Sxz16 microscope

The holotype of Conobregmabradpitti sp. n. is deposited in the Hymenoptera Institute Collection, Department of Entomology, University of Kentucky, Lexington, Kentucky. It was imaged using an Olympus SXZ16 microscope with automated multiple image capture at preset focal levels using an Olympus DP72 camera, and image combination using the Cell^D image processing system. The specimen was card-mounted and rather fragile but we successfully remounted it to enable more features to be seen.
The holotype of Facitorusnasseri sp. n. is deposited in the Department of Zoology, University of Calicut, Kerala, India. It was imaged using an Leica M205A stereomicroPageBreakscope with automated multiple image capture at preset focal levels using an Leica DMC 2900 camera, and image combination using the Leica Application Suite image processing system v4.7. All images were edited using Photoshop CS6 (Version 6.1) (Adobe Inc.).
Terminology follows van Achterberg (1988) except for wing venation nomenclature which follows Sharkey and Wharton (1997) ; see also Figure 2.2 in Quicke (2015) for comparison of wing venation naming systems.

Starvation plates of the evolved populations were imaged after 5 days of starvation for representative pictures (Figs. 1a, 3a and Supp. Fig. 3b) with a Zeiss STEMI 2000 microscope and captured with a Nikon Coolpix S10 camera. Images for quantitative morphological analysis were acquired using an Olympus SXZ16 microscope mounting an Olympus DP80 camera system. The image-acquisition parameters were kept identical in all cases (exposure time = 9.9 ms, lens = Olympus 0.5xPF, zoom = 1.25x, ISO = 200, illumination = BF built-in system). Images were processed and analyzed following the protocol described in ref. ¹¹ . Fruiting body morphology was characterized by measuring the following traits. Fruiting body number: total number of fruiting bodies on a single developmental plate; Density: gray-value intensity of pixels per fruiting body; Density heterogeneity: standard deviation of within fruiting body pixel-gray values (fruiting body density); Fruiting body area: plate-surface area occupied per fruiting body, expressed in total pixel number. For more details about each trait, see ref. ¹¹ . Median values of Density, Density heterogeneity, and Area per plate were used for further analysis. Image acquisition and trait quantification of the evolved populations were always run in parallel with Anc during each biological replicate.