Heparin sulfate

Manufactured by R&D Systems

Heparin sulfate is a highly sulfated glycosaminoglycan that is found on the surface of many cell types. It plays a key role in various biological processes, including cell signaling, growth factor regulation, and extracellular matrix organization.

Automatically generated - may contain errors

Lab products found in correlation

Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Fibroblast growth factor 2 (fgf2), by R&D Systems (1 mentions) Heparin sulfate, by Merck Group (1 mentions) Rhfgf4, by Thermo Fisher Scientific (1 mentions) Pd0325901, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Heparin (18 citations)

2 protocols using heparin sulfate

Glioblastoma Tumor Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Surgical samples from patients with newly diagnosed or recurrent glioblastoma were obtained from the Tumor Tissue Bank within the Arnie Charbonneau Cancer Institute, transported to the BTIC Core Facility (Calgary, Alberta) and established as described previously^{44 (link), 45 (link)}. This study has Institutional review board approval under the “Brain Tumor and Related Tissue Bank protocol-V2” and approved by Foothills Hospital and the Conjoint Health Research Ethics Board and all methods were performed in accordance with the relevant guidelines and regulations. All established cell lines used were validated for identity by short tandem repeat analysis performed by Calgary Laboratory Services. All BTIC lines were grown in serum free-media supplemented with EGF (20 ng/ml; Peprotech, FGF2 (20 ng/ml; R&D Systems Inc, and heparin sulfate (2 mg/ml: R&D Systems). For the collection of conditioned media (CM) individual BTIC lines were plated at one million cells/ml, after 48 hrs media was collected and centrifuged to remove debris.

Deng J., Koutras C., Donnelier J., Alshehri M., Fotouhi M., Girard M., Casha S., McPherson P.S., Robbins S.M, & Braun J.E. (2017). Neurons Export Extracellular Vesicles Enriched in Cysteine String Protein and Misfolded Protein Cargo. Scientific Reports, 7, 956.

+ Open protocol

+ Expand

Modulating FGF/ERK Pathway in ESCs & Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

For activation of the FGF/ERK pathway in ESCs, 10–100 ng/ml of recombinant human FGF2 (rhFGF2, CellGS) or FGF4 (rhFGF4, Peprotech) was added to the specified culture medium with 1 μg/ml of Heparin Sulfate (Sigma). To inhibit FGF/ERK activity in ESC cultures, cell culture medium was supplemented with the MEK inhibitor (MEKi) PD0325901 (Stemgent) at 15–1000 nM, as specified in the figures and figure legends.
For activation of the FGF/ERK pathway in pre-implantation embryos, 1 μg/ml of recombinant human FGF4 (rhFGF4, R&D Systems) and 1 μg/ml of Heparin Sulfate were diluted in culture medium (KSOM-AA) and embryos cultured as described above. Control embryos were cultured in KSOM-AA supplemented with 1 μg/ml of Heparin Sulfate. Similarly, for inhibition of FGF/ERK activity, either a MEK inhibitor (MEKi) PD0325901, or FGF receptor inhibitor (FGFRi) AZD4547 (Santa Cruz) were diluted to 1 μM in culture medium, and embryos grown as described for the periods indicated in the corresponding figures.

Morgani S.M., Saiz N., Garg V., Raina D., Simon C.S., Kang M., Arias A.M., Nichols J.N., Schröter C, & Hadjantonakis A.K. (2018). A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Developmental biology, 441(1), 104-126.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!