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Fitc conjugated donkey anti goat antibody

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The FITC-conjugated donkey anti-goat antibody is a secondary antibody that binds to goat primary antibodies. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and visualization of the target antigen in various immunoassay techniques.

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Lab products found in correlation

Confocal laser scanning microscope, by Zeiss (2 mentions) Cryomatrix, by Thermo Fisher Scientific (2 mentions) Goat anti pigr igg antibody, by R&D Systems (2 mentions) Laminin antibody, by Abcam (1 mentions) Cd31 antibody, by Santa Cruz Biotechnology (1 mentions) Nestin antibody, by Merck Group (1 mentions) Cy3 conjugated goat anti rabbit antibody, by Jackson ImmunoResearch (1 mentions) Prolong gold and dapi, by Thermo Fisher Scientific (1 mentions) Cy5 conjugated affinipure donkey anti mouse antibody, by Jackson ImmunoResearch (1 mentions) Fluoview fv10i, by Olympus (1 mentions)

Spelling variants of this product

FITC conjugated donkey anti‐goat secondary antibody (3 citations) FITC-conjugated donkey anti-goat (2 citations) FITC)-conjugated donkey anti-goat IgG antibody (2 citations)

4 protocols using fitc conjugated donkey anti goat antibody

1

Immunofluorescence Analysis of Primary Hepatocytes

Cited 1 time
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Primary hepatocytes were fixed in 4% paraformaldehyde and made into smear. Immunofluorescence was performed as previous described [9 (link)]. Albumin antibody (1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CD31 antibody (1:50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Laminin antibody (1:100, Abcam, Cambridge, UK), and Nestin antibody (1:50, Chemicon, Billerica, MA, USA) were used. FITC-conjugated donkey anti-goat antibody or Cy3-conjugated goat anti-rabbit antibody was used as secondary antibodies (1:100, Jackson Immuno-Research, West Grove, PA). At last, nuclei were stained with DAPI.
Chang N., Tian L., Ji X., Zhou X., Hou L., Zhao X., Yang Y., Yang L, & Li L. (2019). Single-Cell Transcriptomes Reveal Characteristic Features of Mouse Hepatocytes with Liver Cholestatic Injury. Cells, 8(9), 1069.
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2

Immunohistochemical Detection of pIgR

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For pIgR staining, small intestinal tissue was cut longitudinally and rolled over a ‘Swiss roll’, which was then fixed in 4% formaldehyde in PBS for 1 h at 25 °C. Fixed tissues were dehydrated by gradually soaking in sucrose (10 % ~ 30 %) and then embedded in cryomatrix (Thermo scientific). The frozen sections were sequentially reacted with goat anti-pIgR IgG Antibody (R&D Systems) at 4 °C overnight, and then incubated with FITC-conjugated donkey anti-goat antibody (Jackson ImmunoResearch) at room temperature for 2 h and viewed under a confocal scanning laser microscope (Zeiss). The pIgR+ dots were counted on the intestinal villi that were randomly selected in 5 regions per slide. Data shown are the mean values ±s.e.m. of individual mice from 2 independent experiments with 3 mice per experiment.
Jie Z., Yang J.Y., Gu M., Wang H., Xie X., Li Y., Liu T., Zhu L., Shi J., Zhang L., Zhou X., Joo D., Brightbill H.D., Cong Y., Lin D., Cheng X, & Sun S.C. (2018). NIK signaling axis regulates dendritic cell function in intestinal immunity and homeostasis. Nature immunology, 19(11), 1224-1235.
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3

Immunohistochemical Detection of pIgR

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For pIgR staining, small intestinal tissue was cut longitudinally and rolled over a ‘Swiss roll’, which was then fixed in 4% formaldehyde in PBS for 1 h at 25 °C. Fixed tissues were dehydrated by gradually soaking in sucrose (10 % ~ 30 %) and then embedded in cryomatrix (Thermo scientific). The frozen sections were sequentially reacted with goat anti-pIgR IgG Antibody (R&D Systems) at 4 °C overnight, and then incubated with FITC-conjugated donkey anti-goat antibody (Jackson ImmunoResearch) at room temperature for 2 h and viewed under a confocal scanning laser microscope (Zeiss). The pIgR+ dots were counted on the intestinal villi that were randomly selected in 5 regions per slide. Data shown are the mean values ±s.e.m. of individual mice from 2 independent experiments with 3 mice per experiment.
Jie Z., Yang J.Y., Gu M., Wang H., Xie X., Li Y., Liu T., Zhu L., Shi J., Zhang L., Zhou X., Joo D., Brightbill H.D., Cong Y., Lin D., Cheng X, & Sun S.C. (2018). NIK signaling axis regulates dendritic cell function in intestinal immunity and homeostasis. Nature immunology, 19(11), 1224-1235.
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4

Visualizing CD137 Expression in EBV Cells

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The expression of CD137 protein on EBV-infected cells was examined by immune-fluorescent staining. Cells were fixed on slides by immersing in 4% paraformaldehyde for 10 min, followed by washing three times in PBS and incubation with mouse monoclonal anti-CD137, goat polyclonal anti-EBNA1) antibodies (Abcam, Cambridge, MA, USA), Cy5-conjugated Affinipure donkey anti-mouse antibody, and FITC-conjugated donkey anti-goat antibody (Jackson ImmunoResearch Laboratories, Inc. PA, USA). Nuclei were counterstained with ProLong Gold and DAPI (Invitrogen, Carlsbad, CA, USA), and the cells were analyzed by confocal microscopy (Fluoview FV10i, Olympus).
Yoshimori M., Imadome K.I., Komatsu H., Wang L., Saitoh Y., Yamaoka S., Fukuda T., Kurata M., Koyama T., Shimizu N., Fujiwara S., Miura O, & Arai A. (2014). CD137 Expression Is Induced by Epstein-Barr Virus Infection through LMP1 in T or NK Cells and Mediates Survival Promoting Signals. PLoS ONE, 9(11), e112564.
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