The mesenteric tissue was collected from rats at the end of the in vivo experiment.26 (link) Deposits of β2GPI were analyzed using the biotinylated monoclonal antibody MBB2 and FITC-labeled streptavidin (Sigma-Aldrich).27 (link) IgG and C3 were detected using FITC-labeled goat anti-human IgG (Sigma-Aldrich) and goat anti-rat C3 (Cappel/MP Biomedicals) followed by FITC-labeled rabbit anti-goat IgG (Dako), respectively. The slides were examined using a DM2000 fluorescence microscope equipped with a DFC 490 photo camera and Application Suite software (Leica).
Fitc labeled rabbit anti goat igg
Manufactured by Agilent Technologies
Sourced in Denmark
FITC-labeled rabbit anti-goat IgG is a secondary antibody that binds to goat immunoglobulin G (IgG) and is labeled with the fluorescent dye fluorescein isothiocyanate (FITC). This product can be used in various immunoassay and imaging techniques that require the detection of goat IgG.
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Lab products found in correlation
2 protocols using fitc labeled rabbit anti goat igg
1
Mesenteric Tissue Analysis for β2GPI Deposits
2
Immunohistochemical Analysis of Kidney and Heart
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Ergidina binding to kidney or heart samples was evaluated using frozen sections (7 µm) that had been incubated with the antibody (10 µg/ml) for 60 min at room temperature followed by FITC-labeled goat anti-human IgG (Aczon, Monte SanPietro, Bologna, Italy).
Tissue deposition of C3 was assessed by incubating frozen kidney 7 µm sections with 1:200 goat IgG anti-rat C3 (Cappel, ICN Biomedicals, Milan, Italy) for 60 min at room temperature followed by FITC-labeled rabbit anti-goat IgG at a 1:200 dilution (DAKO, Glostrup, Denmark) for additional 60 min at room temperature. A similar approach was used to evaluate the presence of C9, using rabbit IgG anti-rat C9 (a kind gift from Prof. P. Morgan, Cardiff, UK) at a 1:1,000 dilution, followed by FITC-labeled swine anti-rabbit IgG (DAKO) at a 1:40 dilution.
The fluorescence intensity was evaluated in 10 different randomly selected areas (0.07 mm2 each) of renal tissue. ImageJ image analysis software (National Institutes of Health) was used.
Tissue deposition of C3 was assessed by incubating frozen kidney 7 µm sections with 1:200 goat IgG anti-rat C3 (Cappel, ICN Biomedicals, Milan, Italy) for 60 min at room temperature followed by FITC-labeled rabbit anti-goat IgG at a 1:200 dilution (DAKO, Glostrup, Denmark) for additional 60 min at room temperature. A similar approach was used to evaluate the presence of C9, using rabbit IgG anti-rat C9 (a kind gift from Prof. P. Morgan, Cardiff, UK) at a 1:1,000 dilution, followed by FITC-labeled swine anti-rabbit IgG (DAKO) at a 1:40 dilution.
The fluorescence intensity was evaluated in 10 different randomly selected areas (0.07 mm2 each) of renal tissue. ImageJ image analysis software (National Institutes of Health) was used.
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