Brightfield images of lungs from in vivo and in vitro experiments were captured either on a Leica MZ 125 stereoscopic dissecting microscope using a Spot Insight 2.0 Mp Color Mosaic camera and Spot 4.5.9 imaging software, or were obtained from live imaging experiments using a Leica DM6000B inverted microscope, DFC 305FX camera, and Leica Application Suite Advanced Fluorescence imaging software.
Dfc 305fx camera
Manufactured by Leica
The DFC 305FX is a camera designed for laboratory applications. It features a high-resolution sensor and provides accurate color reproduction for scientific imaging tasks.
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2 protocols using dfc 305fx camera
1
Brightfield Imaging of Lung Samples
2
Quantifying Lung Branching Dynamics
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Brightfield images of lungs from in vivo and in vitro experiments were captured either on a Leica MZ 125 stereoscopic dissecting microscope using a Spot Insight 2.0 Mp Color Mosaic camera and Spot 4.5.9 imaging software, or were obtained from live imaging experiments using a Leica DM6000B inverted microscope, DFC 305FX camera, and Leica Application Suite Advanced Fluorescence imaging software. Fluorescence intensities were quantified using FIJI software (version 2.0.0-rc-68/1.52 g).
Branching was quantified by counting and averaging the distal tips of the left lobe of control and experimental samples at 0 and 24 h. These groups were analyzed using a two-factorial quasi-Poisson model, with the dispersion parameter estimated as 0.317. Differences were considered statistically significant at p < 0.05.
Branching was quantified by counting and averaging the distal tips of the left lobe of control and experimental samples at 0 and 24 h. These groups were analyzed using a two-factorial quasi-Poisson model, with the dispersion parameter estimated as 0.317. Differences were considered statistically significant at p < 0.05.
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