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F 3000 fluorescence spectrophotometer

Manufactured by Hitachi
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Sourced in Japan

The Hitachi F-3000 fluorescence spectrophotometer is a laboratory instrument designed to measure the fluorescence properties of samples. It is capable of detecting and quantifying the intensity of fluorescent light emitted by molecules in a sample when excited by a light source.

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Lab products found in correlation

Fura 2 am fluorescence dye, by Thermo Fisher Scientific (2 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions) Unity 300 mhz spectrometer, by Agilent Technologies (1 mentions) U 4100, by Hitachi (1 mentions)

4 protocols using f 3000 fluorescence spectrophotometer

1

Chemokine-induced Calcium Signaling Assay

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The procedure has been described previously (Nakayama et al., 2010 (link)). Briefly, cells were loaded with 3 μM fura 2-AM fluorescence dye (Thermo Fisher Scientific). After washing, the cells were placed on a F3000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan) and stimulated with each recombinant human chemokine. Emission fluorescence at 510 nm was measured upon excitation at 340 and 380 nm, and the fluorescence intensity ratio (R340/380) was obtained.
Matsuo K., Koizumi K., Fujita M., Morikawa T., Jo M., Shibahara N., Saiki I., Yoshie O, & Nakayama T. (2016). Efficient Use of a Crude Drug/Herb Library Reveals Ephedra Herb As a Specific Antagonist for TH2-Specific Chemokine Receptors CCR3, CCR4, and CCR8. Frontiers in Cell and Developmental Biology, 4, 54.
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2

Quantifying Total Cellular ROS Levels

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Total ROS level was determined by the oxidative conversion of nonfluorescent 2′, 7′-dichlorofluorescein diacetate (H2DCFDA) to highly fluorescent 2′, 7′-dichlorofluorescein (DCF) as described previously [25] (link). Liver extracts of amount 100 µl were incubated at 37°C for 60 min with 100 µl of 2 mM H2DCFDA (Invitrogen) in PBS. Fluorescence was recorded at 485 nm (excitation) and 527 nm (emission) with HITACHI F-3000 fluorescence spectrophotometer. The level of ROS in each sample was determined by observing fluorescence (absorbance)/mg protein.
Das L, & Vinayak M. (2014). Long Term Effect of Curcumin in Regulation of Glycolytic Pathway and Angiogenesis via Modulation of Stress Activated Genes in Prevention of Cancer. PLoS ONE, 9(6), e99583.
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3

Intracellular Calcium Imaging with Fura-2

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The procedure has been described previously (18 (link)). Briefly, cells were loaded with 3 μM fura 2-AM fluorescence dye (Thermo Fisher Scientific). After washing, cells were placed on F3000 Fluorescence Spectrophotometer (Hitachi, Tokyo, Japan) and stimulated with recombinant chemokines. Emission fluorescence at 510 nm was measured upon excitation at 340 and 380 nm, and the fluorescence intensity ratio (R340/380) was obtained.
Matsuo K., Kitahata K., Kawabata F., Kamei M., Hara Y., Takamura S., Oiso N., Kawada A., Yoshie O, & Nakayama T. (2018). A Highly Active Form of XCL1/Lymphotactin Functions as an Effective Adjuvant to Recruit Cross-Presenting Dendritic Cells for Induction of Effector and Memory CD8+ T Cells. Frontiers in Immunology, 9, 2775.
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4

Synthesis and Characterization of Luminescent Platinum Complexes

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All reagents were commercially available and used as supplied without further purification. 4-bromo-N,N-bis(p-(pyrid-4-yl)phenyl)aniline58 (link) and diplatinum acceptor Pt-1 and Pt-2 were prepared according to the reported procedures.29 (link) 1H NMR and 13C NMR spectra were recorded in the designated solvents on a Varian Inova 500 or 400 MHz spectrometer. 31P{1H} NMR spectra were recorded on a Varian Unity 300 MHz spectrometer, using an external unlocked sample of 85% H3PO4 (δ = 0) as reference. Mass spectra were recorded on a Micromass Quattro II triple-quadrupole mass spectrometer using electrospray ionization with a MassLynx operating system. Absorption and fluorescence spectra were recorded on a Hitachi U-4100 and Hitachi F-7000 Spectrophotometer, equipped with 1 cm quartz cuvette from Starna Cells, Inc. The temperature-dependent fluorescent experiments were conducted on a Hitachi F-3000 fluorescence spectrophotometer. The luminescence lifetime was obtained on Quantaurus-Tau Fluorescence lifetime spectrometer C11367 from Hamamatsu Photonics.
Tang J.H., Sun Y., Gong Z.L., Li Z.Y., Zhou Z., Wang H., Li X., Saha M.L., Zhong Y.W, & Stang P.J. (2018). Temperature-Responsive Fluorescent Organoplatinum(II) Metallacycles. Journal of the American Chemical Society, 140(24), 7723-7729.
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