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Amd3100

Manufactured by Santa Cruz Biotechnology
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AMD3100 is a small molecule that functions as a CXCR4 antagonist. It is used in research applications.

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Lab products found in correlation

Nigericin, by Merck Group (2 mentions) Wortmannin, by Apexbio (2 mentions) Gallein, by Bio-Techne (2 mentions) Swainsonine, by Merck Group (2 mentions) Rapamycin, by Merck Group (2 mentions) Monensin, by Merck Group (2 mentions) Human sdf 1α, by Thermo Fisher Scientific (2 mentions) Uk14304, by Thermo Fisher Scientific (2 mentions) As 604850, by Apexbio (2 mentions) Gsk2292767, by Apexbio (2 mentions) Tgx 221, by Adooq (2 mentions) Hs 173, by Adooq (2 mentions) Si sdf 1, by GenePharma (2 mentions) Mir 15a 5p mimic, by GenePharma (2 mentions) Mir nc, by GenePharma (2 mentions) Pertussis toxin, by List Biological Laboratories (1 mentions) Erk1 2 antibody, by Cell Signaling Technology (1 mentions) Antibodies against phospho erk1 2, by Santa Cruz Biotechnology (1 mentions) Arf1 antibodies, by Abcam (1 mentions) 12 well insert, by Corning (1 mentions)

8 protocols using amd3100

1

Inhibition of CCR5, CXCR4, and NOX2

Cited 1 time
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Maraviroc (100 nM, Santa Cruz Biotechnology) and AMD3100 (50nM, Santa Cruz Biotechnology) were used as specific antagonists of CCR5 and CXCR4 receptors, respectively. NOX2 was inhibited with TG6-227 kindly provided by Dr. J. D. Lambeth, Emory University [19 (link)]. Pharmacological inhibitors were added to cultures 1 h prior to gp120 exposure and maintained for the duration of the experiment.
Smith L.K., Babcock I.W., Minamide L.S., Shaw A.E., Bamburg J.R, & Kuhn T.B. (2021). Direct interaction of HIV gp120 with neuronal CXCR4 and CCR5 receptors induces cofilin-actin rod pathology via a cellular prion protein- and NOX-dependent mechanism. PLoS ONE, 16(3), e0248309.
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2

Investigating SDF1α-Mediated Cell Migration

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Human SDF1α was purchased from PeproTech; UK14304, rapamycin, brefeldin A, monensin, nigericin, swainsonine, and Exo2 were from Sigma–Aldrich; pertussis toxin was from List Biological Laboratories; gallein was from Tocris Bioscience; wortmannin, AS-604850, and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience; secinH3, GCA, nocodazole, ilimaquinone, AMD3100, control siRNA, and siRNA targeting human ARF1, CRISPR–Cas9 control plasmids, and knockout plasmids targeting human ARF1, and antibodies against phospho-ERK1/2 and β-actin were from Santa Cruz Biotechnology; p230 antibodies were from BD Transduction Laboratories; ARF1 antibodies were purchased from Abcam; ERK1/2 antibodies were from Cell Signaling Technology; 12-well inserts and Matrigel matrix were from Corning. All other materials were obtained as described elsewhere (9 (link), 19 (link), 27 (link)).
Khater M., Bryant C.N, & Wu G. (2021). Gβγ translocation to the Golgi apparatus activates ARF1 to spatiotemporally regulate G protein–coupled receptor signaling to MAPK. The Journal of Biological Chemistry, 296, 100805.
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3

Integrin Beta-6 Monoclonal Antibody Protocol

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The mouse-anti-human monoclonal antibody R6G9 (IgG2a), which is directed against the extracellular domain of human integrin subunit β6, was obtained from Chemicon International (Temecula, CA, U.S.A.). The following monoclonal antibodies were obtained from Abcam (Cambridge, MA, U.S.A.): ab5694 and ab28244, which target the CAF markers α-SMA and FAP, respectively: ab27969, which targets transforming growth factor β (TGF-β); and ab9797, ab9695, ab16828, and ab6672, which target the four cytokines stromal cell-derived factor-1 (SDF-1), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and interleukin 6 (IL-6). EP1186Y and EP1254 monoclonal antibodies, which target matrix metalloproteinase (MMP) 3 (MMP-3) and MMP-9, respectively, were also purchased from Abcam. Reagents for SDS/PAGE and molecular weight markers were obtained from Bio-Rad Laboratories (Hercules, CA, U.S.A.). The C–X–C chemokine receptor type 4 axis (CXCR4) antagonist AMD3100 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.).
Peng C., Zou X., Xia W., Gao H., Li Z., Liu N., Xu Z., Gao C., He Z., Niu W., Fang R., Biswas S., Agrez M., Zhi X, & Niu J. (2018). Integrin αvβ6 plays a bi-directional regulation role between colon cancer cells and cancer-associated fibroblasts. Bioscience Reports, 38(6), BSR20180243.
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4

Inhibition of Signaling Pathways

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Insulin and Metformin were purchased from Merck Life Science (Milan, Italy). The MEK inhibitor trametinib, the Insulin receptor inhibitor OSI-906 and the PI3Kα inhibitor alpelisib were obtained from MedChemExpress (DBA, Milan, Italy). The CXCR4 antagonist AMD3100 was purchased from Santa Cruz Biotechnology (DBA, Milan, Italy). All compounds were dissolved in DMSO, except Insulin, Metformin and AMD3100 that were solubilized in water.
Scordamaglia D., Cirillo F., Talia M., Santolla M.F., Rigiracciolo D.C., Muglia L., Zicarelli A., De Rosis S., Giordano F., Miglietta A.M., De Francesco E.M., Vella V., Belfiore A., Lappano R, & Maggiolini M. (2022). Metformin counteracts stimulatory effects induced by insulin in primary breast cancer cells. Journal of Translational Medicine, 20, 263.
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5

Investigating PI3Kγ Signaling Pathways

Cited 2 times
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Human SDF1α was purchased from PeproTech; UK14304, GW5074, rapamycin, BFA, ilimaquinone, monensin, nigericin, swainsonine, and LY294002 were from Sigma Aldrich; nocodazole, insulin-like growth factor 1, AMD3100, control siRNA (medium GC), siRNAs targeting to human PI3Kγ regulatory subunits p101 and p87, and antibodies against GFP, phospho-ERK1/2, Gγ9, and β-actin and the PI3Kγ subunits p110γ, p101 and p87 were from Santa Cruz Biotechnology; wortmannin, AS-604850, and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience; U0126 and PD98059 were from Calbiochem; EGF, puromycin, and blasticidin S were from Thermo Fisher Scientific; U-73122 and AZD5363 were from MedChemExpress; U-73433, CRT0066101, and Go6976 were from Cayman Chemical; PTX was from List Biological Laboratories; gallein and rauwolscine were from Tocris Bioscience; D-Luciferin was from GoldBio; antibodies against hemagglutinin and ERK1/2 were from Cell Signaling Technology; antibodies against Gγ3 were from Abcam. All other materials were obtained as described (52 (link), 53 (link)).
Khater M., Wei Z., Xu X., Huang W., Lokeshwar B.L., Lambert N.A, & Wu G. (2021). G protein βγ translocation to the Golgi apparatus activates MAPK via p110γ-p101 heterodimers. The Journal of Biological Chemistry, 296, 100325.
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6

Apoptosis Evaluation in Cell Lines

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The αMEM medium, fetal calf serum (FCS) and phosphate buffered saline (PBS) were from Invitrogen. The trypan blue and the anti-LC3 antibody were from Sigma (St Quentin Fallavier, France). The annexin-V-APC was from Biolegend (France). The PIK-III was purchased from Selleck Chemicals (Houston, TX, USA). Cell apoptosis was assessed using an APC-conjugated annexin V labeling detection kit coupled to flow cytometry and BDFACSDIVATM software Becton Dickinson, Le Pont de Claix, France. The murine G-CSF was from Peprotech (Neuilly, France) and AMD3100 from SantaCruz (Heidelberg, Germany). Gilteritinib and SAR-405 were purchased from MedChemTronica (Sollentuna, Sweden).
Dupont M., Huart M., Lauvinerie C., Bidet A., Guitart A.V., Villacreces A., Vigon I., Desplat V., El Habhab A., Pigneux A., Ivanovic Z., Brunet De la Grange P., Dumas P.Y, & Pasquet J.M. (2022). Autophagy Targeting and Hematological Mobilization in FLT3-ITD Acute Myeloid Leukemia Decrease Repopulating Capacity and Relapse by Inducing Apoptosis of Committed Leukemic Cells. Cancers, 14(2), 453.
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7

Modulating SDF-1, XIST, and miR-15a-5p in CAFs

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The construction and packaging of SDF-1 interference vector si-SDF-1 and control si-NC, lncRNA XIST interference vector si-XIST and control Xist NC, miR-15a-5p mimic and miR-NC were carried out by Gene Pharma (Shanghai, China). Lipofectamine 3000 (Thermo Fisher Scienti c) was used for transfection according to the instructions. AMD3100 (Catalog No. CAS 155148-31-5) was purchased from Santa Cruz biotechnology, Inc. (Dallas, TX, USA) and dissolved in dimethyl sulphoxide (DMSO) at 2 µmol/L. The following experiment was carried out 48 hours later.
Cells were assigned into NFs, CAFs, CAFs-si-NC, CAFs-si-SDF-1, CAFs + DMSO, CAFs + AMD3100, CAFs + si-NC, CAFs + si-XIST, CAFs + miR-NC and CAFs + miR-15a-5p groups.
Wang L., Chen W., Li X, & Zhao H. (2022). WITHDRAWN: Stromal Cell-Derived Factor 1 Secreted by Cancer- Associated Fibroblasts Promotes lncRNA Xist through CXCR4 and Facilitates Invasion and Metastasis of Non-Small Cell Lung Cancer. Current cancer drug targets.
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8

Modulating SDF-1, XIST, and miR-15a-5p in CAFs

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The construction and packaging of SDF-1 interference vector si-SDF-1 and control si-NC, lncRNA XIST interference vector si-XIST and control Xist NC, miR-15a-5p mimic and miR-NC were carried out by Gene Pharma (Shanghai, China). Lipofectamine 3000 (Thermo Fisher Scienti c) was used for transfection according to the instructions. AMD3100 (Catalog No. CAS 155148-31-5) was purchased from Santa Cruz biotechnology, Inc. (Dallas, TX, USA) and dissolved in dimethyl sulphoxide (DMSO) at 2 µmol/L. The following experiment was carried out 48 hours later.
Cells were assigned into NFs, CAFs, CAFs-si-NC, CAFs-si-SDF-1, CAFs + DMSO, CAFs + AMD3100, CAFs + si-NC, CAFs + si-XIST, CAFs + miR-NC and CAFs + miR-15a-5p groups.
Wang L., Chen W., Li X., & Zhao H. (2021). Stromal cell-derived factor 1 secreted by cancer-associated fibroblasts promotes lncRNA Xist through CXCR4 and facilitates invasion and metastasis of non-small cell lung cancer.
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