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Urea colorimetric assay kit

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The Urea Colorimetric Assay Kit is a laboratory product designed to quantify urea levels in various samples. It utilizes a colorimetric reaction to measure the concentration of urea present in the sample.

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Lab products found in correlation

Arginase activity assay kit, by Merck Group (2 mentions) Total nitric oxide assay kit, by Beyotime (2 mentions) Williams medium e, by Thermo Fisher Scientific (1 mentions) Quant it protein assay, by Thermo Fisher Scientific (1 mentions) Thermo mutliscanmk3, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Human albumin elisa kit, by Fortis Life Sciences (1 mentions) Mouse igm elisa kit, by Fortis Life Sciences (1 mentions) Mouse igg elisa quantitation set, by Fortis Life Sciences (1 mentions)

8 protocols using urea colorimetric assay kit

1

Urea Production Assay for Hepatocytes

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Urea is produced by hepatocyte as the major end product of nitrogen metabolism and urea production assay is frequently used as an indicator of hepatocyte function. hUCMSCs and i-Heps were cultured with William’s medium E (Gibco) for 24-hour supplemented with or without 0.3 mM NH4Cl (Sigma). Urea concentration in the media was quantified using urea colorimetric assay kit (BioVision, San Francisco, CA) according to the manufacturer’s instructions. Serum-free Williams’ medium E served as the negative control.
Zhou R., Li Z., He C., Li R., Xia H., Li C., Xiao J, & Chen Z.Y. (2014). Human Umbilical Cord Mesenchymal Stem Cells and Derived Hepatocyte-Like Cells Exhibit Similar Therapeutic Effects on an Acute Liver Failure Mouse Model. PLoS ONE, 9(8), e104392.
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2

Nanomembrane-based Analyte Permeation Analysis

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The experimental system that our models attempt to predict the behavior of consists of two channels separated by a 300 micrometer-thick chip supporting a 2-by-0.7 millimeter nanoporous silicon nitride nanomembrane. 1X phosphate buffered saline (pH 7.4) is employed in both the dialysate and the blood channels, and the latter is spiked with a known concentration of an analyte before the experiment begins.
A photograph of the system is shown in Figure 1. The dialysate channel is 2 millimeters wide and 300 micrometers tall, while the blood channel is 1 millimeter wide and has a variable height (100, 300, or 1000 micrometers). The flow rate in the dialysate channel was 9 cubic millimeters per second, and varied in the blood channel depending on the channel height (0.02, 0.06, or 0.2 cubic millimeters per second) to obtain an average velocity of 0.2 millimeters per second.
The results of each experiment were obtained by assaying the fluid at the blood channel outlet for the appropriate analyte. Three analytes and three assays were used: urea (BioVision Urea Colorimetric Assay Kit, catalog #K375–100), cytochrome c (absorbance at 410 nanometers), and bovine serum albumin (ThermoFisher Scientific Quant-iT Protein Assay, catalog #Q33210).
Burgin T., Johnson D., Chung H., Clark A J.r, & McGrath J. (2016). ULTRATHIN SILICON MEMBRANES FOR IMPROVING EXTRACORPOREAL BLOOD THERAPIES. Proceedings of the ... International Conference on Nanochannels, Microchannels and Minichannels. International Conference on Nanochannels, Microchannels and Minichannels, 2016, V001T15A003.
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3

Quantifying Nitric Oxide and Urea Production in Macrophages

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Total NO concentration in culture medium and cells was calculated by measuring the nitrate and nitrite concentrations with a Total Nitric Oxide Assay Kit (Beyotime, China) according to the manufacturer’s instructions. The optical densities at 540 nm were recorded using a Microplate Reader (Thermo MutliscanMK3; Thermo Fisher Scientific, Waltham, MA, USA). The concentration of NO output was calculated from the standard curve. Urea production was determined using a Urea Colorimetric Assay Kit (BioVision). Five million macrophages were harvested and lysed for 30 min in 100 μL of 10 mM Tris–HCl, pH 7.4, containing 0.4% (w/v) Triton X-100. Then, the cells were centrifuged at 13,000 rpm for 10 min. The supernatant was collected for the Arginase Activity Assay kit (Sigma, MAK112). The quantitative data were expressed as the mean ± SD. One-way ANOVA was used to determine the statistical significance.
Chen X.H., Liu S.R., Peng B., Li D., Cheng Z.X., Zhu J.X., Zhang S., Peng Y.M., Li H., Zhang T.T, & Peng X.X. (2017). Exogenous l-Valine Promotes Phagocytosis to Kill Multidrug-Resistant Bacterial Pathogens. Frontiers in Immunology, 8, 207.
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4

Quantifying Insect Urea Excretion

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After allowing bugs to excrete for 24 hrs, they were removed from the assay plate, each well filled with 100 μL of H2O and contents resuspended by pipetting. Solutions were transferred to centrifuge tubes and centrifuged at 10,000 × g at 4 °C for 5 min to remove erythrocyte-derived materials. Urea content was quantified using the Urea Colorimetric Assay Kit (Cat # K375-100, BioVision, Milpitas, CA) following the manufacturers suggested protocol using 20 μL of the excreta solution per 100 μL of final reaction volume. Significant differences in excreted urea between treatments were assessed by T-test.
Tsujimoto H., Sakamoto J.M, & Rasgon J.L. (2017). Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius. Scientific Reports, 7, 3214.
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5

Measuring Hepatic Urea Production in MSCs

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The urea production of MSCs after 3 weeks of hepatic differentiation was investigated. Cytoplasmic protein was extracted to analyze the urea production using a Urea Colorimetric Assay Kit (BioVision).
Wu H.H., Ho J.H, & Lee O.K. (2016). Detection of hepatic maturation by Raman spectroscopy in mesenchymal stromal cells undergoing hepatic differentiation. Stem Cell Research & Therapy, 7, 6.
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6

Nitric Oxide and Urea Production Assays

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NO release in serum or tissues was calculated by examining the nitrate and nitrite concentrations with a Total Nitric Oxide Assay kit (Beyotime, Haimen, China). Optical densities at 540 nm were verified using a microplate reader (Biotek Instruments, Winooski, USA) and NO concentrations were calculated from a standard curve. Urea production in serum was determined using a Urea Colorimetric Assay kit (BioVision, Milpitas, USA). Mouse serum, kidney and liver were collected for the Arginase Activity Assay kit (MAK112, Sigma-Aldrich, San Luis, USA) according to the manufacturer's instructions.
Pang R., Zhou H., Huang Y., Su Y, & Chen X. (2020). Inhibition of Host Arginase Activity Against Staphylococcal Bloodstream Infection by Different Metabolites. Frontiers in Immunology, 11, 1639.
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7

Functional Evaluation of Hepatocyte Cultures

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To test the functionality of the hepatocytes and hepatoma cultures, aliquots of media was collected every 2 days from both static and dynamic perfusion cultures. Albumin content in the medium was measured using the Human Albumin ELISA kit (E88-129, Bethyl Laboratories, Inc. Montgomery, TX, USA) following manufacturer’s instructions. Culture media was diluted 1:2 before assaying.
Urea content was measured using colorimetric Urea assay kit (ab83362, Abcam, Cambridge, UK) following manufacturer’s instructions. Both static and dynamic perfusion culture media was diluted 1:32 before assaying.
Sassi L., Ajayi O., Campinoti S., Natarajan D., McQuitty C., Siena R.R., Mantero S., De Coppi P., Pellegata A.F., Chokshi S, & Urbani L. (2021). A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs. Nanomaterials, 11(2), 275.
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8

Serum Biomarkers in Murine Model

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Serum levels of antibodies to double stranded (ds) DNA (total IgA, IgG, and IgM) (Mouse anti-dsDNA antibodies total Ig ELISA Kit, Alpha Diagnostic, San Antonio, USA), IgM (Mouse IgM ELISA kit, Bethyl Laboratories, Montgomery, USA), IgG (Mouse IgG ELISA quantitation set, Bethyl Laboratories), interleukin-6 (IL-6) (Mouse IL-6 ELISA Kit, Life Technologies), urea (colorimetric Urea Assay Kit, Abcam, Cambridge, United Kingdom), C-terminal type I collagen (CTX-I, ELISA RatLaps kit, Immunodiagostic Systems, Boldon, UK), procollagen type I N propeptide (PINP, Immunodiagostic Systems), and parathyroid hormone (PTH, Elabscience, Houston, USA) were analyzed according to the manufacturers’ instructions.
Nordqvist J., Engdahl C., Scheffler J.M., Gupta P., Gustafsson K.L., Lagerquist M.K., Carlsten H, & Islander U. (2022). A tissue-selective estrogen complex as treatment of osteoporosis in experimental lupus. Lupus, 31(2), 143-154.
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