Omnicure series 2000

Manufactured by Excelitas

Sourced in Canada

The OmniCure®Series 2000 is a UV curing system designed for industrial applications. It features a high-intensity, long-life mercury lamp and a compact, modular design. The system is capable of delivering consistent, uniform UV energy across a wide range of substrate materials.

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Lab products found in correlation

Irgacure 2959, by Merck Group (2 mentions) Tmspma, by Merck Group (2 mentions) Pegda, by JenKem Technology (2 mentions) Ar g2 rheometer, by TA Instruments (1 mentions) 24 well plate, by Greiner (1 mentions)

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Exfo Omnicure Series 2000 (2 citations)

14 protocols using omnicure series 2000

Photopolymerization Kinetics of Hybrid Systems

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Photopolymerization kinetics of the
hybrid systems were monitored
using real-time Fourier transform infrared (RT-FTIR) spectroscopy
in attenuated total reflectance (ATR) mode (INVENIO-R FTIR, Bruker).
Hybrid systems were applied to a NaCl pellet with a rod #8 type applicator
with a thickness of 12 μm (BYK). The samples were exposed to
UV radiation for 900 s (Omnicure series 2000, Excelitas) via a fiber
optic light guide emitting between 250 and 500 nm and with an intensity
of 2.5 W/cm². RT-FTIR spectroscopy allows monitoring of
the degree of conversion of each component of the system independently.
The acrylate conversion was determined through the decrease of the
IR band at 1640 cm^–1 corresponding to the acrylate
double bond, and the symmetrical stretching at 790 cm^–1 was chosen for the epoxide ring.^{7 (link)} Percent
conversion was calculated using eq 2, where A₀ is the absorbance
before irradiation and A_t is the absorbance
at time t. In addition to conversion, the maximum rate
of polymerization (R_p^max) was
also obtained via RT-FTIR. R_p^max was taken as the maximum of the first derivative of the conversion
vs. time curves and expressed in %/s.

Calvez I., Szczepanski C.R, & Landry V. (2022). Hybrid Free-Radical/Cationic Phase-Separated UV-Curable System: Impact of Photoinitiator Content and Monomer Fraction on Surface Morphologies and Gloss Appearance. Macromolecules, 55(8), 3129-3139.

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Culturing Cells on Laminin-Coated Surfaces

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Each sample was transferred to a 6 well plate and sterilized in 70% ethanol for 15 minutes, followed by three washes with 1X PBS. Samples were then incubated with 1.5 mL of poly-d-lysine hydrobromide solution (0.1 mg mL⁻¹ in sterile water) at 4°C overnight. After overnight incubation, the poly-d-lysine solution was aspirated, and the samples were washed with sterile water. Meanwhile, 6 mm glass cloning cylinders (Sigma-Aldrich) were sterilized by submersion in 70% ethanol overnight followed by three washes with 1X PBS. These were then air-dried in a sterile environment for approximately 15 minutes prior to their use. Vacuum grease was sterilized by exposure to UV light (3 W cm⁻² for 90 seconds, Omnicure Series 2000 equipped with 8 mm liquid light guide, Excelitas Technologies, Waltham, MA), then applied to one end of the cloning cylinder, without greasing the interior of the cylinder, and then placed directly over the printed structures. The cylinders were then gently pressed down to ensure adhesion to the glass coverslip. Finally, 50 μL of recombinant human laminin 521 solution (50 μg mL⁻¹ in HBSS +/+) were added to each cylinder and the samples were incubated overnight.

Worthington K.S., Do A.V., Smith R., Tucker B.A, & Salem A.K. (2018). Two-Photon Polymerization as a Tool for Studying 3D Printed Topography-Induced Stem Cell Fate. Macromolecular bioscience, 19(2), e1800370.

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Hydrogel Swelling and Mechanical Characterization

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30 μL
of prepolymer solution with both 80 DOF and 50 DOF (5 and 10%) was
pipetted onto a 10 cm Petri dish between two spacers with a height
of 0.45 mm and was covered with a sterile glass slide. The prepolymer
solution was placed under a 450 mW ultraviolet (UV)-light (OmniCure
Series 2000, Excelitas) for 50 s. The hydrogel was removed from the
glass slide and washed with PBS. Empty hydrogels were incubated in
PBS at 37 °C for 24 h before mechanical testing and hydrogel
swelling analysis.
Swollen GelMa hydrogels (3 per experiment
for 5 different experiments) were weighed (ww) and subsequently dried
by lyophilization. After that, dried weight (wd) of GelMa hydrogels
was obtained and the mass-swelling ratio (q) was
calculated as q = ww/wd.

Meijer E.M., Giles R., van Dijk C.G., Maringanti R., Wissing T.B., Appels Y., Chrifi I., Crielaard H., Verhaar M.C., Smits A.I, & Cheng C. (2023). Effect of Mechanical Stimuli on the Phenotypic Plasticity of Induced Pluripotent Stem-Cell-Derived Vascular Smooth Muscle Cells in a 3D Hydrogel. ACS Applied Bio Materials, 6(12), 5716-5729.

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Generating BSA Gradient in GelMA Hydrogel

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To generate a gradient of BSA inside GelMA hydrogel, 120 μl of GelMA prehydrogel solution without BSA was dropped into a 24-well plate and photocrosslinked to create the base GelMA hydrogel. Then, a set of GelMA prehydrogel solutions with BSA (80 μl each) were pipetted onto the base and photocrosslinked sequentially by UV light (OmniCure, series 2000, Excelitas Technologies), from low to high concentrations of BSA. Gradients of BSA inside PEGDA hydrogel were also generated in the same manner.

Ko H., Suthiwanich K., Mary H., Zanganeh S., Hu S.K., Ahadian S., Yang Y., Choi G., Fetah K., Niu Y., Mao J.J, & Khademhosseini A. (2019). A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels. Biofabrication, 11(2), 025014.

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Rheological Characterization of PF Hydrogels

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Rheological characterization was done using AR-G2 rheometer (TA Instrument, United States) as described elsewhere (Gonen-Wadmany et al., 2011 (link); Mironi-Harpaz et al., 2012 (link)). A PF hydrogel precursor solution of 200 μl was loaded onto a 20 mm diameter parallel-plate geometry. The PF solution was equilibrated for 1 min before being exposed to 365 nm UV light with the intensity of 5 mW cm^-1 from an Omnicure Series 2000 light source (Excelitas Technologies Corp., Waltham, MA, United States). Shear modulus data from dynamic time-sweeps were collected during the photopolymerization of the PF solution upon activation with the UV light source. At the end of the crosslinking process, the shear loss modulus (G”) and shear storage modulus (G’) were collected.

Rufaihah A.J., Cheyyatraivendran S., Mazlan M.D., Lim K., Chong M.S., Mattar C.N., Chan J.K., Kofidis T, & Seliktar D. (2018). The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells. Frontiers in Physiology, 9, 1555.

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Encapsulation of Vascular Cells in GelMA Hydrogels

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The ODMCs/VSMCs were added to the GelMa-PI solution to achieve a
cell density of 75.000 cells per 30 μL. The cell containing
GelMa solution (30 μL) was pipetted on a 10 cm Petri dish between
two spacers covered with a sterile microscope slide and placed under
a 450 mW UV light (OmniCure Series 2000, Excelitas) for 50 s. The
hydrogels were subjected to serum starvation (0.5% DMEM) for 24 h,
after which 10% DMEM was added. N = 6 per condition.

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Hydrogel Microwell Array Fabrication

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UV‐photocrosslinkable PEGDA (Jenkem Technology) of molecular weight 3500 Da were mixed with photoinitiator Irgacure 2959, HHEMP (Sigma‐Aldrich) and diluted with 1xPBS to form a prepolymer solution comprising of the photoinitiator. The patterned PDMS stamp was placed on an evenly distributed film of prepolymer solution on a TMSPMA (Sigma‐Aldrich)‐treated cover slip, with two coverslips placed on both sides as spacers. Photopolymerization was achieved by irradiating the set‐up with UV light of 320‐500 nm and at an intensity of 4.96 W/cm² for 30 seconds using the OmniCure^®Series 2000 curing station (Lumen Dynamics) as previously optimized. After photopolymerization, the PDMS stamp was peeled from the fabricated hydrogel microwell arrays, which were submerged in 70% ethanol for 2 hours to remove excess prepolymer solution. Hydrogel microwell arrays were subsequently washed thrice with PBS and stored in sterile PBS under aseptic conditions prior to cell seeding.

Tan J.J., Common J.E., Wu C., Ho P.C, & Kang L. (2019). Keratinocytes maintain compartmentalization between dermal papilla and fibroblasts in 3D heterotypic tri‐cultures. Cell Proliferation, 52(5), e12668.

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Hydrogel Stability Evaluation

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Hybrid-hydrogels and fully synthetic hydrogels (17 wt% poly(ethylene glycol)-methacrylate (PEGMA; 10 kg/mol) crosslinked with 100% DTT) were fabricated, swollen in 2.2 mM LAP, stiffened by exposure to light (365 nm light, 10 mW/cm²) for 5 min (OmniCure Series 2000; Lumen Dynamics), and two assays were performed on each condition every 10 days for up to 60 days to examine the hydrolytic stability. Rheology was completed as described above on each sample before the sample was placed in DI water, lyophilized, and weighed to record the dry polymer mass.

Petrou C.L., D’Ovidio T.J., Bölükbas D.A., Tas S., Brown R.D., Allawzi A., Lindstedt S., Nozik-Grayck E., Stenmark K.R., Wagner D.E, & Magin C.M. (2020). Clickable decellularized extracellular matrix as a new tool for building hybrid-hydrogels to model chronic fibrotic diseases in vitro. Journal of materials chemistry. B, 8(31), 6814-6826.

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Fabrication of Hydrogel Microwell Arrays

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UV-photocrosslinkable GelMA synthesized earlier or PEGDA (Jenkem Technology, USA) of molecular weight 3500 Da was mixed with photoinitiator Irgacure 2959, HHEMP (Sigma-Aldrich, USA) and diluted with 1× PBS to form a prepolymer solution containing the photoinitiator. The patterned PDMS stamp was placed on an evenly distributed film of prepolymer solution on a TMSPMA (Sigma-Aldrich, New York, NY, USA)-treated cover slip, with 2 coverslips set on both sides as spacers. Photopolymerization was attained by irradiating the set-up with UV light of 320–500 nm and at an intensity of 4.96 W/cm² for 30 s using the OmniCure®Series 2000 curing station (Lumen Dynamics, Canada) as previously optimized. After photopolymerization, the PDMS stamp was removed from the fabricated hydrogel microwell arrays, which were submerged in 70% ethanol for 2 h to remove excess prepolymer solution. Hydrogel microwell arrays were then washed thrice with PBS and stored in sterile PBS under aseptic conditions prior to cell seeding.

Tan J.J., Nguyen D.V., Common J.E., Wu C., Ho P.C, & Kang L. (2021). Investigating PEGDA and GelMA Microgel Models for Sustained 3D Heterotypic Dermal Papilla and Keratinocyte Co-Cultures. International Journal of Molecular Sciences, 22(4), 2143.

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Evaluating Cell Adhesion on Hydrogel Coatings

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Different wells from a 24 well plate (Greiner bio-one, Austria) were coated with 300 µL of 5% PEGDA or 5% GelMA and cured under UV light of 320–500 nm and at an intensity of 4.96 W/cm² for 30 s using the OmniCure®Series 2000 curing station (Lumen Dynamics, Canada). Wells which were not coated with either PEGDA or GelMA were used as a control. These are wells from normal, non-treated wells in the 24 well plate. DP cells were seeded at 50,000 cells into the respective wells and incubated for 24 h. Non-adherent cells were washed away with PBS at 24 h after cell seeding. Attached cells were detached by trypsin/EDTA and counted by use of a hemocytometer in 20 µL per sample.

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