The human choriocarcinoma BeWo cell line was purchased from the JCRB Cell Bank (Osaka, Japan), whereas the human HTR8/SVneo invasive-type trophoblast cell line was kindly provided by Dr Charles Graham (Queen’s University Kingston, ON, Canada). BeWo cells were cultured in Ham’s F-12/DMEM (1:1; Fujifilm Wako Pure Chemical Corp., Osaka, Japan). HTR8/SVneo cells were cultured in Roswell Park Memorial Institute 1640 medium (Fujifilm Wako Pure Chemical Corp.). The media were supplemented with 10% fetal bovine serum (FBS; Nichirei Biosciences, Tokyo, Japan) and 1% penicillin-streptomycin-neomycin antibiotic mixture (Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained at 37 °C under 5% CO2 in a humidified incubator.
Bewo cell line
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
The BeWo cell line is a human choriocarcinoma cell line that is derived from placental tissue. It is a widely used in vitro model for studying various aspects of placental biology and function.
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2 protocols using bewo cell line
1
Cultivation of Human Choriocarcinoma and Trophoblast Cell Lines
2
Syncytialization of BeWo Choriocarcinoma Cells
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The human choriocarcinoma BeWo cell line, purchased from the JCRB Cell Bank (Osaka, Japan), were grown in 1:1 Ham’s F12/Dulbecco’s modified Eagle’s medium (Fujifilm Wako Pure Chemical Corp., Osaka, Japan) supplemented with 10% FBS and 1% PSA (Fujifilm Wako Pure Chemical Corp.) at 37 °C in humidified air containing 5% CO236 (link). the cells were treated with dibutyryl cAMP (500 μM, Tokyo Chemical Industry, Tokyo, Japan) or forskolin (2.5 µM, Cayman chemical, Ann Arbor, MI USA) an adenylate cyclase activator, for 48 h to induce syncytialization. These cells were also treated for 48 h with Que (5 µM, Tokyo Chemical Industry).
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