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Pinene

Manufactured by Merck Group
Sourced in United States

Pinene is a laboratory instrument used for the detection and analysis of pinene, a terpene compound found in various plant species. The core function of Pinene is to accurately measure and quantify the concentration of pinene in a given sample. It utilizes advanced analytical techniques to provide reliable and precise data for researchers and scientists working in fields such as plant chemistry, essential oil analysis, and environmental monitoring.

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3 protocols using pinene

1

Analytical Standards for Terpenes and Esters

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Authentic chemical standards (>95% purity) of -pinene (97%), -pinene (97%), (z)-ocimene (>90%), undecane (>99%), allo-ocimene (80% technical grade), ethyl benzoate (>99%) and -caryophyllene (>80%) were purchased from Sigma Aldrich. The remaining chemicals for which no standards are commercially available were identified through NIST spectral library 14.
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2

Yeast Hexokinase Enzymatic Assay

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All buffers, reagents and cofactors (for example, bis-tris propane, glucose, limonene, pinene, sabinene, FBP, ATP, NAD(P)+, coenzyme A and so on) were purchased from Sigma-Aldrich. Yeast Hex (H6380) was from Sigma. Hexanes, isopropyl myristate and nonane were from ARCOS and were the highest grade available. Liquid and solid media for growth of E. coli was from Fisher Scientific (BD Difco).
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3

Curcumin-Loaded Nanoemulsion Formulation

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The following components were used to prepare the blank and curcumin-loaded samples (nanoemulsions): medium chain triglycerides (MCT)—Mygliol 812 (Fagron, Rotterdam, The Netherlands), polysorbate 80 (Across Organics, Geel, Belgium), soybean lecithin with 70% of phosphatidylcholine—Lipoid S 75 (Lipoid, Ludwigshafen, Germany), 1,8-cineole (eucalyptol; Sigma Aldrich, St. Louis, MO, USA), pinene (Sigma Aldrich, St. Louis, MO, USA), highly purified water (GenPure, TKA Wasseranfbereitungssysteme GmbH, Niederelbert, Germany). curcumin (Sigma Aldrich, St. Louis, MO, USA) was used as a model active ingredient. All other substances and reagents used in the experimental work were of pharmacopoeial (Ph. Eur.) or HPLC quality, and were used without further purification.
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