Cfi plan apo lambda 20x

In order to investigate the ultrafast dynamics of compound 1in cellulo, laser scanning microscopy and pump-probe spectroscopy were combined in a transient absorption microscope45 . The technological specifications of this setup can be found in ref. 44 and is schematically shown in Supplementary Fig. 6. In the transient-absorption microscope the samples are scanned in a raster pattern by means of a galvanometer-based scanner system (Cambridge Technologies) that together with the focusing objective (Nikon, CFI Plan Apo Lambda 20X, NA = 0.75, WD = 1 mm) provide diffraction limited optical resolution of the microscope. With this system it is possible to obtain linear absorption as well as transient absorption images and information on the local excited state dynamics by recording pump-probe kinetics in a fixed position of the sample with a temporal resolution of about 1 ps. The samples can be moved along the perpendicular plane of the laser beams with 10 nm precision using a scanning stage (SCAN 75 × 50, Märzhäuser).
Additionally the cellular uptake and cellular distribution of compound 1 in the cells was assessed by confocal laser scanning (using a Carl Zeiss, LSM 510 Meta) and structured illumination microscopy (using a Carl Zeiss, ELYRA-S.1).

WISH and immunohistochemistry were performed as previously described (Nakamura et al., 2006 (link)). For Fig. 3B and C, the expression signals were observed using a BZ-X710 All-in-One Fluorescence Microscope (Keyence) with a 20X objective lens (CFI PlanApo Lambda 20X NA:0.75, Nikon). For Fig. 4F–I, the images of gonad were observed in three parts (anterior, middle and posterior) using a FV1000 confocal microscopy (Olympus) with 60X objective lens (UPLSAPO 60XO NA:1.35, Olympus). The three parts of gonadal images were manually tiled using a software of Illustrator (Adobe CS6) to generate an image of whole view on the gonad.