disease. Molecules, 26(11), 3329.

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Hippocampal HT-22 Cell Culture Protocol

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Ni²⁺-nitrilotriacetic acid Sepharose Superflow was purchased from Qiagen (Valencia, CA, USA). PD-10 columns were purchased from Amersham (Brauncschweig, Germany). Fetal bovine serum (FBS) and antibiotics (streptomycin and penicillin) were obtained from Gibco BRL (Grand Island, NY, USA). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Lonza/BioWhittaker (Walkersville, MD, USA). 2′,7′-Dichlorofluorescein diacetate (DCF-DA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Histidine antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The indicated antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Unless otherwise stated, all other agents were of the highest grade available.
Mouse hippocampal HT-22 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% Fetal bovine serum (FBS) and antibiotics (100 μg/mL streptomycin, 100 μg /mL penicillin) at 37°C in a humidity chamber with 5% CO₂ and 95% air.

Yeo E.J., Eum W.S., Yeo H.J., Choi Y.J., Sohn E.J., Kwon H.J., Kim D.W., Kim D.S., Cho S.W., Park J., Han K.H., Lee K.W., Park J.K., Shin M.J, & Choi S.Y. (2021). Protective Role of Transduced Tat-Thioredoxin1 (Trx1) against Oxidative Stress-Induced Neuronal Cell Death via ASK1-MAPK Signal Pathway. Biomolecules & Therapeutics, 29(3), 321-330.

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