VEGF-Aflox/flox mice were anaesthetized, and 1ul (10 ng) of either recombinant (r) VEGF-A (Prospec, Rehovot, Israel) or ultrapure DNase/RNase-free distilled water (vehicle) (Life Technologies, Carlsbad, CA, USA) were injected into the stromal layer of the cornea using a gas-powered microinjection system as described previously15 (link),28 (link). Immediately following the injection, the mice were inoculated with 200 PFUs of SC16 ICP0-Cre. On day 2 pi, the mice were injected again with r-VEGF-A or vehicle, and on day 7 pi, the mice were euthanized to harvest corneas for whole mount.
Ultrapure dnase rnase free distilled water vehicle
Manufactured by Thermo Fisher Scientific
Sourced in Israel
Ultrapure DNase/RNase-free distilled water (vehicle) is a high-quality laboratory-grade water that is free from detectable levels of DNase and RNase enzymes. It is designed for use in sensitive molecular biology applications where the presence of these enzymes could interfere with experimental results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ultrapure dnase rnase free distilled water vehicle
1
VEGF-A Modulation of Corneal Immunity
2
VEGF-A Modulation of Corneal Immunity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
VEGF-Aflox/flox mice were anaesthetized, and 1ul (10 ng) of either recombinant (r) VEGF-A (Prospec, Rehovot, Israel) or ultrapure DNase/RNase-free distilled water (vehicle) (Life Technologies, Carlsbad, CA, USA) were injected into the stromal layer of the cornea using a gas-powered microinjection system as described previously15 (link),28 (link). Immediately following the injection, the mice were inoculated with 200 PFUs of SC16 ICP0-Cre. On day 2 pi, the mice were injected again with r-VEGF-A or vehicle, and on day 7 pi, the mice were euthanized to harvest corneas for whole mount.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!