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Cd4 alexa fluor 647

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The CD4 Alexa Fluor 647 is a fluorescently labeled antibody product that binds to the CD4 cell surface receptor. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cells in biological samples.

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Lab products found in correlation

Fix perm buffer, by BioLegend (1 mentions) Pe foxp3 antibody, by BioLegend (1 mentions) Cd8 percp cy5, by BioLegend (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd8 fitc, by Thermo Fisher Scientific (1 mentions) Cd3 apc, by Thermo Fisher Scientific (1 mentions) Cd8a apc, by Thermo Fisher Scientific (1 mentions) Anti ifn γ pe, by Thermo Fisher Scientific (1 mentions) Rat igg2b κ apc, by BioLegend (1 mentions) Cd274 pd l1 apc, by BioLegend (1 mentions) Cyan adp, by Beckman Coulter (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Fortessa, by BD (1 mentions) Cd206 alexa fluor 647, by BioLegend (1 mentions) Cd19 fitc, by BioLegend (1 mentions) F4 80 alexa fluor 488, by BioLegend (1 mentions) Be0089, by BioXCell (1 mentions) Nk 1.1 alexafluor 647, by BioLegend (1 mentions) Be0146, by BioXCell (1 mentions)

Close spelling variants of this product

Anti-CD4 Alexa Fluor 647 (GK1.5; Alexa Fluor® 647 anti-mouse CD4 CD4-Alexa-647 Alexa Fluor 647 Alexa 647

3 protocols using cd4 alexa fluor 647

1

Fluorescent Immunophenotyping of Lymphoid Cells

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Cell suspensions from cervical lymph nodes, spleen and brain were incubated with CD4 Alexa Fluor 647, CD8 PerCP/Cy5.5, CD25 Alexa Fluor 488 and CD69 Alexa Fluor 488 (Biolegend) for 30 min, subsequently fixed and permeabilized by eBioscience Fix/Perm buffer for 30 min and then incubated with FoxP3-PE antibody (Biolegend) for 1 h. Acquisition was performed on an LSRII flow cytometer (BD) and data analysis was done using FlowJo software (FLOWJO, LLC). In all FACS measurements, compensation controls have been employed using single stained samples of CD4 Alexa Fluor 647 (APC channel), CD8 PerCP/Cy5.5 (PerCP Channel), CD4 PE (PE channel) or CD4 Alexa Fluor 488 (FITC channel).
Badr M., McFleder R.L., Wu J., Knorr S., Koprich J.B., Hünig T., Brotchie J.M., Volkmann J., Lutz M.B, & Ip C.W. (2022). Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson’s disease mice. Journal of Neuroinflammation, 19, 319.
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2

Multiparametric flow cytometry for immune cell profiling

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Flow cytometry was used to determine protein levels on living cells. The following extra-cellular antibodies were used: CD3-APC (clone: 17A2), CD8-FITC (clone: 53-6.7), Rat IgG1, κ-PE isotype control (clone: eBRG1), from eBioscience; CD8a-APC (clone: 53-6.7), PD-1 (CD279)-PE_Cy7 (clone: 29F.1a12), PD-L1 (CD274)-APC (clone: 10F.9G2), CD4-Alexafluor647 (clone: GK1.5), Rat IgG2a, κ-FITC (clone: RTK2758), Rat IgG2b-Alexafluor 647(clone: RTK4530), Rat IgG2b, κ-APC (clone: RTK4530), κ-FITC (clone:RTK2758), from Biolegend. For intracellular protein staining we used anti-IFN-γ-Alexafluor 647 (clone: XMG1.2), anti-IFN-γ-PE (clone: XMG1.2) from eBioscience. Cell viability was monitored with either propidium iodide, from Sigma-Aldrich, Fixable Viability Dye -eFluor780, from eBioscience, or Aquablue Live/Dead Stain from ThermoFisher Scientific; depending on the experiment requirements. Data were acquired on a Fortessa (BD Biosciences) or a CyAn ADP (Beckman Coulter) and analyzed using FlowJo v10.
Mastroianni J., Stickel N., Andrlova H., Hanke K., Melchinger W., Duquesne S., Schmidt D., Falk M., Andrieux G., Pfeifer D., Dierbach H., Schmitt-Graeff A., Meiss F., Boerries M, & Zeiser R. (2018). miR-146a controls immune response in the melanoma microenvironment. Cancer research, 79(1), 183-195.
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3

Characterization of Immune Cell Populations

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Therapeutic anti-PD-1 (BE0146) and isotype control antibody (BE0089) were produced by BioXcell. Antibodies used for flow cytometry were purchased from eBioscience (Anti-mouse CD3ε FITC, CD11b eFluor 660) and Biolegend (Anti-mouse CD8a Alexa Fluor® 647, NK 1.1 Alexa Fluor® 647, Ly-6G/Ly-6C (Gr-1) Alexa Fluor® 488, CD19 FITC, CD45R/B220 Alexa Fluor® 647, F4/80 Alexa Fluor® 488, CD206 Alexa Fluor® 647, FOXP3 Alexa Fluor® 488, CD4 Alexa Fluor® 647, CD11c Alexa Fluor® 488, I-A/I-E Alexa Fluor® 647, CD16/32).
Hu H.J., Liang X., Li H.L., Wang H.Y., Gu J.F., Sun L.Y., Xiao J., Hu J.Q., Ni A.M, & Liu X.Y. (2021). Enhanced anti-melanoma efficacy through a combination of the armed oncolytic adenovirus ZD55-IL-24 and immune checkpoint blockade in B16-bearing immunocompetent mouse model. Cancer Immunology, Immunotherapy, 70(12), 3541-3555.
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