The OptoMotry system from CerebralMechanics (Lethbridge, Canada) was used to measure the OKR with the method described previously [51 (link),52 (link)]. A virtual cylinder comprising a vertical sine wave grating was projected in three-dimensional (3D) coordinate space on four computer monitors (Dell 1703FP, Bern, Switzerland) facing a 13-cm-high platform above a mirrored floor under a likewise mirrored lid. The light intensity measured at the center of the platform was 5.6 × 1013 photons cm−2 s−1. During testing, the mice stood unrestrained on the platform tracking the 3D pattern with a reflexive head movement that was recorded with a video camera (DCR-HC26, Sony, Bern, Switzerland). The movements of the mice on the platform were followed by two independent experimenters. The spatial frequency of the test grating was changed in steps until the highest spatial tracking frequency was identified as the threshold (visual acuity). A minimum spatial acuity of 0.003 cycles per degree was assigned to all mice with no detectable OKR reflex.
Dcr hc26
Manufactured by Sony
Sourced in Switzerland
The DCR-HC26 is a compact digital video camera manufactured by Sony. It features a 2.5-inch LCD screen, a built-in microphone, and the ability to record video in the DV format.
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Lab products found in correlation
2 protocols using dcr hc26
1
Measuring Visual Acuity in Mice Using OptoMotry
2
Measuring Visual Acuity in Mice Using OptoMotry
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The OptoMotry system from CerebralMechanics (Lethbridge, Alberta, Canada) was used to measure the OKR with the method described previously [47,80]. A virtual cylinder comprising a vertical sine wave grating was projected in 3-D coordinate space on four computer monitors (Dell 1703FP) facing a 13-cm-high platform above a mirrored floor under a likewise mirrored lid. The light intensity measured at the centre of the platform was 5.6 1013 photons cm-2 s-1, sufficient to activate Opto-mGluR6. During testing, the mice stood unrestrained on the platform, tracking the 3-D pattern with a reflexive head movement that was recorded with a video camera (DCR-HC26, Sony). The movements of the mice on the platform were followed by two independent experimenters with a crosshair superimposed on the video image to center the rotation of the virtual cylinder with the x-y positional coordinates of the crosshair at the head of the mouse. The spatial frequency of the test grating was changed in steps until the highest spatial tracking frequency was identified as the threshold (visual acuity).
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