Fcεri mar 1

Manufactured by BioLegend

FcεRI (Mar-1) is a monoclonal antibody that recognizes the high affinity receptor for IgE, FcεRI, on human basophils and mast cells. It can be used for the identification and enumeration of these cell types by flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Ionomycin, by Thermo Fisher Scientific (2 mentions) Aria 2 cytometer, by BD (2 mentions) Facsdiva 6, by BD (2 mentions) Phorbol 12 myristate 13 acetate, by Thermo Fisher Scientific (2 mentions) F4 80 pe cy7 bm8, by BioLegend (2 mentions) Live dead yellow, by Thermo Fisher Scientific (2 mentions) Cd11b percp cy5.5 m1 70, by BioLegend (2 mentions) I a 1 e af488, by BioLegend (2 mentions) Gr 1 pacific orange rb6 8c5, by Thermo Fisher Scientific (2 mentions) Cd11c apc cy7 hl3, by BD (2 mentions) Cd117 pe cy5 2b8, by BioLegend (2 mentions) Ly6 c pacific blue hk1, by BioLegend (2 mentions) Ly6 g af647 1a8, by BioLegend (2 mentions) Il 17a pacific blue tc11 18h10, by BioLegend (2 mentions) Cytofix cytoperm, by BD (2 mentions) Golgistop, by BD (2 mentions) Lsrii cytometer, by BD (2 mentions) Cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Cd31 390, by Thermo Fisher Scientific (1 mentions) Cd34 ram34, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FITC-conjugated anti-FcεRI (MAR-1, Biotinylated anti-FcεRI (clone MAR-1, MAR-1 FcεRI

3 protocols using fcεri mar 1

Multicolor Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions were stained with Live/Dead Yellow (Life Technologies, Grand Island, NY), CD11b-PerCp/Cy5.5 (M1/70, Biolegend, San Diego, CA), I-A/I-E-AF488 (M5/114.15.2, Biolegend), Gr-1-Pacific Orange (RB6-8C5, Life Technologies), F4/80-PE-Cy7 (BM8, Biolegend), CD11c-APC-Cy7 (HL3, BD Biosciences, San Jose, CA), CD117-PE-Cy5 (2B8, Biolegend) and FcεRI (Mar-1, Biolegend). Ly6-C-Pacific Blue (HK1.4, Biolegend) and Ly6-G-AF647 (1A8, Biolegend) were used in adoptive transfer experiments. For intracellular cytokine staining, cells were stimulated for 4.5 h with 30 nM phorbol 12-myristate 13-acetate and 1 μM ionomycin (both eBioscience) in presence of Golgistop (BD). Cells were subsequently stained against cell surface markers (CD4-PerCp/Cy5.5, GK1.5; CD3-AF700, 17A2; CD8-PE/CF594, 53-6.7; all Biolegend) followed by fixation/permeabilization (Cytofix/Cytoperm, BD) and staining using IL-17A-Pacific Blue (TC11-18H10.1, Biolegend). Flow cytometry was performed using a LSRII cytometer (BD) and data were analyzed with FACSDi-Va 6.1.3 software (BD). For cell sorting (FACS) experiments, myeloid populations were sorted using an AriaII cytometer (BD). MO-MDSCs were sorted as CD45⁺CD3^-CD11b^hiGr-1^lowF4/80^-/lowMHC-II^-/low, PMN-MDSCs as CD45⁺CD3^-CD11b^hiGr-1^hiF4/80^-MHC-II^-, and MΦ as CD45⁺CD3^-CD11b^hiGr-1^-/lowF4/80^hiMHC-II⁺ cells.

Orberg E.T., Fan H., Tam A.J., Dejea C.M., Destefano-Shields C.E., Wu S., Chung L., Finard B.B., Wu X., Fathi P., Ganguly S., Fu J., Pardoll D.M., Sears C.L, & Housseau F. (2016). The Myeloid Immune Signature of Enterotoxigenic Bacteroides Fragilis-Induced Murine Colon Tumorigenesis. Mucosal immunology, 10(2), 421-433.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against the following antigens were used: CD11b (M1/70; eBioscience), CD49b (DX5; eBioscience), Siglec-F (E50-2440; BD), Ly6G/C (RB6-8C5; BD), FcεRI (Mar-1; BioLegend), CD45 (30-F11; BioLegend), CD34 (RAM34; eBioscience), ESAM-1 (1G8; BioLegend), CD31 (390; eBioscience), c-kit (2B7; BioLegend), CD106 (429; eBioscience), IL-4Rα (mIL4-M1; BD), and CD213a1 (13MOKA; eBioscience). Key cell populations were defined as follows: (a) eosinophils, FSC^loSSC^hiCD11b⁺Siglec-F⁺ or FSC^loSSC^hiSiglec-F⁺4get⁺; (b) basophils, FSC^loSSC^loCD49b⁺FcεRI⁺Gr-1⁻CD4⁻CD8⁻CD19⁻γδ⁻Siglec-F⁻ or FSC^loSSC^loBasoph8⁺CD49b⁺; (c) mast cells, 4get⁺ckit⁺ or FSC^hiSSC^hic-kit⁺CD11a⁻CD44⁺; (d) endothelial cells, CD45⁻CD34⁺ESAM-1⁺; and (e) PMNs/monocytes, Gr-1⁺CD11b⁺. Flow cytometry data acquisition was performed on an LSRII (BD), using FlowJo to analyze the data.

Cheng L.E., Sullivan B.M., Retana L.E., Allen C.D., Liang H.E, & Locksley R.M. (2015). IgE-activated basophils regulate eosinophil tissue entry by modulating endothelial function. The Journal of Experimental Medicine, 212(4), 513-524.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!