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Ammonium bicarbonate nh4hco3

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Ammonium bicarbonate (NH4HCO3) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. Ammonium bicarbonate serves as a source of ammonium ions and bicarbonate ions, which can be used in various chemical reactions and analyses.

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Lab products found in correlation

Iodoacetamide, by Sinopharm (1 mentions) Dithiothreitol (dtt), by Sinopharm (1 mentions) Trypsin, by Promega (1 mentions) Formic acid, by Sinopharm (1 mentions) Trifluoroacetic acid, by Sinopharm (1 mentions) Iodoacetamide iaa, by Merck Group (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Acetic acid, by Sinopharm (1 mentions) Sequencing grade modified trypsin, by Promega (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions)

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NH4HCO3 (6 citations) Ammonium bicarbonate (5 citations)

3 protocols using ammonium bicarbonate nh4hco3

1

Protein Purification and Analysis Protocol

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Trypsin was purchased from Promega Corporation. Dithiothreitol, formic acid, iodoacetamide, ammonium bicarbonate (NH4HCO3) and trifluoroacetic acid were obtained from Sinopharm Chemical Reagent Co., Ltd.. High-performance liquid chromatography (HPLC)-grade acetonitrile (CAN) was acquired from Thermo Fisher Scientific, Inc. Synthetic indexed retention time (SiRT) peptides were obtained from Biognosys AG. The other chemicals were purchased from Sigma-Aldrich (Merck KGaA), unless otherwise indicated.
Jiang L., Rong A., Wei R., Diao J., Ding H, & Wang W. (2020). Tear proteomics of orbital decompression for disfiguring exophthalmos in inactive thyroid-associated ophthalmopathy. Experimental and Therapeutic Medicine, 20(6), 253.
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2

Lactoferrin Purification and Proteomic Analysis

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Ammonium bicarbonate (NH4HCO3), calcium chloride (CaCl2), sodium chloride (NaCl), sodium hydroxide (NaOH), disodium hydrogen phosphate (Na2HPO4), and acetic acid, which were of analytical grade, were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Lactoferrin from bovine milk (>85%), Tris (2-carboxyethyl) phosphine hydrochloride (TCEP), and iodoacetamide (IAA) were obtained from Sigma–Aldrich (St. Louis, MO, USA). Sequencing-grade modified trypsin was from Promega Corporation (Madison, WI, USA). Methanol, acetonitrile, and formic acid of HPLC grade were purchased from Fisher Chemicals (Fair Lawn, NJ, USA). Ultrapure water was obtained from a Milli-Q gradient water system (Millipore, Bedford, MA, USA). HiTrapTM Heparin HP was obtained from GE Healthcare Bio-Science AB (Uppsala, Sweden). Ultracel®-50K centrifugal filters were obtained from Merck Millipore Ltd. (Tullagreen, Carrigtwohill, Ireland).
Li X., Li Z., Xu E., Chen L., Feng H., Chen L., Deng L, & Guo D. (2019). Determination of Lactoferrin in Camel Milk by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Using an Isotope-Labeled Winged Peptide as Internal Standard. Molecules, 24(22), 4199.
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3

Synthesis and Characterization of WS2 Nanoparticles

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Tungsten chloride (WCl6) and oxalic
acid (OA) were purchased from Adamas Reagent Co., Ltd. (Shanghai,
China). LA-PEG (Mw = 5000) was obtained
from Ponsure Biotech, Inc. (Shanghai, China). α-Lipoic acid
and dopamine hydrochloride were acquired from Alfa Aesar chemical
company (China). Cy5.5-PEG-SH (Mw = 2000)
was provided by Shanghai ToYongBio Tech.Inc. (China). Ferric chloride·6H2O (FeCl3·6H2O) and ammonium bicarbonate
(NH4HCO3) were purchased from Sinopharm Group
Co., Ltd. Roswell Park Memorial Institute (RPMI) 1640 medium, fetal
bovine serum (FBS), penicillin–streptomycin, and trypsin were
obtained from BI Corp. Calcein-AM and propidium iodide were obtained
from 4A Biotech Co., Ltd. (Beijing, China). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium
bromide (MTT) was purchased from Thermo Fisher (China). Bulk tungsten
disulfide (WS2, 99.9%, 2 μm) was supplied by Aladdin
Reagent (Shanghai, China). TNF-α and IL-6 ELISA kits were purchased
from 4A Biotech Co., Ltd. (Beijing, China). Carbon monoxide assay
kit (A101-2) was obtained from Nanjing Jiancheng Bioengineering Institute
(China). All other reagents and solvents were used without further
purification.
Wang S.B., Zhang C., Ye J.J., Zou M.Z., Liu C.J, & Zhang X.Z. (2020). Near-Infrared Light Responsive Nanoreactor for Simultaneous Tumor Photothermal Therapy and Carbon Monoxide-Mediated Anti-Inflammation. ACS Central Science, 6(4), 555-565.
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