Cables1 and p21 cDNAs were amplified by PCR and cloned into the Gateway expression vectors (Invitrogen). pLKO.1 Cables1 shRNA1 with target sequence (5’-GCACTTACTTACTACTGGAAA-3’), pLKO.1 Cables1 shRNA2 with target sequence (5’-CCTGGGAGACTTTATGGACTA-3’), pLKO.1 p21 shRNA with target sequence (5’-GTCACTGTCTTGTACCCTTGT-3’) and scrambled shRNA were purchased from OpenBiosystems. Site-directed mutagenesis was performed essentially following the manufacturer’s protocol (Stratagene). Transfections were performed using FuGene HD (Roche).
Plko 1 cables1 shrna1
Manufactured by Thermo Fisher Scientific
The PLKO.1 Cables1 shRNA1 is a lab equipment product designed for use in molecular biology research. It serves as a tool for the stable expression of short hairpin RNA (shRNA) in mammalian cells. The product provides the necessary components for the construction and expression of shRNA, which can be used to study gene function and regulation.
Automatically generated - may contain errors
2 protocols using plko 1 cables1 shrna1
1
Cloning and Silencing of Cables1 and p21
2
Cloning and Silencing of Cables1 and p21
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cables1 and p21 cDNAs were amplified by PCR and cloned into the Gateway expression vectors (Invitrogen). pLKO.1 Cables1 shRNA1 with target sequence (5’-GCACTTACTTACTACTGGAAA-3’), pLKO.1 Cables1 shRNA2 with target sequence (5’-CCTGGGAGACTTTATGGACTA-3’), pLKO.1 p21 shRNA with target sequence (5’-GTCACTGTCTTGTACCCTTGT-3’) and scrambled shRNA were purchased from OpenBiosystems. Site-directed mutagenesis was performed essentially following the manufacturer’s protocol (Stratagene). Transfections were performed using FuGene HD (Roche).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!