Rhodamine phalloidin stain

Pegylated human recombinant Arginase I cobalt [HuArgI (Co)-PEG5000] (Pegzilarginase) was a gift from Aeaglea BioTherapeutics (Texas, USA). The constitutively active RhoA (CA-RhoA) and the empty vector plasmid (pcDNA3.1) constructs were also gifts from Dr. Yamaguchi Hideki. Chloroquine, rapamycin and L-citrulline were purchased from Sigma-Aldrich (Darmstadt, Germany). Cyto-ID autophagy detection kit was obtained from Enzo Life Sciences (New York, USA). RhoA/Rac1/Cdc42 Activation Assay Combo Kit was from Cell BioLabs (Sand Diego, CA, USA). Actin and vinculin primary antibodies were purchased from Abcam (Cambridge, UK). Rabbit polyclonal anti-LC3 antibody was obtained from Cell Signaling (Cell Signaling Technology Inc., US). Fluorescent secondary Alexa Fluor 488-green as well as Rhodamine phalloidin stain were obtained from Invitrogen (Massachusetts, USA). Hiperfect transfection reagent, luciferase GL2 and human Flexi Tubes siRNA for RhoA were bought from Qiagen (Hilden, Germany). Lipofectamine LTX was from Waltham (Massachusetts, USA) and crystal violet was from SCP Science (Quebec, Canada).

Cdc42 and RhoA biosensors were kind gifts from Dr. Louis Hodgson (Albert Einstein College of Medicine). RhoA/Rac1/Cdc42 Activation Assay Combo Kit was purchased from Cell BioLabs (Sand Diego, CA, USA). QCM Gelatin Invadopodia Assay was obtained from Millipore (Massachusetts, USA). Primary antibodies against Cdc42, StarD13, actin, vinculin, Arp2, cortactin (Mouse and Rabbit) and TKS5 antibodies were obtained from Abcam (Cambridge, UK). Primary mouse anti-TKS4 was obtained from Merck Millipore. Primary mouse anti-StarD13 and rabbit anti-Cdc42 were obtained from Santa Cruz Biotech. HRP-conjugated secondary antibodies were obtained from Promega (Wisconsin, USA). Fluorescent secondary antibodies Alexa Fluor 488-green and Alexa Fluor 594-red as well as Rhodamine-phalloidin stain were purchased from Invitrogen (Massachusetts, USA). DAPI stain, and cell proliferation reagent were acquired from Roche Diagnostics (Roche Ltd, Mannheim, Germany). Hiperfect transfection reagent, luciferase and human Flexi Tubes siRNA for luciferase, StarD13, Cdc42 and RhoA were obtained from Qiagen (Hilden, Germany). Lipofectamine LTX was from Waltham (Massachusetts, USA) and crystal violet was from SCP Science (Quebec, Canada).