All microscopy experiments were done in M9 minimal medium (Miller, 1972 ) supplemented with 1 mM of MgSO4 (Sigma, M2773) and glycerol (0.2%) as carbon source. If not otherwise indicated we used NH4Cl (19 mM) as nitrogen source. Alternatively, for slower growth, we used Proline (Acros, AC157620250) (10 mM). The composition of M9 minimal medium is: Disodium Hydrogenophosphate (Na2HPO4, S7907, Sigma) (42 mM); Potassium Dihydrogen phosphate (KH2PO4, P0662, Sigma) (22 mM); Sodium Chloride (NaCl 31434, Sigma) (8.6 mM).
M2773
Manufactured by Merck Group
The M2773 is a laboratory equipment product offered by the Merck Group. It functions as a device for performing various laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
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Lab products found in correlation
2 protocols using m2773
1
Microscopy in M9 Minimal Medium
2
Preparation of Defined Microbial Growth Medium
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To milli-Q water (1 L) were added KH2PO4 (13.60 g, Sigma-Aldrich, Cat.#P5655), (NH4)2SO4 (2.00 g, Sigma-Aldrich Cat.# A4418) and glucose (2.00 g, Sigma-Aldrich Cat.#G7021), and the pH of the solution was adjusted to 7.00 ± 0.09 with KOH. The medium was then autoclaved at 121 °C with a 30 min sterilization cycle. After the solution cooled down to room temperature, syringe filter-sterilized (0.22 μm nitrocellulose syringe filters) solutions of MgSO4 (1 mL, 0.2 g mL−1 of MgSO4·7H2O, Sigma-Aldrich Cat. #M2773) and FeSO4 (500 μL, 1.0 mg mL−1 of FeSO4·7H2O, Sigma-Aldrich Cat.#F8633) were added to the medium broth. The medium was stored in the dark at room temperature until use.
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