Histidine antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Histidine antibody is a laboratory tool used to detect and quantify the presence of histidine, an amino acid, in samples. It functions by specifically binding to histidine molecules, allowing for their identification and measurement. The antibody's core purpose is to serve as a reagent for analytical and research applications involving the analysis of histidine content.

Automatically generated - may contain errors

Lab products found in correlation

1 methyl 4 phenyl 1 2 3 6 tetrahydropyridine mptp, by Merck Group (1 mentions) Pep 1 peptides, by Peptron (1 mentions) Pd 10 column, by Cytiva (1 mentions) Ni2 nitrilotriacetic acid sepharose superflow, by Qiagen (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)

Spelling variants of this product

Histidine (5 citations)

2 protocols using histidine antibody

Western Blotting of PEP-1-GLRX1 in SH-SY5Y Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All antibodies used in Western blotting were obtained from Cell Signaling Technology (Beverly, MA, USA) except histidine antibody (Santa Cruz Biotechnology; Santa Cruz, CA, USA). PEP-1-GLRX1 protein was purified as previously described [27 (link),33 (link)]. PEP-1 peptides were purchased from PEPTRON (Daejeon, Korea). Methyl-4-phenylpyridinium (MPP⁺) and 1-methyl-4-phenyl-1,2,3,6-tetrahydro pyridine (MPTP) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Human neuroblastoma cells (SH-SY5Y) were cultured in MEM with 15% FBS, 2 mM L-glutamine, and 100 µg/mL gentamicin at 37 °C.

Choi Y.J., Kim D.W., Shin M.J., Yeo H.J., Yeo E.J., Lee L.R., Song Y., Kim D.S., Han K.H., Park J., Lee K.W., Park J.K., Eum W.S, & Choi S.Y. (2021). PEP-1-GLRX1 Reduces Dopaminergic Neuronal Cell Loss by Modulating MAPK and Apoptosis Signaling in Parkinson’s Disease. Molecules, 26(11), 3329.

+ Open protocol

+ Expand

Hippocampal HT-22 Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ni²⁺-nitrilotriacetic acid Sepharose Superflow was purchased from Qiagen (Valencia, CA, USA). PD-10 columns were purchased from Amersham (Brauncschweig, Germany). Fetal bovine serum (FBS) and antibiotics (streptomycin and penicillin) were obtained from Gibco BRL (Grand Island, NY, USA). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Lonza/BioWhittaker (Walkersville, MD, USA). 2′,7′-Dichlorofluorescein diacetate (DCF-DA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Histidine antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The indicated antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Unless otherwise stated, all other agents were of the highest grade available.
Mouse hippocampal HT-22 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% Fetal bovine serum (FBS) and antibiotics (100 μg/mL streptomycin, 100 μg /mL penicillin) at 37°C in a humidity chamber with 5% CO₂ and 95% air.

Yeo E.J., Eum W.S., Yeo H.J., Choi Y.J., Sohn E.J., Kwon H.J., Kim D.W., Kim D.S., Cho S.W., Park J., Han K.H., Lee K.W., Park J.K., Shin M.J, & Choi S.Y. (2021). Protective Role of Transduced Tat-Thioredoxin1 (Trx1) against Oxidative Stress-Induced Neuronal Cell Death via ASK1-MAPK Signal Pathway. Biomolecules & Therapeutics, 29(3), 321-330.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!