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3 protocols using 6 hydroxy 2 5 7 8 tetramethychroman 2 carboxylicacid

1

Chromatographic Analysis of Antioxidant Compounds

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Chromatographic-grade methanol
and ethanol were used for HPLC analyses (VWR, Austria). Ammonium acetate,
copper(II) chloride, glacial acetic acid, sodium acetate trihydrate,
ferric chloride hexahydrate, quercetin, 6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic
acid (Trolox), and reagents 2,2-diphenyl-1-picrylhydrazyl (DPPH),
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS),
neocuproine (2,9-dimethyl-1,10-phenanthroline), and 2,4,6-tripyridyl-s-triazine (TPTZ) were purchased from Sigma-Aldrich.
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2

Defatting and Antioxidant Evaluation of Rice Bran

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Kum Doi Saket rice bran was purchased from a local grinding mill company (Organic Germinated Brown Rice Community Enterprise, Chiang Mai, Thailand) and defatted using hexane7 ,8 (link). Chemicals used in the experiments, including 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid (Trolox), sulfur trioxide–trimethylamine (STMA), anhydrous dimethyl sulfoxide (DMSO), D-glucose and ascorbic acid (vitamin C), were purchased from Sigma-Aldrich Co. Ltd. (St. Louis, MO, USA). Other reagents in this experiment were analytical grade purchased from Union Science Co., Ltd. (Chiang Mai, Thailand).
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3

Antioxidant Compound Characterization

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DPPH, ABTS, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 3,3′,5,5′-tetramethyl-benzidine (TMB), 6-hydroxy-2,5,7,8-tetramethy-chroman-2-carboxylic acid (Trolox), acetic acid, aluminum nitrate, (+)-catechin, ferric chloride, Folin-Ciocalteau reagent, potassium persulfate, quercetin, silibinin, silymarin, sodium carbonate, sodium hydroxide, sodium hypochlorite, sodium molybdate, and sodium nitrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Apigenin, diosmetin, and verbascoside were purchased from Extrasynthese (Genay, France). Isosilibin, silicristin, and silydianin were purchased from SunHank Technology Co., Ltd. (Tainan, Taiwan). Hydrogen chloride, HPLC-grade methanol, and o-phosphoric acid were purchased from Merck (Darmstadt, Germany).
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