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Normal mouse and rabbit igg hrb secondary antibodies

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Normal mouse and rabbit IgG-HRB secondary antibodies are used to detect and quantify target proteins in various immunoassays. They bind to the primary antibody, which is specific to the target protein, and the attached horseradish peroxidase (HRP) enzyme allows for colorimetric or chemiluminescent detection.

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2 protocols using normal mouse and rabbit igg hrb secondary antibodies

1

Dissecting Cell Death Pathways

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Sorafenib (Bay 43-9006, Sigma-Aldrich), 17-AAG (Tanespimycin, MCE, HSP90α inhibitor), Z-VAD-FMK (Selleck, Caspase Inhibitor), Necrostatin-1 (Selleck, RIPK1 inhibitor), Necrosulfonamide (Selleck, MLKL inhibitor).
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazoliumbromide) was obtained from Fluka Chemical Corp (Ronkonkoma, NY, USA) and was dissolved in 0.01 M phosphate-buffered saline (PBS). Antibodies against RIPK1(A7414), RIPK3(A5431), MLKL(A5579), HIF-1α(A16873) were purchased from Abclonal Technology (Wuhan, China). Antibodies against, HSP90α(BS6461), were products of Bioworld Technology (USA). Antibodies against AFP (4448) and LAMP2 (49067) were products of Cell Signaling Technology (Beverly, MA, USA). Antibodies against Lamin A (sc-293162), β-Tubulin (sc-166729) and β-Actin (sc-8432) were products of Santa Cruz Biotechnology (USA). Normal mouse and rabbit IgG-HRB secondary antibodies were purchased from Santa Cruz Biotechnology.
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2

Investigating Cell Death Mechanisms

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Sorafenib Bay 43-9006, Sigma-Aldrich), 17-AAG (Tanespimycin, MCE, HSP90 inhibitor), Z-VAD-FMK (Selleck, Caspase Inhibitor), Necrostatin-1 (Selleck, RIPK1 inhibitor) Necrosulfonamide (Selleck, MLKL inhibitor). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazoliumbromide) was obtained from Fluka Chemical Corp (Ronkonkoma, NY, USA) and was dissolved in 0.01 M phosphate-buffered saline (PBS).
Antibodies against RIPK1, RIPK3, MLKL, were purchased from Abclonal Technology (Wuhan, China). Antibodies against HIF-1α, HSP90α, p-MLKL, and GAPDH were products of Bioworld Technology (USA).
Antibodies against AFP and LAMP2 were products of Cell Signaling Technology (Beverly, MA, USA). Antibodies against Lamin A, β-Tubulin and β-Actin were products of Santa Cruz Biotechnology (USA).
Normal mouse and rabbit IgG-HRB secondary antibodies were purchased from Santa Cruz Biotechnology.
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