Human orexin a

CRH, Dulbecco’s Modified Eagle’s Medium (DMEM), and DMEM high glucose were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Recombinant human BMP-4 was purchased from R&D Systems Inc. (Minneapolis, MN, USA), and human orexin A was purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS) and penicillin/streptomycin solution (PS) were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Horse serum (HS) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Recombinant human BMP-4 was obtained from R&D Systems Inc. (Minneapolis, MN, USA), and human orexin A was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The PC12 rat pheochromocytoma cell line was obtained from RIKEN (Saitama, Japan) and was cultured in DMEM supplemented with 10% FBS, 10% HS and 1% PS with 5% CO₂ at 37 °C. The cell cultures were passaged at nearly 80% confluence.

Forskolin (FSK) was purchased from Sigma-Aldrich Co. Ltd. (St. Louis, MO, USA) and recombinant human BMP-6 was purchased from R&D Systems Inc. (Minneapolis, MN, USA). Human orexin A was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The H295R human adrenocortical cell line was cultured in DMEM/F12 containing 10% FBS supplemented with insulin-transferrin-selenium (ITS-G; Thermo Fisher Scientific, Waltham, MA, USA) with 5% CO₂ at 37 °C [27 (link)].