MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazoliumbromide) was obtained from Fluka Chemical Corp (Ronkonkoma, NY, USA) and was dissolved in 0.01 M phosphate-buffered saline (PBS). Antibodies against RIPK1(A7414), RIPK3(A5431), MLKL(A5579), HIF-1α(A16873) were purchased from Abclonal Technology (Wuhan, China). Antibodies against, HSP90α(BS6461), were products of Bioworld Technology (USA). Antibodies against AFP (4448) and LAMP2 (49067) were products of Cell Signaling Technology (Beverly, MA, USA). Antibodies against Lamin A (sc-293162), β-Tubulin (sc-166729) and β-Actin (sc-8432) were products of Santa Cruz Biotechnology (USA). Normal mouse and rabbit IgG-HRB secondary antibodies were purchased from Santa Cruz Biotechnology.
Normal mouse and rabbit igg hrb secondary antibodies
Normal mouse and rabbit IgG-HRB secondary antibodies are used to detect and quantify target proteins in various immunoassays. They bind to the primary antibody, which is specific to the target protein, and the attached horseradish peroxidase (HRP) enzyme allows for colorimetric or chemiluminescent detection.
Lab products found in correlation
2 protocols using normal mouse and rabbit igg hrb secondary antibodies
Dissecting Cell Death Pathways
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazoliumbromide) was obtained from Fluka Chemical Corp (Ronkonkoma, NY, USA) and was dissolved in 0.01 M phosphate-buffered saline (PBS). Antibodies against RIPK1(A7414), RIPK3(A5431), MLKL(A5579), HIF-1α(A16873) were purchased from Abclonal Technology (Wuhan, China). Antibodies against, HSP90α(BS6461), were products of Bioworld Technology (USA). Antibodies against AFP (4448) and LAMP2 (49067) were products of Cell Signaling Technology (Beverly, MA, USA). Antibodies against Lamin A (sc-293162), β-Tubulin (sc-166729) and β-Actin (sc-8432) were products of Santa Cruz Biotechnology (USA). Normal mouse and rabbit IgG-HRB secondary antibodies were purchased from Santa Cruz Biotechnology.
Investigating Cell Death Mechanisms
Antibodies against RIPK1, RIPK3, MLKL, were purchased from Abclonal Technology (Wuhan, China). Antibodies against HIF-1α, HSP90α, p-MLKL, and GAPDH were products of Bioworld Technology (USA).
Antibodies against AFP and LAMP2 were products of Cell Signaling Technology (Beverly, MA, USA). Antibodies against Lamin A, β-Tubulin and β-Actin were products of Santa Cruz Biotechnology (USA).
Normal mouse and rabbit IgG-HRB secondary antibodies were purchased from Santa Cruz Biotechnology.
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