Cell number was determined using a CASY cell counter and analyzer (Roche, Germany) at each passage and long-term growth kinetics in vitro was assessed by determining cumulative population doublings (CPDs). The number of population doublings (PDs) was calculated using the formula: PD = log(Xb/Xa)·3.3, where Xa represents the initial cell number, Xb represents the cell harvest number, and 3.3 is a coefficient. PD of each passage was calculated and added to the PD of the previous passage level to obtain the CPD.
Casy cell counter and analyzer
Manufactured by Roche
Sourced in Germany, Switzerland
The CASY cell counter and analyzer is a laboratory instrument used to count and analyze cells. It provides accurate cell counts and measures various cell parameters, such as size and viability. The CASY system utilizes electrical impedance technology to perform these measurements.
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Lab products found in correlation
6 protocols using casy cell counter and analyzer
1
Cell growth kinetics assessment
2
Cell Viability Assay via Cell Counter
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After performing a single treatment (either 2.3. isolated 5 s CPP application; or 2.4. exposure to chemotherapeutic agents; or 2.5. combined treatment), cells were incubated over a 120 h period. The number of living cells was determined using the CASY cell counter and analyzer (Roche Applied Science, Mannheim, Germany). The population doublings were calculated using the following formula: PDL = PDL0 + 3.322 (logCf − logCi).
3
Cell Proliferation Assay in ACH-3P Cells
4
Promastigote Cell Density Monitoring
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Promastigotes were grown in complete M199 medium in 25 cm2 cell culture flasks. Cell density was monitored using a CASY® Cell Counter and Analyzer (Roche, Mannheim, Germany).
5
Comprehensive Murine Bone and Spleen Sampling
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Tibia, femur, pelvis, radius together with humerus were collected from every mouse. Additionally, we harvested sternum, calvarium, and the vertebrae (cervical vertebrae C1–sacral vertebrae S5). Bones were cleaned and crushed in MACS buffer (PBS + 1% FCS + 2 mM EDTA) using a mortar and pestle. BM cell suspensions were filtered through a 40-μm cell strainer to remove bone debris. Single splenocyte suspensions were prepared by crushing the spleen through a 40-μm cell strainer with the plunger of a syringe. For several LCMV experiments, whole spleen and BM cells were enriched for CD8+ T cells with CD8α microbeads (Miltenyi Biotec) and MACS LS columns (Miltenyi Biotec). Erythrocytes were lysed with red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 127 mM EDTA). White blood cells were counted with CASY Cell Counter and Analyzer (Roche).
6
Immortalized Human Renal Proximal Tubular Cells
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The experiments were carried out with the HK-2 cell line. HK-2 cells, which are human renal proximal tubular epithelial cells immortalized by transduction with human papilloma virus 16 E6/E7 genes, were obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK). The HK-2 cells were cultured in a DMEM/F12 (1:1) medium supplemented with 5% FBS, 2 mM L-glutamine, 10 ng/ mL epidermal growth factor (EGF), a 10 μg/mL insulin-transferrin-selenium (ITS) solution, 50 μg/mL penicillin and 50 μg/mL streptomycin (all reagents from Life Technologies, Grand Island, NY, USA). The cells were maintained under standard cell culture conditions at 37 °C in a humidified incubator in an atmosphere of 5% CO 2 -95% air. A CASY Cell Counter and Analyzer (Roche, Basel, Switzerland) were used for basic quality control of the cell culture system and for evaluating cell numbers. HK-2 cells in the maximum range of 20 passages and in an exponential growth phase were used for this study.
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