Siglec f bv421 clone e50 2440

Manufactured by BD

Siglec F (BV421; clone E50-2440) is a flow cytometry reagent used for the detection and identification of siglec-F-expressing cells. Siglec-F is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family and is primarily expressed on eosinophils. This reagent can be used to label and study eosinophil populations in various biological samples.

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Lab products found in correlation

Facsdiva v8, by BD (1 mentions) Lsrfortessa x 20, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Cd11c alexa fluor 700 clone hl3, by BD (1 mentions) F4 80 apc cy7 clone bm8, by BioLegend (1 mentions) Cytexpert software v 2, by Beckman Coulter (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

Spelling variants of this product

Siglec-F (101 citations) E50-2440 (19 citations) Siglec-F BV421 (9 citations)

2 protocols using siglec f bv421 clone e50 2440

Immunophenotyping of Colonic Leukocytes

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Large intestine samples were removed, open longitudinally, and gently washed with ice-cold PBS. Tissue was cut into small pieces and washed twice in predigestion solution (1X HBSS, 5 mM EDTA, 1 mM DTT) for 20 min at 37 °C with gentle shaking. Tissue was then enzymatically digested in collagenase D (0.05 g/100 ml PBS). Collected fractions were pooled within sample and lysed to remove red blood cells. Staining of single-cell suspensions included the following antibodies from BD: Ly6C (FITC; clone AL-21), CD11b (PerCP-Cy5.5; clone M1/70), Siglec F (BV421; clone E50-2440), CD11c (Alexa Fluor 700; clone HL3), CD45.2 (BUV395; clone 30-F11), EpCAM (PE; clone G8.8), Ly6G (PE-Cy7; clone 1A8), and MHCII (BV711; clone M5/11.415.2). CD169 (eFluor 660; clone SER-4) was purchased from eBioscience, and F4/80 (APC/Cy7; clone BM8) was purchased from BioLegend. DAPI (Life Technologies) was used as a viability control. Samples were collected on BD LSRFortessa X-20 with the use of BD FACSDiva v.8.0 software for data acquisition, analyzed using FlowJo v.10.2 software, and enumerated by back calculating based off of percentages relative to total cell counts.

McElrath C., Espinosa V., Lin J.D., Peng J., Sridhar R., Dutta O., Tseng H.C., Smirnov S.V., Risman H., Sandoval M.J., Davra V., Chang Y.J., Pollack B.P., Birge R.B., Galan M., Rivera A., Durbin J.E, & Kotenko S.V. (2021). Critical role of interferons in gastrointestinal injury repair. Nature Communications, 12, 2624.

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Flow Cytometry Analysis of BAL Cells

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Cells collected from the BALF were stained as previously published (18 (link)) for LIVE/DEAD, CD11c-PECy7 (clone N418), Ly6G-APCCy7 (clone 1A8), Ly6C-PerCPCy5.5 [clone HK1.4; (BioLegend, San Diego, CA)], Siglec F-BV421 [clone E50–2440; (BD Biosciences, San Jose, CA)]. For intracellular YM1 (STEMCELL Technologies, Vancouver, BC) or AR (Cell Signaling Technology, Danvers, MA) detection, cells were stained with a secondary PE-anti-rabbit antibody (BioLegend). For the DC stain, CD209a-PE (clone MMD3), CD80-PE (clone 16–10A1), and CD86-AlexaFluor700 [clone GL-1; (BioLegend)], were used. Stained cells were analyzed with a CytoFLEX flow cytometer (Beckman-Coulter, Brea, CA), courtesy of the Anesthesiology and Critical Care Medicine Flow Cytometry Core. CytEXPERT software v.2.0 (Beckman-Coulter) was used for analysis.

Becerra-Díaz M., Strickland A.B., Keselman A, & Heller N.M. (2018). Androgen and androgen receptor as enhancers of M2 macrophage polarization in allergic lung inflammation. Journal of immunology (Baltimore, Md. : 1950), 201(10), 2923-2933.

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