Isrib

Thapsigargin (Sigma, T9033), Tunicamycin (Sigma, T7765), Maprotiline (Sigma, M9651), Amoxapine (MedChemExpress, HY-B0991), Desloratadine (MedChemExpress, HY-B0539), Desipramine (Sigma, D3900), Trifluoperazine (Sigma, T8516), Clomipramine (Sigma, C7291), Amitriptyline (Sigma, A8404), Quetiapine (Sigma, Q3638), Olanzapine (MedChemExpress, HY-14541), Doxepin (MedChemExpress, B078), Loxapine (Sigma, L106), NorQuetiapine (Sigma, 07849), dimethyl sulfoxide (DMSO) (Sigma, D8418), 2-APB (MedChemExpress, HYW009724), U-73122 (MedChemExpress, HY13419), Go 6983 (MedChemExpress, HY13689), GSK2606414 (Sigma, 516535), ISRIB (MedChemExpress, HY-12495).

Phenformin (Sigma, Santa Fe, NM, USA) and metformin (Sigma) stock solutions were prepared with phosphate buffered saline (PBS) for in vitro and in vivo studies.^{29 (link)} Chloroquine (CQ), MHY1485, ISRIB, and GSK2606414 were obtained from MedChemExpress (Monmouth Junction, NJ, USA) and were diluted with DMSO for in vitro studies.

AML-12 cells are preserved in the School of Pharmacy, Anhui Medical University. Anti-NUP85 was bought from Santa Cruz Biotechnology (CA, USA). Anti-CCR2 was bought from Abcam (Cambridge, UK). Anti-phosphorylated protein kinase B (p-AKT), anti-AKT, anti-phosphorylated phosphatidylinositol-3 kinase (p-PI3K) and anti-PI3K were bought from Immunoway (Suzhou, China). Anti-β-actin, anti-acyl-CoAoxidase-1 (ACOX-1) and anti-interleukin-1beta (IL-1β) were got from Proteintech (Wuhan, China). Anti-sterol regulatory element binding protein-1 (SREBP-1C) was purchased from Affinity Biotechnology (Liyang, China). Anti-peroxisome proliferator-activated receptor alpha (PPAR-α), anti-interleukin-6 (IL-6) and anti-tumor necrosis factor-alpha (TNF-α) were got from Wanleibio (Shengyang, China). IL-1β, IL-6 and TNF-α ELISA kits were purchased from ELK Biotechnology CO., Ltd. (Wuhan, China). Oil red O staining kit was purchased from Solabio (Beijing, China). LY294002 and ISRIB were bought from Med Chem Express (Shanghai, China). Alanine aminotransferase (ALT), TG and aspartate aminotransferase (AST) kits were bought from Mindray (Shenzhen, China).