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Em scd500

Manufactured by JEOL
Sourced in Japan, Germany

The EM SCD500 is a sputter coater designed for sample preparation in electron microscopy. It deposits a thin, uniform conductive coating on a specimen, which is necessary for high-resolution imaging in scanning electron microscopes. The EM SCD500 provides a controlled and consistent sputtering process to enhance the quality of electron microscopy samples.

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3 protocols using em scd500

1

SEM Imaging of Platinum-Coated MPAs

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MPAs of various geometries were affixed onto a sticky tape, sputter-coated with 4-nm-thickness platinum (Leica EM SCD500), and examined by SEM (JSM-6701F, JEOL, Japan).
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2

Epipharynx and Hypopharynx Dissection Procedure

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The epipharynx and hypopharynx were dissected from the preoral chamber as described in Koch and Edgecombe (2008) (link) in one to four adult male or female individuals per species. Multifocus images of the sclerotized parts of the epipharynx and hypopharynx were obtained with a Nikon SMZ25 stereomicroscope equipped with a Nikon DS-F2.5 camera using NIS-Elements Microscope Imaging Software with an PageBreakExtended Depth of Focus (EDF) patch. For scanning electron microscopy (SEM), the specimens were: (1) cleaned in an ultrasonic bath (50–60 Hz) for 5 to 10 seconds (maximum), occasionally in a solution of 15% hydrogen peroxide for 2 hours; (2) dehydrated in an ascending alcohol series (70%, 80%, 90%, 96% EtOH, 2 × 10-15 min each); (3) air dried overnight (or covered with HMDS) or critical point dried (Leica 300 CPD). Specimens were mounted on aluminium stubs equipped with a sticky aluminium tape, glued with conductive silver, coated with platinum (Leica EM SCD500) and studied with a JEOL JSM 6610-LV at an accelerating voltage of 15 kV. Figures were processed with Adobe Photoshop CS6 and assembled in Adobe InDesign CS6.
Terminology follows Koch and Edgecombe (2008) (link).
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3

Microcapsule Morphology and Physicochemical Characterization

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The morphology of the microcapsules was determined in seven days of housing at -20 ºC. For this, samples were xed with a double-sided carbon tape, spray coated with gold (Leica, model EM SCD 500, Wetzlar, Germany) and scanned by a electron microscopy (SEM) Jeol®, model JSM - 6610LV (Jeol®, Tokyo, Japan), in high vacuum mode to examine the morphology of the surface. The average particle size was determined with the aid of the Image J software, starting on 50 measurements.
Water activity (aw) was measured by a direct method, at 25°C, using the Labtouch-aW analyzer (Novasina, Neuheimstrasse, Switzerland).
The moisture present in the microcapsule was determined gravimetrically by oven drying, according to the guidelines of the Association of O cial Analytical Chemists (AOAC, 2012), according to method No. 990.20.
Color analyses were determined using a colorimeter (Minolta Chroma Meter CR-300, Osaka, Japan), with a CIE 'L * a * b *' reading system, proposed by the Commission Internationale de I'Eclairage (CIE).
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